Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
2.
Evaluation of a rapid molecular method for detection of
Listeria monocytogenes directly from broth culture -
R. A. Mathakiya, A. Roy, K. N. Nandasana, P. G. Koringa and C.G.
Joshi
Vet World. 2009; 2(5): 177-178
Abstract
The present
study was carried out to know the lowest detection limit of
Listeria monocytogenes by PCR. The quantification of
organisms was done by CFU counting from ten fold serial dilution
and PCR amplification of inlA gene fragment was performed
from each dilution. The PCR could detect as low as 20 organisms
indicating the lowest PCR detection limit. Thus, lowest number
of L. monocytogenes detectable by PCR is a low-cost and
rapid procedure that can be appropriated for the detection in
real time of low L. monocytogenes levels in naturally
contaminated food and is suitable to implement in the food
industry.
Keywords:Quantification,
CFU counting, Serial dilution, PCR, Low-cost, Food industry.
