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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 28-02-2014)
12. m-RNA profiling of HSP-70 under different tropical
stress conditions in various broilers - S. A. Amrutkar, V. K. Saxena
and S. Tomar
Veterinary World, 7(2): 100-107
doi:
10.14202/vetworld.2014.100-107
Abstract
Aim: The present experiment
was conducted to study the effect of different tropical stress
conditions on haemato-biological traits in various broiler strains
during 3 to 4 weeks of age. Introgressing some important major
genes likes Naked neck (Na) and Frizzle (F) into broiler germplasm
may substantially improve the heat tolerance.
Materials and Methods: The experiment was designed to
evaluate three indigenously developed broilers viz. CARIBRO-Tropicana
(Naked neck and Frizzle gene bearing), CARIBRO-Mritunjai (Naked
neck gene bearing) and CARIBRO-Vishal (Normal plumaged) under
different THI (i.e. 72, 85 and 91) for 4 hours daily for 7 days.
Total 324 broiler chicks (i.e. 36 chicks in each group) of 3 weeks
of age were used in this study.
Results: The m-RNA expression of HSP-70 gene was observed
highest in frizzle plumaged birds and lowest in normal plumaged
birds. The CARIBRO-Vishal showed highest stress as compared to
other group. Higher the THI more severe was the effect on the
traits. During the 7 day of exposure trial birds of all the
genetic group exhibited the phenomenon of acclimatization as
reveled by the averages of various traits at different days into
the exposure. The mRNA expression analysis of HSP-70 in liver
revealed higher expression levels at later days during the
exposure trials which indicated the phenomenon of memory (stress
memory) and acquired thermotolerance.
Conclusion: Among the three genetic groups, CARIBRO-Tropicana
exhibited highest means for HSP-70 production as well as tolerated
the heat stress in a better way; therefore CARIBRO- Tropicana was
adjudged to be the best genetic group for production under
tropical climate.
Keywords: broiler, HSP-70, mRNA, tropical stress.
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