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Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online: 02-08-2014)

1. A comparative evaluation of avidin-biotin ELISA and micro SNT for detection of antibodies to infectious bovine rhinotracheitis in cattle population of Odisha, India - Priyaranjan Das, Nihar Nalini Mohanty, S. Ranganatha, Siddharth Ranabijuli, Laxmi Narayan Sarangi and Hemant Kumar Panda
Veterinary World, 7(8): 548-552



   doi: 10.14202/vetworld.2014.548-552


Priyaranjan Das:
Orissa Biological Products Institute (SatelliteUnit), Berhampur, Odisha, India;

Nihar Nalini Mohanty: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute, Mukteswar, Uttarakhand, India;

S. Ranganatha: National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru-560024, Karnataka, India;

Siddharth Ranabijuli: Krishi Vigyan Kendra, Sakhigopal, Odisha, India;

Laxmi Narayan Sarangi: Regional Medical Research Centre, Bhubaneswar, Odisha, India;

Hemant Kumar Panda: Department of Veterinary Microbiology, Veterinary College, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India;

Received: 09-04-2014, Revised: 17-06-2014, Accepted: 24-06-2014, Published online: 02-08-2014


Corresponding author: Nihar Nalini Mohanty, email:

1. MacLachlan, N. J and Dubovi, E. J. (2011) Fenner's Veterinary Virology. 4th Edn. Academic Press, London. p180.
2. Biswas S., Bandyopadhyay S., Dimri U and Patra P.H. (2013) Bovine herpesvirus-1(BHV-1) a re-emerging concern in livestock: a revisit to its biology, epidemiology, diagnosis, and prophylaxis. Vet Qurt., 33(2):68-81.
3. Bandyopadhaya, S., Chakraborty, D., Sarkar, T., Pal, B., Sasmal, D., Biswas, T.K., Ghosh, M.K. and Sarkar, M. (2009) A serological survey of antibodies against bovine herpes virus-1 in yak (Poephagus grunniens) in Arunachal Pradesh in India. Rev. sci. tech. Off. int. Epiz., 28 (3):1045- 1050.
4. Ganguly, S., Mukhopadhya, S. K. and Paul, I. (2008) Studies on seroprevalence of infectious bovine rhinotracheitis in cattle population of West Bengal. Indian J. Comp.Microbiol. Immunol. Infect. Dis., 29(1-2): 12-16.
5. Kiran K. K., Ravi, P. and Prabhudas, K. (2005) Infectious bovine rhinotracheitis- National survey of IBR antibodies by AB-ELISA kit. Annual Report of Project Directorate on Animal Disease Monitoring and Surveillance. ICAR, Bangalore. p7-10.
6. Reed, L. J. and Muench, H. (1938) A simple method of estimating fifty per cent endpoints. Amer. J. Hyg. 27: 493.
7. Tongaonkar, S. S., Singh, B. K. and Rama, K. (1986) Comparative prevalence of the infectious bovine rhinotracheitis, Bluetongue, Chlamydia and Brucella antibodies in dairy animals. Indian J. Comp. Microbiol. Immunol. Infect. Dis.,7: 139-143.
8. Suresh, K. B., Sudharshana, K. J and Rajasekhar, M. (1999). Seroprevalence of infectious bovine rhinotracheitis in India. Indian Vet. J., 76: 5-9.
9. Gungor, A. B and Ozkul, A. (2007). Dynamics of natural bovine herpesvirus-1 (BHV-1) infection in a dairy herd. Trop Anim Health Prod., 39: 1320.
10. Chandranaik, B. M., Chethana, S., Kumar, S and Renukaprasad, C. (2010) Isolation of BHV-1 from bovine semen and application of real-time PCR for diagnosis of IBR/IPV from clinical samples. Vet. Arhiv., 80: 467-475.
11. Nisavic, J., Milic, N., Aleksandra, K and Tanja, J. (2010) The application of polymerase chain reaction in detection of bovine herpes virus 1 in clinical samples. Acta Vet., 60 (1): 39-48.
12. Singh, B. K., Sreenivasan, M. A., Tongaonkar, S. S., Mukherjee, F and Shrivastava, P. K. (1989) Isolation of infectious bovine rhinotracheitis from two out breaks of respiratory tract infections in cattle. Indian J. Anim. Sci., 59 (6): 644-646.
13. Ross, C., Riquelme, M.E., Ohman, F. K. and Belak, S. (1999) Improved detection of five closely related ruminant alphaherpesviruses by specific amplification of viral genome sequences. J. Virol. Methods., 83: 5565.
14. Smits C.B., Van Maanen C., Glas R.D, De Gee A.L., Dijkstrab T., Van Oirschot J.T. and Rijisewijk F.A. (2000). Comparison of three polymerase chain reaction methods for routine detection of bovine herpesvirus 1 DNA in fresh bull semen. J. Virol. Methods, 85, 6573.
15. Anita, D., Anita, A. and Savuta, G.(2010). Detection of Bovine Herpesvirus Type 1 by PCR Assay. Bulletin UASVM, Veterinary Medicine., 67(2):23-27.
16. Kumar, M., Manohar, M. and Nandi, S.(2011). A rapid PCR for detection of BHV-1 genomic DNA in semen samples. Indian Vet. J., 88(6):14-16.
17. Bauer, K., Gerbermann, J. H., Schmittodiet, E. and Winteroll, G. (1980) Serological investigation into the distribution of IBR/IPV in cattle in Bavaria. Tierarztl Umschau, 35(9): 599- 600.
18. Wiyong, A. (1993) Study on the prevalence of antibody to infectious rhinotracheitis virus in sentinel Bali cattle and their calves in Lampung. Penyakit-Hewan, 25: 7-10.
19. Ceylan, A., Tan, M.T., Dagalp,S. B., Yildirim, Y. and Serin, I. (2009) Seroprevalence of IBR/IPV in dairy cows with reproductive problems. Indian Vet. J., 86 (1): 7-9.
20. Nandi, S., Kumar, M., Yadav, V and Chander, V. (2011) Serological evidences of bovine herpesvirus-1 infection in bovines of organized farms in India. Transbound Emerg Dis., 58: 105109.
21. Pandey, A. B., Mehrotra, M. L., Verma, R. P and Pati, U. S. (2000) Investigation of an outbreak of infectious pustular balanoposthitis in breeding bulls. Indian J. Vet. Res., 9: 27-37.
22. Renukaradhya, G. J., Rajasekhar, M. and Raghavan, R. (1996) Prevalence of infectious bovine rhinotracheitis in Southern India. Rev. Sci. Tech. Off. Int. Epiz., 15: 1021-1028.
23. Singh, A., Sinha, B. K. and Jayachandran, C. (2010) Seroprevalence of IBR in normal and diseased bovines. Indian Vet. J., 87 (4): 393-394.
24. Trangadia, B. J., Rana, S. K., Nagmani, K. and Srinivasan, V. A. (2012) Serological Investigation of Bovine Brucellosis, Johne's disease and Infectious Bovine Rhinotracheitis in Two States of India. J. Adv. Vet. Anim. Res., 2: 38-41.
25. Sharma, M., Katoch, R. C. and Dhar, P. (2006) Seroprevalance of IBR among cattle in Himachal Pradesh. Indian Vet. J., 83: 1-3.
26. Shome, B. R., Rajeswari, S. and Shrivastava, N. (1997) Sero- prevalence of antibodies to BHV-1 in cattle of Andaman. Indian Vet. J., 74: 734-736.
27. Dhand, N. K., Singh, G., Sharma, D. R. and Sandhu, K. S. (2002) Seroprevalence of IBR in Punjab. Indian J. Anim. Sci., 72: 850-852.
28. Boelaert, F., Biront, F., Soumare, B., Dispas, M., Vanopdenbosch, E., Vermeersch, J. P., Raskin, A., Dufey, J., Berkvens, D. (2000) Prevalence of bovine herpesvirus-1 in the Belgian cattle population. Prev Vet Med., 45: 285-295.