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Research
(Published
online: 26-12-2014)
19. Prevalence of Clostridium perfringens
toxin genotypes in enterotoxemia suspected sheep flocks of
Andhra Pradesh -
N. Vinod Kumar, D. Sreenivasulu and Y. N.
Reddy
Veterinary World, 7(12): 1132-1136
doi:
10.14202/vetworld.2014.1132-1136
N.
Vinod Kumar:
Department of Veterinary Microbiology, College of Veterinary
Science, Tirupathi, Andhra Pradesh, India; nagaram_vinod@yahoo.com
D.
Sreenivasulu:
Department of Veterinary Microbiology, College of Veterinary
Science, Tirupathi, Andhra Pradesh, India; dsreenivasulu10@gmail.com
Y. N.
Reddy: Department of Veterinary Microbiology, College of
Veterinary Science, Tirupathi, Andhra Pradesh, India;
drynreddy@rediffmail.com
Received: 31-07-2014, Revised: 11-11-2014, Accepted: 19-11-2014,
Published online: 26-12-2014
Corresponding author:
N. Vinod Kumar, e-mail: nagaram_vinod@yahoo.com
Abstract
Aim:
To identify the Clostridium perfringens toxin genotypes
prevailing in enterotoxemia suspected sheep flocks in Andhra
Pradesh by using multiplex PCR.
Materials and Methods: Intestinal scrapings were collected
from lambs below three months of age from flocks with similar
management from different Districts of Andhra Pradesh, in India. A
total of 208 samples were collected with 140 from enterotoxemia
suspected flocks and 68 from healthy flocks. Following processing
and culture of the samples, colonies were identified by
morphological and biochemical tests. All the clostridial isolates
were analyzed by multiplex PCR.
Results: C. pefringens were isolated from 97 out of 140
enterotoxemia suspected flocks (69.29%) and 27 out of 68 healthy
flocks (39.71 %). Genotyping of the 97 iolates by multiplex PCR
from enterotoxemia suspected flocks indicated C. perfringens
type A, C and D 67.01% (65 out of 97); 11.34% (11 out of 97)
and 21.65% (21 out of 97) respectively. Isolates from healthy
flocks indicated the presence of type A and D 92.59% (25 out of
27) and 7.40% (2 out of 27) respectively. Number of isolates from
enterotoxaemia suspected flocks were significantly high (P<0.001)
than healthy flocks. Type A is found to be predominant in both
enterotoxemia suspected and healthy flocks (67.01% and 92.59%).
Conclusions: Prevalence of C. perfringens type C was
reported for the first time in India. Clostridium perfringens
type D and type C were found to be the major causative types
for enterotoxemia.
Keywords: Clostridium perfringes,
enterotoxaemia, lamb mortality, multiplex PCR, Toxin typing.
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