Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
3.
Comparison of excretory-secretory
antigen and positive faecal supernatant antigen in the
detection of Echinococcus granulosus infection in dogs by CIEP
-
P. R.
Prathiush, K. J. Ananda, and Placid E. D’Souza
Veterinary
World 2(11):421-422
Abstract
Coproantigen detection of Echinococcosis in dogs by
counter immunoelectrophoresis was standardized.
Adult Echinococcus granulosus worms were obtained
from intestine of a necropsied positive dog.
Excretory-secretory antigen was prepared by
culturing adult worms in Medium 199 (pH 7.4). Faeces
of positive dog were collected and fecal supernatant
was prepared and used for coproantigen detection.
CIEP was carried out using tris-borate buffer (pH
8.0) at a constant current of 8mA/slide for 60
minutes. CIEP detected infection with both the
antigens.
Keywords: Echinococcus granulosus,
Excretory-secretory antigen, Coproantigen, Parasitic
Zoonosis.
