Veterinary World


ISSN (Online): 2231-0916


 Editorial board

 Instructions for authors

 Reviewer guideline

 Open access policy





Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Original Research

3. Sex determination of cattle meat by polymerase chain reaction amplification of the amelogenin (AMELX/AMELY) gene - P. Gokulakrishnan, R. R. Kumar, B. D. Sharma, S. K. Mendiratta, D. Sharma , O. P. Malav
Vet World. 2012; 5(9): 526-529

Aim: The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing of cattle meat based on the amelogenin (AMELX/AMELY) gene using PCR technique which is superior to earlier work in terms of band patterns.

Materials and Methods: Based on the amelogenin gene located on the conservation region of X and Y chromosomes, a pair of primers was designed and the system of PCR was established to amplify a 241-bp fragment from the X chromosome in female cattle, and a 241-bp fragment from X chromosome and 178-bp fragment from the Y chromosome in male cattle, respectively. The accuracy and specificity of the primers was assessed using DNA template extracted from cattle meat samples of known sex. The protocol was subjected to a blind test showed 100% concordance, proving its accuracy and reliability.

Results: PCR products of cattle meat samples after electrophoresis showed two bands (241, 178-bp) for tissue from male while female tissue resulted in only one (241-bp) band.

Conclusions: Our findings show that the PCR assay based on the amelogenin gene is reliable for sex determination in cattle meat.

Keywords: Amelogenin gene, Cattle meat, PCR, Sex determination