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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online : 07-07-2013)
14. Multi-drug resistance among Shiga toxin producing Escherichia coli isolated
from bovines and their handlers in Jammu region, India -
Majueeb U Rehman, Mohd Rashid, Javeed Ahmad Sheikh, Shakil Ahmad Wani and
Shaheen Farooq
Vet World. 2013; 6(9):
655-658
doi:
10.14202/vetworld.2013.655-658
Abstract
Aim: The objective of this
study was to determine the antibiotic resistance pattern of Shiga
toxin-producing Escherichia coli (STEC) in bovines and their
handlers.
Materials and Methods: Of the total of 126 E. coli isolates
screened by multiplex PCR for the presence of Shiga-toxin genes,
15 STEC isolates were obtained comprising of 9 isolates from
cattle, 3 from buffaloes, and 3 from bovine handlers, which were
tested for their antibiotic sensitivity/ resistance pattern to
various antibiotics.
Results: Twelve of the 15 STEC isolates (80%) showed
resistance to three or more antibiotics. Chloramphenicol was the
most effective with 86.6% sensitivity, followed by Norfloxacin
(80%), Ciprofloxacin (73.3%), and Co-trimoxazole (73.3%). Whereas
66.6% of the STEC isolates were resistant to Amikacin and
Ampicillin, the other 60% were resistant to Amoxycillin, Cefixime,
and Kanamycin.
Conclusion: Multiple drug resistance patterns among the
STEC in the present study, especially high resistance to the
frequently used antibiotics in both bovines and their handlers,
implies that antibiotic resistance is often acquired due to their
indiscriminate use; thereby, creating a need for rational and
judicious use of antibiotics in the field.
Keywords: antibiotic sensitivity pattern, bovines, bovine
handlers, multi-drug resistance, STEC,
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