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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 15-02-2014)
6. Gold nanoparticle: synthesis and characterization
- Harihar Nath Verma, Praveen Singh and R. M. Chavan
Veterinary World, 7(2): 72-77
doi:
10.14202/vetworld.2014.72-77
Abstract
Aim: The aim of present study
was to synthesize and characterize gold nanoparticles applicable
in therapeutics and diagnostics tool for different diseases in
particular for peste des petits ruminants (PPR).
Materials and Methods: Gold nanoparticles were synthesized
by the reduction of Au+3 (HAuCl4) to Au0. For this purpose, the
reduction reaction between tetrachloroauric acid (HAuCl4) and
trisodium citrate (Na3C6H5O7.2H2O) was used in an aqueous
solution. The initial gold concentration, trisodium citrate
concentration and temperature were changed and their effects on
particle size and size distribution were observed. At the end of
the reduction reaction, gold nanoparticles with narrow size
distribution were obtained. Zeta sizer and transmission electron
microscopy (TEM) were used to characterize and determine the size
and size distribution of the synthesized gold nanoparticles.
Results: In the present work optimal gold salt
concentration, trisodium citrate concentration and temperature
were found to be 20 mM, 1.5% and 970C respectively. The
morphology, size, and shape of synthesized gold nanoparticles were
characterized by using spectrophotometry, transmission electron
microscopy (TEM) and zeta sizer. In our study colloidal gold
nanoparticle of 15-20 nm size were obtained.
Conclusions: The uniform gold nanoparticles were
synthesized by the citrate reduction method. The effects of
various experimental parameters (Gold salt concentration,
trisodium citrate concentration and mixing rate) on its size and
size distribution at optimum pH and temperature were investigated.
The gold nanoparticle synthesized by this method has been used for
the preparation of gold-labeled lateral flow strip for peste des
petits ruminants (PPR) diagnosis.
Key words: chemical reduction, gold nanoparticles, sodium
citrate, tetrachloroauric acid
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