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Research
1.
Rapid, sensitive and cost effective method for
isolation of viral DNA from faecal samples of dogs -
Savi., Minakshi and Prasad, G.
Vet World. 2010; 3(3): 105-106
Abstract
A simple method for viral DNA extraction using
chelex resin was developed. The method used was
eco-friendly and cost effective compared to other
methods such as phenol chloroform method which use
health hazardous organic reagents. Further, a
polymerase chain reaction (PCR) based detection of
canine parvovirus (CPV) using primers from conserved
region of VP2 gene was developed. To increase the
sensitivity and specificity of reaction, nested PCR
was designed. PCR reaction was optimized to amplify
747bp product of VP2 gene. The assay can be
completed in few hours and doesn’t need hazardous
chemicals. Thus, the sample preparation using
chelating resin along with nested PCR seems to be a
sensitive, specific and practical method for the
detection of CPV in diarrhoeal faecal samples.
Keywords: Chelating resin, CPV, Nested PCR,
Virus, Sensitivity, Faecal sample.
