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Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online : 23-10-2013)

17. Occurrence of multidrug resistance shiga-toxin producing Escherichia coli from milk and milk products - Javeed Ahmad Sheikh, Mohd. Rashid, Majueeb U Rehman and M. A. Bhat

Veterinary World, 6(11): 915-918

 

doi: 10.14202/vetworld.2013.915-918

 

 

 

 

Abstract

Aim: The aim of this study was to assess the hygienic quality of milk and milk products in respect of shiga toxin producing Escherichia coli (STEC).
Materials and Methods: 205 samples of raw milk and milk products were processed for isolation of E. coli. The isolates were screened by mPCR for detection of virulence gene. 52 E. coli isolates were tested against 15 commonly used antibiotics in the field.
Results: Of the 205 samples of milk and milk products, 52 (25.36%) were positive for E. coli. Out of which 4% samples were positive for STEC from raw milk and 3.64% from milk products. Also, 25 of 52 (48%) of E. coli isolates were multidrug resistance whereas 62.5% of STEC from milk and milk products were multidrug resistance.
Conclusions: The milk and milk products production is of poor hygienic quality in and around Jammu region which needs improvement.
Key words: milk, mPCR, multidrug resistance, STEC

References

1. Solomakos, N., Govaris, A., Angelidis A. S. (2009) Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece. Food Microbiology, 26: 865–871.
http://dx.doi.org/10.1016/j.fm.2009.06.002
PMid:19835773
 
2. Armstrong, G.L., Hollingsworth, J. and Morris, J.G.J. (1996) Emerging food borne pathogen: E. coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world. Epidemiology Review, 18: 29-51.
http://dx.doi.org/10.1093/oxfordjournals.epirev.a017914
PMid:8877329
 
3. Karch, H., Schmidt, H., Brunder, W. (1998) Plasmid- encoded determinants in E.coli O157:H7.in:kaper, J.B., O, Brien, A.D., (Eds), E.Coli o157:H7 and other shiga toxin producing E.coli strains. ASM press, Washington, D.C., pp.183-194.
 
4. Griffin, P.M. and Tauxe. R.V. (1991)The epidemiology of infections caused by Escherichia coli O157:H7, other enterohaemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiology Review, 13:60–98.
PMid:1765120
 
5. Griffin, P.M. (1995)Escherichia coli O157: H7 and other hemorrhagic Escherichia coli. In infection of the gastrointestinal tract, edited by M.J. Blaser, P.D. Smith, J.I. Ravdin, H.B. Greenberg and R.I. Guerrant, pp. 739-761,New York, N.Y.Raven Press, Ltd.
 
6. Health protection agency (2010) Identification of E. coli O157. National standard method BSOP ID 22 issue 3. http://www.hpa-standardmetods.org.uk/pdf_sops.asp, Accessed on 1st Sept 2013.
 
7. Riley, L.W., Remis R.S., Helgerson S.D., McGee, H.B., Wells, J.G., Gavis B.R., Hebert R.J., Olcott E.S., Johnson, L.M., Hargrett, N.T., Blake, P.A. and Cohen, M.L. (1983) Haemorrhagic colitis associated with rare Escherichia coli Serotypes. New England Journal of Medicine, 308: 681-685.
http://dx.doi.org/10.1056/NEJM198303243081203
PMid:6338386
 
8. Hitchins, A.D., Hartman, P.A., Todd, E.C.D. (1992) Coliform Escherichia coli and its toxins. In: Vanderzant, c., Splittstoesser, D. (Eds), compendium of methods for the Microbiological Examination of foods. pp. 327-329. Compiled by the APHA Technical Committee on Microbio- logical Methods for Foods.
 
9. Quinn, P.J., Carter, M.E., Markey, B.K. and Carter, G.R. (1994) Clinical Veterinary Microbiology, pp. 21-26 and 209- 236. Published by Wolfe Publishing, an imprint of Mosby- Year Book Europe Limited Printed in Spain by Grafos, S.A. Arte SobrePapel ISBN 0723417113.
 
10. Paton, A.W., and Paton,J.C. (1998) Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. Journal of Clinical Microbiology, 36:598-602.
PMid:9466788 PMCid:PMC104589
 
11. Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turck, M. (1966) Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology, 45(4): 493-496.
PMid:5325707
 
12. Daood, N. (2007) Detection and antimicrobial susceptibility ofE.coliO157: H7 in raw bovine milk, some of dairy products and water samples. Damascus University Journal for BasicSciences, 23:1-15.
 
13. Rashid, M., Kotwal, S.K., Malik, M.A and Singh, M. (2013) Prevalence, genetic profile of virulence determinants and multidrug resistance of E. coli isolates from foods of animal origin, Vet. World 6(3):139-142.
http://dx.doi.org/10.5455/vetworld.2013.139-142
 
14. Soomro, A.H., Arain, M.A., Khaskheli, M. and Bhutto, B. (2002)Isolation of Escherichia Coli from Raw Milk and Milk Products in Relationto Public Health Sold under Market Conditions at Tandojam. Pakistan Journal of Nutrition, 1(3): 151-152.
http://dx.doi.org/10.3923/pjn.2002.151.152
 
15. Ali, A. A. and Abdelgadir, W.S. (2011) Incidence of Escherichia coli in Raw Cow's Milk in Khartoum State. British Journal of Dairy Sciences,2(1): 23-26.
 
16. Adesiyun, A.A. (1994) Bacteriological quality and associated public health risk of pre-processed bovine milk in Trinidad. International Journal of Food Microbiology, 21:253-261.
http://dx.doi.org/10.1016/0168-1605(94)90032-9
 
17. Njage, P.M.K., Jans, C., Wangoh, J., Lacroix, C. and Meile,L. (2012) Detection, isolation and molecular characterisation of Shigatoxigenic O157 and non-O157 Escherichia coli in raw and fermented camel milk. African Journal of Microbiology, 6(31):6031-6038.
 
18. Steele, M.L., Mcnab, W.B., Poppe, C., Griffiths, M.W., Chen, S., Degrandis, S.A., Eruhner, L.C., Larkin, C.A., Lynch, J.A. and Odumeru, J.A. (1997) Survey of Ontario bulk tank raw milk for food-borne pathogens. Journal of Food Protection, 60: 1341-1346.
 
19. Chye, F.Y., Abdullah, A., Ayob, M.K. (2004) Bacteriological quality and safety of raw milk in Malaysia. Food Microbiology, 21:535-541.
http://dx.doi.org/10.1016/j.fm.2003.11.007
 
20. Vicente, H.I.G., Amaral, L.A., Cerqueira, A.M.F. (2005) ShigatoxigenicEscherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms. Brazil journal Microbiology, 36: 217-222.
 
21. Hussein, H.S., Sakuma, T. (2005) Prevalence of Shiga toxin producing Escherichia coli in dairy cattle and their products. Journal Dairy Science, 88: 450-465.
http://dx.doi.org/10.3168/jds.S0022-0302(05)72706-5
 
22. Rangel P. and Marin, J.M. (2009)Analysis of Escherichia coli isolated from bovine mastitic milk. Pesquisa Veterinaria Brasileira, 29(5): 363-368.
http://dx.doi.org/10.1590/S0100-736X2009000500001
 
23. Rahimi, E., Chaleshtori, S.S. and Parsaei, P. 2011.Prevalence and antimicrobial resistance of Escherichia coli O157 isolated from traditional cheese, ice cream and yoghurt in Iran. African Journal of Microbiology Research, 5(22): 3706-3710.
 
24. Momtaz, H., Farzan, R., Rahimi, E., Dehkordi, F.S. and Souod, N. (2012) Molecular Characterization of Shiga Toxin-Producing Escherichia coli Isolated from Ruminant and Donkey Raw Milk Samples and Traditional Dairy Products in Iran. The Scientific World Journal, 10:1-13.
http://dx.doi.org/10.1100/2012/231342
PMid:22919299 PMCid:PMC3419413