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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 19-08-2013)
15. PCR detection and serotyping of enterotoxigenic and shigatoxigenic
Escherichia coli isolates obtained from chicken meat in Mumbai, India
- R. J. Zende, D. M. Chavhan, P. R. Suryawanshi, A. K. Rai and V. M. Vaidya
Veterinary World, 6(10): 770-773
doi:
10.14202/vetworld.2013.770-773
Abstract
Aim: Present study was
undertaken to find out the frequency of few virulent genes and
prevalence of related strains of Escherichia coli isolated from
chicken meat obtained from chicken retail shops by Polymerase
Chain Reaction (PCR).
Materials and Methods: 66 samples of freshly slaughtered
chicken meat were collected from 22 identified retail shops
located at Mumbai city, randomly. Processed meat samples were
cultured in EMB agar and presumptive colonies were confirmed by
various biochemical tests. PCR method was accustomed for
identification of the genes coding for heat-stable enterotoxin a (STa),
heat labile enterotoxin (LT), shiga-like toxins 1 and 2 (SLT1 and
SLT2). E. coli isolates were sent to National Salmonella and
Escherichia Centre, CRI, Kasauli, HP, India for serotyping.
Results: 11 (16.67%) E. coli strains were isolated from 66
chicken meat samples. 3 (27.27%) out of 11 harbored the gene for
SLT2, and 2 (18.18%) for STa. None of the strain contains SLT1 and
LT genes. Serotypes detected were rough, O2, O20, O22, O102 each
for one isolate and 6 isolates were untypable (UT).
Conclusion: The results concluded that chicken meat samples
analysed harbored genes for shiga like toxins and enterotoxins and
different serotypes of E. coli. These findings indicating that
regular monitoring of chicken meat is essential for this pathogen
to prevent potential public health problems.
Keywords: chicken meat, enterotoxin, Escherichia coli,
polymerase chain reaction, virulence gene
References
|
1. Udaykar, A., Sharda, R., Chhabra, D. and Sharma, V. (2009)
Study of virulence factors of Escherichia coli strains
isolated from diarrhoeic calves. Indian J. Comp. Microbiol.
Immunol. Infect. Dis., 30: 26-28. |
|
|
|
2. Chansiripornchai, N. (2009) Comparative efficacy of en-
rofloxacin and oxytetracycline by different administra-tion
methods in broilers after experimental infection with avian
pathogenic Escherichia coli. Thai J. Vet. Med., 39: 231–236. |
|
|
3. Ferens, W.A. and Hovde, C.J. (2011) Escherichia coli
O157:H7: animal reservoir and sources of human infection.
Food-borne Pathog. Dis., 8: 465–485.
http://dx.doi.org/10.1089/fpd.2010.0673
PMid:21117940 PMCid:PMC3123879 |
|
|
4. Nataro, J.P. and Kaper, J.B. (1998) Diarrheagenic
Escherichia coli. Clin. Microbiol. Rev., 11: 142-201.
PMid:9457432 PMCid:PMC121379 |
|
|
|
5. Nweze, E.I. (2009) Virulence properties of diarrheagenic E.
coli and etiology of diarrhea in infants, young children and
other age groups in southeast, Nigeria. American-Eurasian J.
Sci. Res., 4: 173-179. |
|
|
|
6. Gyles, C.L. and Fairbrother, J.M. (2004) Escherichia coli.
pages: 160-193. In: Gyles, CL;Prescott, JF; Songer, JG and
Theon, CO (Eds.), Pathogenesis of bacterial infections in
animals. (3rd Edn.), Ames, Iowa, Blackwell Publishing. PP:
145-214. |
|
|
7. Coombes, K.B., Wickham, E.M., Mascarenhas, M., Gruenheid,
S., Finlay, B.B. and Karmali, A.M. (2008) Molecular analysis
as an aid to assess the public health risk of non-O157 shiga
toxin-producing Escherichia coli strains. Appl. Environ
Microbiol., 74: 2153-2160.
http://dx.doi.org/10.1128/AEM.02566-07
PMid:18245257 PMCid:PMC2292595 |
|
|
8. Pradel, N., Bertin, Y., Martin, C. and Livrelli, V. (2008)
Molecular analysis of shiga toxin-producing Escherichia coli
strains isolated from hemolytic uremic syndrome patients and
dairy samples in France. Appl. Environ. Microbiol., 74:
2118-2128.
http://dx.doi.org/10.1128/AEM.02688-07
PMid:18245246 PMCid:PMC2292610 |
|
|
9. Chapman, P., Chapman, A., Cerdan Malo, A.T., Ellin, M.,
Ashton, R. and Harkin, M.A. (2001) Escherichia coli O157 in
cattle and sheep at slaughter, on beef and lamb carcasses and
in raw beef in raw beef and lamb products in South Yorkshire,
UK Int. J. Food Microbiol., 64:139-150.
http://dx.doi.org/10.1016/S0168-1605(00)00453-0 |
|
|
10. Omisakin, F., Macrae, M., Ogden, I.D. and Strachan, N.J.
(2003) Concentration and prevalence of Escherichia coli O157
in cattle feces at slaughter. Appl. Environ. Microbiol., 69:
2444-2447.
http://dx.doi.org/10.1128/AEM.69.5.2444-2447.2003
PMid:12732509 PMCid:PMC154535 |
|
|
|
11. Wilson, K. (1987) Preparation of genomic DNA from
bacteria. Current protocols in molecular biology, Unit 2.4.1,
New York, Wiley. |
|
|
|
12. Pollard, D.R., Johnson, W.M., Lior, H., Tyler, S.D. and
Rozee, K.R. (1990) Rapid and specific detection of verotoxin
genes in Escherichia coli by the polymerase chain reaction. J.
Clin. Microbiol. 20: 540-545. |
|
|
|
13. Nguyen, T.L., Truong, H.T. and Nguyen, V.G. (2009)
Determining virulence factors of Escherichia strains isolated
from diarrhea piglets by using PCR method. J. Sci. Dev., 7:
187:191. |
|
|
|
14. Bonyadian, M., Moshtaghi, H., Nematalahi, A., Rahimi, E.,
Akhavan Taheri, M. and Karami, S. (2011) Isolation of
enterotoxigenic and enteroaggregative straines of Escherichia
coli from chicken carcasses by PCR. Iranian J. Vet. Res., 12:
252-255. |
|
|
15. Kiranmayi, C.B. and Krishnaiah, N. (2010) Detection of
Escherichia coli O157:H7 prevalence in foods of animal origin
by cultural methods and PCR technique. Veterinary World, 3:
13-16.
http://dx.doi.org/10.5455/vetworld.2010.382-389 |
|
|
|
16. Dutta, T.K., Roychoudhury, P., Sadhukhan, T.K., Ghosh, A.
and Bandyopadhaya, A.G. (2010) Detection and charact-
erization of shigatoxigenic E. coli (STEC) and entero-
pathogenic Escherichia coli (EPEC) from diarrhoeic piglets in
an organized farm in Kolkata. Indian J. Anim. Sci., 80:
493-496. |
|
|
17. Wani, S.A., Nabi, A., Fayaz, I., Ahmad, I., Nishikawa, Y.,
Qureshi, K., Khan M.A. and Chowdhary, J. (2006) Investi-
gation of diarrhoeic faecal samples for enterotoxigenic shiga
toxin-producing and typical or atypical enteropathogenic
Escherichia coli in Kashmir, India. FEMS Microbiol. Lett.,
261: 238-244.
http://dx.doi.org/10.1111/j.1574-6968.2006.00354.x
PMid:16907727 |
|
|
|
18. Danashree, B. and Mallya, P.S. (2008) Detection of shiga-
toxigenic Escherichia coli (STEC) diarroeagenic stool and meat
samples in Mangalore, India. Indian J. Med. Res., 128:
271-277. |
|
|
|
19. Vivegnis, J., Lioui, M., Leclercq, A., Lambert, B. and
Decallonne, J. (1999) Detection of Shiga-like toxin producing
Escherichia coli from raw milk cheeses produced in Wallonia.
Biotechnol. Agron. Soc. Environ., 3: 159-164. |
|
|
20. Coia, J., Johnston, Y., Steers, N. and Hanson, M. (2001) A
survey of the prevalence of Escherichia coli O157:H7 in raw
meats, raw cow's milk and raw-milk cheeses in south-east
Scotland. Int. J. Food Microbiol. 66: 63-69.
http://dx.doi.org/10.1016/S0168-1605(00)00490-6 |
|
|
21. Tutenel, A.V., Pierard, D., Van Hoof, J. Cornelis, M. and
De Zutter, L. (2003) Isolation and molecular characterization
of Escherichia coli O157 isolated from cattle, pigs and
chickens at slaughter. Int. J. Food Microbiol., 84: 63-69.
http://dx.doi.org/10.1016/S0168-1605(02)00395-1 |
|
|
|
22. Akkaya, L. Atabay, H.I., Kenar, B. and Alisarli, M. (2006)
Prevalence of verotoxigenic E. coli O157:H7 on chicken
carcasses sold in Turkey. Bul. Vet. Ins. Pulawy., 50: 513-516. |
|
|
23. Ghanbarpour, R., Salehi, M. and Oswald, E. (2010)
Virulance genotyping of E. coli isolates from avian cellulitis
in relation to phylogeny. Com. Clin. Pathol., 19: 147-153.
http://dx.doi.org/10.1007/s00580-009-0837-4 |
|
|
24. Cohen, N., Ennaji, H., Hassar, M. and Karib, H. (2006) The
bacterial quality of red meat and offal in Casablanca
(Morocco). Mol. Nut. Food. Res., 50: 557-562.
http://dx.doi.org/10.1002/mnfr.200500180
PMid:16676376 |
|
|
