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Research
(Published
online: 11-12-2014)
6.
Serum amyloid A and haptoglobin levels in crossbred cows with
endometritis following different therapy -
S. S. Biswal, S. Das, S. Balasubramanian, D.
N. Mohanty, K. Sethy and M. Dasgupta
Veterinary World, 7(12): 1066-1070
doi:
10.14202/vetworld.2014.1066-1070
S. S.
Biswal:
Department of Animal Reproduction, Gynaecology and Obstetrics,
Madras Veterinary College, Tamil Nadu Veterinary
and Animal
Sciences University, Chennai, Tamil Nadu, India; Department of
Animal Reproduction, Gynaecology and Obstetrics, College of
Veterinary Science and Animal Husbandry, Orissa University of
Agriculture and Technology, Bhubaneswar, Odisha, India;
shuvranshu06@gmail.com
S. Das:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Orissa
University of Agriculture and Technology, Bhubaneswar, Odisha,
India;
dassrinibas@gmail.com
S.
Balasubramanian:
Department of Animal Reproduction, Gynaecology and Obstetrics,
Madras Veterinary College, Tamil Nadu Veterinary
and Animal
Sciences University, Chennai, Tamil Nadu, India;
bala2716@yahoo.com
D. N.
Mohanty:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Orissa
University of Agriculture and Technology, Bhubaneswar, Odisha,
India;
mohantydn@gmail.com
K.
Sethy:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Orissa
University of Agriculture and Technology, Bhubaneswar, Odisha,
India;
babuivri@gmail.com
M.
Dasgupta:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science and Animal Husbandry, Orissa
University of Agriculture and Technology, Bhubaneswar, Odisha,
India;
mohuadgupta@gmail.com
Received: 07-08-2014, Revised: 27-10-2014, Accepted: 04-11-2014,
Published online: 11-12-2014
Corresponding author:
S. S. Biswal, e-mail: shuvranshu06@gmail.com
Abstract
Aim:
To determine the serum variations of two major acute phase
proteins, serum amyloid A (SAA) and haptoglobin (Hp) levels in
crossbred endometritis cows following pre and post
immunomodulation therapy.
Materials and Methods: 21 endometritis cows were randomly
assigned to three groups (n=7) and treated with three different
immunomodulators while seven healthy cows served as control.
Uterine flushing collected from all animals was subjected to
bacteriological study and serum samples were analyzed for SAA and
Hp by sandwich ELISA method.
Results: Escherichia coli was most prevalent
Gram-negative bacteria (6.02 × 106 CFU/ml) while
Staphylococcus (0.86 × 106 CFU/ml) and
Streptococcus (0.52 × 106 CFU/ml)
were most predominant Gram-positive species isolated from uterine
flushing. The pre-treatment SAA values (μg/ml) varied
significantly (p<0.01) between the treatment groups whereas no
difference was observed in post-treatment groups. No significant
difference (p<0.01) was observed for Hp values between the
treatment groups, but the mean SAA (μg/ml) and Hp (μg/ml) levels
were significantly (p<0.01) higher in pre-treatment when compared
to post-treatment within the groups.
Conclusion: In the diagnosis and monitoring of bovine
endometritis, both SAA and Hp might serve as reliable biomarkers.
Keywords: crossbred cows, endometritis,
haptoglobin, serum amyloid A.
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