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Research
4.
In vitro
anthelminthic activity of
alcoholic extract of Allivum Sativum against rumen
amphistome -
G. R. Ghangale, Tushar Mahale and
N. D. Jadhav
Veterinary
World 2(10):385-386
Abstract
The present investigation was undertaken to evaluate effect of
alcoholic extract of allivum sativum for anthelminthic activity
Invitro against rumen amphistome. Mature and healthy worms
randomly collected from rumen papillae of freshly slaughtered
buffaloes from deonar abattor in normal physiological solution
(tyrode solution), having temp (37 0C).The study was conducted
at four different dilutions of extracts viz. 5,15,50,75 mg/ml
prepared in tyrode solution. Albendazole 5 mg/ml and tyrode
solution serves as positive control and negative control
respectively. Each of the concentration selected was taken in
total volume of 20 ml tyrode solution in a separate clean
petridish. Ten vigorously motile worms irrespective of their sex
were placed in each petridish and observation were made after
every 15 min. for cessation of motility by pinch method
mortality was assessed by slightly warming the petridish and
observing the movements. The time taken for cessation of
motility in alcoholic extract was 3 hour, 1hour, 10min., and
instant at 5,15,50,75 mg/ml concentration respectively.
Mortality was observed at 4hr, 1.5 hr, 15 min. and instantly
respectively in test groups. Time taken for 100% mortality was 6
hour by standard drug.So it was concluded that alcoholic extract
of Allivum Sativum is having good activity against worms’
motility Invitro.
Keywords:
Anthelmintic, Allivum Sativum, Parasite, Rumen, Alcohol.
