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Research
3.
Detection of Escherichia coli O157:H7 prevalence in foods
of animal origin by cultural methods and PCR technique -
Ch. Bindu Kiranmayi, N. Krishnaiah
Vet World. 2010; 3(1): 13-16
doi: 10.5455/vetworld.2010.13-16
Abstract
A total of
250 samples (50 each of beef, mutton and chicken and 50 samples
each of beef swabs and mutton swabs) collected from various
sources were subjected to PCR and cultural methods for the
presence of Escherichia coli O157:H7. Primers for hlyA,
stx1 & 2 genes were used for the detection of Escherichia coli
O157:H7 and shiga toxins
respectively. Out of 250 samples, 27 showed presence of
Escherichia coli O157:H7 (5 beef, 6 beef swabs, 2 mutton, 12
mutton swabs and 2 chicken out of 50 samples each) by PCR where
as only 11 samples (one beef, 2 beef swabs, 1 mutton, 6 mutton
swabs and one chicken sample) were positive by cultural method.
Of the 27 Escherichia coli O157:H7 positive samples by PCR, 12
showed stx1, 7 showed stx2 and 5 showed both stx1 and stx2. The
sensitivity of PCR method for Escherichia coli O157:H7 was
1.7cfu. Enrichment with mTSB broth
containing novobiocin gave
good results compared to mEC broth
with novobiocin by
both PCR and cultural methods.
Keywords:
Escherichia coli O157:H7, PCR, Laboratory Technique, Prevalence,
Cultural method.
