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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 22-01-2014)
7. ELISA based monitoring and analysis of tetracycline
residues in cattle milk in various districts of Punjab - Abhishek
Gaurav, J. P. S. Gill, R. S. Aulakh and J. S. Bedi
Veterinary World, 7(1): 26-29
doi:
10.14202/vetworld.2014.26-29
Abstract
Background: Tetracycline
antibiotics are widely used for prevention and control of disease.
However, the presence of tetracycline antibiotics residues in food
is a serious public health concern because of the harmful effects
of these residues on consumer's health. Therefore, it is necessary
to detect tetracycline residues in food of animal origin keeping
in view the food safety issues and problem related to antibiotic
resistance.
Aim: To investigate and monitor the occurrence of
tetracycline residues in cattle milk in five districts of Punjab.
Materials and Methods: In this study, a total of 133 cattle
milk samples were collected from dairies of 5 districts of Punjab
state, India in May, 2013 and were tested and analyzed for their
contamination status with tetracycline residues.
Results: Out of these 133 milk samples analyzed, 18 samples
were found to contain tetracycline residues. The percentages of
tetracycline detected in the samples from 5 districts were in the
range of 0-46.15%. The concentration of tetracycline residues in
cattle milk samples were found to be in the range 16-134.5 ppb.
Conclusions: Three samples exceeded the maximum recommended
tetracycline antibiotic residue levels (MRLs) as prescribed by the
European Union and the Codex Alimentarius Commission .
Keywords: antibiotic residues, ELISA, milk, Punjab,
tetracycline.
References
|
1. Bren, L. (2002) The Battle of the Bugs: Fighting Antibiotic
Resistance. USDA Consumer Magazine 2002 Jul – Aug. http://www.fda.gov.fdac/features/2002/402_bugs.html.
Accessed on 03-10-2013. |
|
|
|
2. Khachatourians, G.G. (1998) Agricultural use of antibiotics
and the evolution and transfer of antibiotic-resistant
bacteria. JAMC.,159:1128-1136. |
|
|
|
3. Simonsen G. S., Haaheim H., Dahl K. H., Kruse H., Lovseth
A., Olsvik O. and Sundsfjord A. (1998) Transmission of Van
A-type vancomycin resistant enterococciand Van A resistance
elements between chicken and humans at avoparcin-exposedfarms.
Microb Drug Resist Winter.,4(4): 313 -318. |
|
|
|
4. European Commission. (2009) Regulation (EC) No. 470/2009 of
the European parliament and of the council of 6 May 2009:
laying down community procedures for the establishment of
residue limits of pharmacologically active substances in
foodstuffs of animal origin, repealing council regulation
(EEC) No.2377/90 and amending directive 2001/82/EC of the
European parliament and of the council and regulation (EC) No.
726/2004 of the European parliament and of the council.
Of?cial Journal of the European Union., L152 ; 11-22. |
|
|
|
5. European Commission. (2010) Commission regulation (EU) No.
37/2010 of 22 December 2009: on pharmacolo- gically active
substances and their classi?cation regarding maximum residue
limits in foodstuffs of animal origin. Of?cial Journal of the
European Union., L15 ; 1-72. |
|
|
6. Aguilera-Luiz, M. M., Vidal, J. L. M., Romero-González, R.,
and Frenich, A. G. (2008) Multi-residue determination of
veterinary drugs in milk by ultra-high-pressure liquid
chromatography-tandem mass spectrometry. Journal of
Chromatography A., 1205; 10-16.
http://dx.doi.org/10.1016/j.chroma.2008.07.066 |
|
|
7. Bohm, D. A., Stachel, C. S., and Gowik, P. (2009) Multi-
method for the determination of antibiotics of different
substance groups in milk and validation in accordance with
Commission Decision 2002/657/EC. Journal of Chromato- graphy
A., 1216: 8217-8223.
http://dx.doi.org/10.1016/j.chroma.2009.06.058 |
|
|
8. Gaugain-Juhel, M., Delépine, B., Gautier, S., Fourmond, M.
P., Gaidin, V. and HurtaudPessel, D. (2009) Validation of a
liquid chromatography-tandem mass spectrometry screening
method to monitor 58 antibiotics in milk: a qualitative
approach. Food Additives and Contaminants., 26: 1459-1471.
http://dx.doi.org/10.1080/02652030903150575 |
|
|
9. Junza, A., Amatya, R., Barrón, D., and Barbosa, J. (2011)
Comparative study of the LCMS/MS and UPLC-MS/MS for the
multi-residue analysis of quinolones, penicillins and
cephalosporins in cow milk, and validation according to the
regulation 2002/657/EC. Journal of Chromatography B., 879:
2601-2610.
http://dx.doi.org/10.1016/j.jchromb.2011.07.018 |
|
|
10. Kaufmann, A. (2009) Validation of multiresidue methods for
veterinary drug residues; related problems and possible
solutions. Analytica Chimica Acta., 637: 144-155.
http://dx.doi.org/10.1016/j.aca.2008.09.033 |
|
|
11. Ortelli, D., Cognard, E., Jan, P. and Edder, P. (2009)
Comprehensive fast multiresidue screening of 150 veterinary
drugs in milk by ultra-performance liquid chromatography
coupled to time of ?ight mass spectrometry. Journal of
Chromatography B., 877: 2363-2374.
http://dx.doi.org/10.1016/j.jchromb.2009.03.006 |
|
|
12. Stolker, A. A. M., Rutgers, P., Oosterink, E., Lasaroms,
J. J. P., Peters, R. J. B. and Van Rhijn, J. A. (2008)
Comprehensive screening and quanti?cation of veterinary drugs
in milk using UPLC-ToF-MS. Analytical and Bioanalytical
Chemistry., 391: 2309-2322.
http://dx.doi.org/10.1007/s00216-008-2168-8 |
|
|
13. Cinquina, A. L., Longo, F., Anastasi, G., Giannetti, L.
and Cozzani, R. (2003) Validation of a high-performance liquid
chromatography method for the determination of oxytetra-
cycline, tetracycline, chlortetracycline and doxycycline in
bovine milk and muscle. Journal of Chromatography A.,
987(1-2): 227–233.
http://dx.doi.org/10.1016/S0021-9673(02)01446-2 |
|
|
14. Fritz, J. W. and Zuo, Y. (2007) Simultaneous determination
of tetracycline, oxytetracycline, and 4-epitetracycline in
milk by high-performance liquid chromatography. Food
Chemistry.,105(3): 1297–1301.
http://dx.doi.org/10.1016/j.foodchem.2007.03.047 |
|
|
15. Bohm, D. A., Stachel, C. S., and Gowik, P. (2009) Multi-
method for the determination of antibiotics of different
substance groups in milk and validation in accordance with
Commission Decision 2002/657/EC. Journal of Chromato- graphy
A., 1216: 8217-8223.
http://dx.doi.org/10.1016/j.chroma.2009.06.058 |
|
|
16. Le Bizec, B., Pinel, G., and Antignac, J. P. (2009)
Options for veterinary drug analysis using mass spectrometry.
Journal of Chromatography A., 1216, 8016-8034.
http://dx.doi.org/10.1016/j.chroma.2009.07.007 |
|
|
17. Stolker, A. A. M., Zuidema, T., and Nielen, M. W. F.
(2007) Residue analysis of veterinary drugs and
growth-promoting agents. Trends in Analytical Chemistry., 26:
967-979.
http://dx.doi.org/10.1016/j.trac.2007.09.008 |
|
|
18. Turnipseed, S. B., Andersen, W. C., Karbiwnyk, C. M.,
Madson, M. R., and Miller, K. E. (2008) Multi-class, multi-
residue liquid chromatography/tandem mass spectrometry
screening and con?rmation methods for drug residues in milk.
Rapid Communications in Mass Spectrometry., 22: 1467-1480.
http://dx.doi.org/10.1002/rcm.3532 |
|
|
19. Franek, M., and Diblikove, I. (2006) Broad-speci?city
immunoassays for sulfonamide detection: immunochemical
strategy for generic antibodies and competitors. Analytical
Chemistry., 78: 1559-1567.
http://dx.doi.org/10.1021/ac0514422 |
|
|
20. Lamar, J., and Petz, M. (2007) Development of a receptor-
based microplate assay for the detection of beta-lactam
antibiotics in different food matrices. Analytica Chimica Acta.,
586: 296-303.
http://dx.doi.org/10.1016/j.aca.2006.09.032 |
|
|
21. Pastor-Navarro, N., Maquieira, A., and Puchades, R. (2009)
Review on immunoanalytical determination of tetracycline and
sulfonamide residues in edible products. Analytical and
Bioanalytical Chemistry., 395: 907-920.
http://dx.doi.org/10.1007/s00216-009-2901-y |
|
|
22. Toldrá, F., and Reig, M. (2006) Methods for rapid
detection of chemical and veterinary drug residues in animal
foods. Trends in Food Science and Technology., 17: 482-489.
http://dx.doi.org/10.1016/j.tifs.2006.02.002 |
|
|
23. Zhang, H., and Wang, S. (2009) Review on enzyme-linked
immunosorbent assays for sulfonamide residues in edible animal
products. Journal of Immunological Methods., 350: 1-13.
http://dx.doi.org/10.1016/j.jim.2009.07.006 |
|
|
|
24. Navratilova, P., Borkovcova, I., Drackova, M., Janštova,
B., and Vorlova, L. (2009) Occurrence of tetracycline,
chlortetracycline, and oxytetracycline residues in raw cow's
milk, Czech J. Food Sci., 27: 379–385. |
|
|
|
25. Elizabeta, D., Zehra, H., Biljana, S., Pavle, S. and Risto,
V. (2011) Screening of veterinary drug residues in milk from
individual farms in Macedonia. Mac. Vet. Rev. 34(1): 5 - 13. |
|
|
26. Alomirah, H., Al-Mazeedi, H., Al-Zenki, S., Al-Aati, T.,
Al- Otaibi, J., Al-Batel, M. and Sidhu, J. (2007) Pevalence of
antimicrobial residues in milk and dairy products in the state
of Kuwait. Journal of Food Quality., 30: 745-763.
http://dx.doi.org/10.1111/j.1745-4557.2007.00157.x |
|
|
|
27. Bilandžic, N., Solomun, B., Varenina, K.I. and Jurkovic,
Z. (2011) Concentrations of veterinary drug residues in milk
from individual farms in Croatia. Veterinary drug residues in
milk, Mljekarstvo., 61 (3),: 260-267. |
|
|
28. Zhang, Y. D., Zheng, N., Han, R. W., Zheng, B. Q., Yu, Z.
N., Li, S. L., Zheng, S. S. and Wang, J. Q. (2014) Occurrence
of Tetracyclines, Sulfonamides, Sulfamethazine and Quinolones
in Pasteurized milk and UHT milk in China's Market. Food
Control., 36(1):238-242.
http://dx.doi.org/10.1016/j.foodcont.2013.08.012 |
|
|
29. Unusan, N. (2009) Occurrence of chloramphenicol,
streptomycin and tetracycline residues in ultra-heat-
treatment milk marketed in Turkey. Int J Food Sci Nutr.,
60(5): 359-64.
http://dx.doi.org/10.1080/09637480701664555 |
|
|
