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Research
(Published
online: 02-08-2014)
1. A comparative evaluation of avidin-biotin ELISA and micro SNT
for detection of antibodies to infectious bovine rhinotracheitis
in cattle population of Odisha, India
- Priyaranjan Das,
Nihar Nalini Mohanty, S. Ranganatha, Siddharth Ranabijuli, Laxmi
Narayan Sarangi and Hemant Kumar Panda
Veterinary World, 7(8): 548-552
doi:
10.14202/vetworld.2014.548-552
Priyaranjan Das: Orissa Biological Products Institute (SatelliteUnit),
Berhampur, Odisha, India; drpriya.vety@gmail.com
Nihar Nalini Mohanty: Clinical Bacteriology Laboratory, Indian
Veterinary Research Institute, Mukteswar, Uttarakhand, India;
nihar.mohanty13@gmail.com
S. Ranganatha: National Institute of Veterinary
Epidemiology and Disease Informatics, Hebbal, Bengaluru-560024,
Karnataka, India; srangavetco@gmail.com
Siddharth Ranabijuli: Krishi Vigyan Kendra, Sakhigopal,
Odisha, India; drsidhu.vet@gmail.com
Laxmi Narayan Sarangi: Regional Medical Research Centre, Bhubaneswar,
Odisha, India; laxmisarangi@gmail.com
Hemant Kumar Panda: Department of Veterinary Microbiology,
Veterinary College, Orissa University of Agriculture and
Technology, Bhubaneswar, Odisha, India; drpanda.hk@gmail.com
Received: 09-04-2014, Revised: 17-06-2014, Accepted: 24-06-2014,
Published online: 02-08-2014
Corresponding author: Nihar Nalini Mohanty, email:
nihar.mohanty13@gmail.com
Abstract
Aim: The present study was
undertaken to serologically detect Infectious Bovine
Rhinotracheitis (IBR) in the cattle population of Odisha, India
using micro-Serum neutralization test (micro SNT) and Avidin-Biotin
Enzyme linked immuno sorbent assay (AB ELISA) and finding out
their comparative efficacy to serve as a suitable diagnostic tool
in field condition.
Materials and Methods: The study was carried out using
serum samples (n=180) collected randomly from cattle populations
of nine districts of Odisha. Similarly vaginal swabs (n=26) from
cattle having history of repeat breeding, abortion,
vulvo-vaginitis and nasal swabs (n=8) from calves with respiratory
symptoms and nasal discharge were collected aseptically, to
ascertain the circulation of virus among the cattle population.
Results: Virus isolation by cell culture and subsequent
confirmation by polymerase chain reaction confirmed four isolates.
Screening of serum samples revealed 9.44% and 12.22% samples
positive for IBR antibodies in micro SNT and AB ELISA
respectively. The sensitivity and specificity of AB ELISA test was
found to be 88.23% and 95.70% respectively taking micro SNT as
gold standard and the kappa value between the two tests was 0.75.
Conclusion: Screening of serum samples revealed 9.44% and
12.22% samples positive for IBR antibodies in micro SNT and AB
ELISA respectively, thus highlighting the circulation of virus
among the livestock population of Odisha and that AB ELISA could
be more efficiently applied for the sero-diagnosis of IBR virus
infections at field conditions, with demand for more study on
faster, efficient and large scale screening of the infected
animals.
Keywords: avidin-biotin ELISA, bovine herpes virus -1,
cattle, infectious bovine rhinotracheitis, isolation, serum
neutralization test, Odisha.
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