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Research
1.
Comparative efficacy of conventional diagnostic methods and
evaluation of polymerase chain reaction for the diagnosis of
bovine brucellosis -
Raheela Akhtar, Zafar Iqbal Chaudhry, A. R Shakoori, Mansoor ud
Din Ahmad and Asim Aslam
Vet World. 2010; 3(2): 53-56
Abstract
The comparative
efficacy of Rose Bengal Plate Test (RBPT) and Milk Ring test (MRT)
was calculated in terms of sensitivity and specificity for the
diagnosis of bovine brucellosis in cows (Group A) and buffaloes
(Group B) from Lahore and Okara districts of Punjab, Pakistan.
Using bacterial growth as a gold standard RBPT showed high
sensitivity values of 100% in both groups. While its specificity
was 96.29% (Group A) and 90.62% (Group B). On the other hands
MRT showed low sensitivity (80.0% in Group A; 86.6% in Group B)
while its specificity was 100% in all the animals of both
groups. The calculated positive predictive and negative
predictive values of both groups were in correspondence with
their specificity and sensitivity values respectively. High
sensitivity and low specificity of RBPT as compare to high
specificity and low sensitivity of MRT in all groups suggested
the poor efficacy of both tests used individually as compare to
bacterial growth. In the continuation of this study polymerase
chain reaction (PCR) was evaluated for its diagnostic efficacy
of quick Brucella abortus isolation from same samples. PCR
conducted on serum samples gave more positive results than on
milk samples. Therefore, the combination of both conventional
tests along with serum PCR can be recommended.
Key words: Diagnostic tests, PCR, Bovine brucellosis,
Laboratory Tests
