Veterinary World


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Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Original Research

3. Isolation and partial characterization of lytic phage against Salmonella Abortusequi - Harshit Verma, Divya Pramod, Mohd. Abbas, Awadhesh Prajapati, Durairajan Ramchandra and Mayank Rawat
Vet World. 2013; 6(2): 72-75

Aim: To use partially characterized phages for generation of phage lysates having potential to be developed as vaccines against S. Abortusequi.

Materials and Methods: Phage/s isolation from several sewage samples was attempted against Salmonella Abortusequi strains procured from the National Salmonella Reference Centre, IVRI using enrichment and agar overlay technique. A concentrated stock of the phage was prepared after its purification. The lytic range, morphology and DNA size were determined.

Results: Two lytic phages could be isolated and purified. The phage with wider lytic range was found to possess a hexagonal head with a very short tail. The DNA size was found to be 21kb.

Conclusion: The investigation reports first isolation of a lytic phage against Salmonella Abortusequi. The phage can be used in future for development of phage lysate based immune therapeutic agent against equine abortions.

Keywords: bacteriophage, lytic phage, Salmonella Abortusequi