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Case Report
14.
Autologous stem cell therapy to treat chronic ulcer
in heifer- A case study - Jayakrushna Das, Indramani Nath, Rabindra Kumar Das,
Padmanav Routray, Sidhartha Sankar Behera
Vet World. 2012; 5(12): 771-774
doi:
10.5455/vetworld.2012.7
71-774
Abstract
Aim:
The study was conducted to reveal the efficacy of
Bone marrow derived mesenchymal stem cells (BM-MSCs)
based therapy in healing of chronic non-healing and
ulcerative wound in bovine species.
Materials and
Methods: One 2 years old Jersey heifer affected
with chronic ulcerative wound involving full
thickness skin and under lying muscle at dorsal side
of lumbar region since four months at the time of
presentation. Bone marrow was collected from tibia,
cultured and grown and after achievement of optimum
confluence it was applied at the site. Different
parameters of clinical, physiological,
haematological, biochemical, histochemical,
histological, tensile strength and photographic
evaluations were done during the study period.
Results: The estimated values of above mentioned
parameters on zero day and after healing (18 days)
showed significant difference (P<0.05) in relation
to collagen content, tensile strength and physical
characteristics of wound like extent of wound, size
of wound, type of exudates and photography. But
clinical, haematological and biochemical data showed
no significant difference.
Conclusion: The
BM-MSCs were the main pioneers to bring the chronic
ulcerative wound towards healing. The procedure is
simple, safe and effective in bringing out healing
without showing any adverse effect on host.
Keywords: BM-MSCs, bovine, chronic ulcerative
wound
