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Comparison of excretory-secretory
antigen and positive faecal supernatant antigen in the
detection of Echinococcus granulosus infection in dogs by CIEP
Prathiush, K. J. Ananda, and Placid E. D’Souza
Coproantigen detection of Echinococcosis in dogs by
counter immunoelectrophoresis was standardized.
Adult Echinococcus granulosus worms were obtained
from intestine of a necropsied positive dog.
Excretory-secretory antigen was prepared by
culturing adult worms in Medium 199 (pH 7.4). Faeces
of positive dog were collected and fecal supernatant
was prepared and used for coproantigen detection.
CIEP was carried out using tris-borate buffer (pH
8.0) at a constant current of 8mA/slide for 60
minutes. CIEP detected infection with both the
Keywords: Echinococcus granulosus,
Excretory-secretory antigen, Coproantigen, Parasitic