Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916

ISSN (Print): 0972-8988


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online: 23-07-2014)

13. Prevalence and risk factor's analysis of bovine brucellosis in peri-urban areas under intensive system of production in Gujarat, India - M. D. Patel, P. R. Patel, M. G. Prajapati, A. N. Kanani, K. K. Tyagi and A. B. Fulsoundar
Veterinary World, 7(7): 509-516



   doi: 10.14202/vetworld.2014.509-516


M. D. Patel;
Livestock Research Station, Navsari Agricultural University, Navsari-396 450, Gujarat, India;

P. R. Patel: Retired Professor and Head, Department of Veterinary Medicine, Veterinary College, Anand Agricultural University, Anand - 388001, Gujarat, India;

M. G. Prajapati:  Deputy Director, State Department of Animal Husbandry, Navsari District Panchayat, Navsari, Gujarat, India;

A. N. Kanani: Deputy Director, Animal Disease Investigation Office, State Department of Animal Husbandry, Polytechnic campus, Ambavadi, Ahmedabad, Gujarat, India;

K. K. Tyagi: Livestock Research Station, Navsari Agricultural University, Navsari-396 450, Gujarat, India;

A. B. Fulsoundar; Livestock Research Station, Navsari Agricultural University, Navsari-396 450, Gujarat, India;

Received: 31-03-2014, Revised: 14-06-2014, Accepted: 18-06-2014, Published online: 23-07-2014


Corresponding author: M. D. Patel, email:

1. Renukaradhya, G.J., Isloor, S. and Rajasekhar, M. (2002) Epidemiology, zoonotic aspects, vaccination and control/eradication of brucellosis in India. Vet. Microbiol. 90: 183-195.
2. FAO (2003) Guidelines for coordinated human and animal brucellosis surveillance. FAO Animal production and health paper-156. Rome. p45 (cited from Accessed on 28-08-2013.
3. Radostits, O.M., Gay, C.C., Blood, D.C. and Hinchcliff, K.W. (2000) Diseases caused by Brucella spp. Veterinary Medicine: a textbook of the diseases of cattle, sheep, pigs, goats, and horses, 9th Ed. W.B. Saunders, New York, p867- 882.
4. Batra, H.V., Chand P., Mukherjee, L.G.R. and Sadana, J.R. (1989) Dot-enzyme linked immunosorbent assay for detection of antibodies in bovine brucellosis. Res. Vet. Sci. 46: 143-146.
5. OIE (World Organisation for Animal Health). (2009) Bovine brucellosis, Section 2.4.3. In OIE Terrestrial Manual. OIE, Paris. p165 (Online available on Accessed on 28-08-2013.
6. Snecdor, G.W. and Cochran, W.G. (1994) Statistical Methods. Indian edition (Revised). Oxford and IBH Publishing Co., New Delhi. p439.
7. Lopez, J., Best, A. and Morales, C. (1998) Diagnosis of bovine brucellosis in milk by the ring test and ELISA in dairies in the province of Nuble, Chile. Arch. Med. Vet. 30(1):133-138.
8. Vanzini, V.R., Aguirre, N.P., Valentini, B. and Echaide, S.T. (2003) Determination of the quality control limits and adjustment of the cut off point for an indirect ELISA applied to the diagnosis of bovine brucellosis milk samples. Rev. Med. Vet. 84(5): 204-210.
9. Gumber, S., Aradhana, Dhand, N.K. and Sandhu, K.S. (2004) Village-level study of bovine brucellosis in Punjab (India) by bulk milk analysis. Indian J. Anim. Sci. 74: 843- 844.
10. Tun, T.N., Tharavichitkul, P., Kreausukon, K. and Tenhagen, B. (2007) Bovine brucellosis in dairy cattle in Yangon, Myanmar. In Proceedings: 15th Congress of the Federation of Asian Veterinary Associations FAVA-OIE Joint Symposium on emerging diseases, Bangkok, Thailand, 27- 30 October, 2008. p263-264.
11. Asfaw, Y., Molla, B., Zessin, K.H. and Tegegne, A.A. (1998) Cross-sectional study of bovine brucellosis and test performance in intra-and peri-urban production systems in and around Addis Ababa, Ethiopia. Bull. Anim. Health Prod. Africa. 46(4): 217-224.
12. Varasada, R.V. (2003) Seroprevalence of Brucellosis in cattle, buffaloes and human being in central Gujarat. M.V.Sc. thesis submitted to Gujarat Agricultural University, Gujarat, India. p171.
13. Singh, G., Sharma, D.R. and Dhand, N.K. (2004) Seroprevalence of bovine brucellosis in Punjab. Indian Vet. J. 81: 620-623.
14. Jaianandh, M., Ganesan, P.I., Jayakumar, R. and Senthilkumar, T.M.A. (2006) Bovine brucellosis in certain districts of Tamilnadu. Indian Vet. J. 83(9): 1025-1026.
15. Jadav, P.V., Bhanderi, B.B., Vadaviya, R.A. and Kavani, H.J. (2007) Study of prevalence of bovine brucellosis at village level by MRT method. Indian J. Environ. Toxicol. 17(2): 40-41.
16. Patel, T.J. (2007) Serological, cultural and molecular detection of Brucella infection in bovines including quantification in milk by real time PCR. M.V.Sc. thesis submitted to Anand Agricultural University, Anand, Gujarat, India. p105.
17. Aulakh, H.K., Patil, P.K., Sharma, S., Kumar, H., Mahajan, V. and Sandhu, K.S. (2008) A study on the epidemiology of bovine brucellosis in Punjab (India) using milk-ELISA. Acta Vet. Brno. 77(3): 393-399
18. Sharma, R.K., Arun Kumar, Thapliyal, D.C. and Singh, S. P. (2003) Sero-epidemiology of brucellosis in bovines. Indian J. Anim. Sci. 73(11): 1235-1237.
19. Shafee, M., Masood, R., Ahmad, S. A., Mansoor, A. and Abdul, R. (2011) Prevalence of bovine brucellosis in organized dairy farms using milk ELISA in Quetta City, Balochistan, Pakistan. Vet. Med. Intern. 2011, Article ID 358950, 3 pages, 2011. doi:10.4061/2011/358950.
20. Ali, S., Ali, Q., Abatih, E.N., Ullah, N., Ali, M., Khan, I. and Akhter, S. (2013) Sero-prevalence of Brucella abortus among dairy cattle and buffaloes in Pothohar Plateau, Pakistan. Pakistan J. Zool. 45(4): 1041-1046.
21. Priyadarshini, A., Sarangi, L. N., Palai, T. K., Panda, H. K., Mishra, R and Behera, P. C. (2013) Brucellosis in cattle and occupationally exposed human beings: a sero-survey in Odisha, India. J. Pure Appl. Microbiol. 7(4): 3255-3260.
22. Dhand, N.K, Gumber, S., Singh, B.B., Aradhana, Bali, M. S., Kumar, H., Sharma, D.R., Singh, J. and Sandhu, K.S. (2005) A study on the epidemiology of brucellosis in Punjab (India) using survey toolbox. Rev. Sci. Tech. 24(3): 879-85.
23. Khurana, S.K., Srivastava, S.K. and Prabhudas, K. (2012) Seroprevalence of bovine brucellosis in Haryana by avidin- biotin serum ELISA and its comparison with RBPT and SAT. Indian J. Anim. Sci. 82(5): 448-450.
24. Priyadarshini, A., Sarangi, L. N., Palai, T. K., Ranabijuli, S., Panda, H. K. and Mishra, R (2012) Diagnostic tests for sero- prevalence of brucellosis in cattle. Indian Vet. J. 89(5): 86- 87.
25. Al-Majali, A.M., Talafha, A.Q., Ababneh, M.M. and Ababneh, M.M. (2009) Seroprevalence and risk factors for bovine brucellosis in Jordan. Vet. Sci. 10(1): 61-65.
26. Mugizi, D. (2009) Relationship between bovine brucellosis and production systems in Kashongi sub-county of Kiruhura-Uganda. Bull. Anim. Health Prod. Africa. 57(3): 209-219.
27. Haileselassie, M., Kalayou, S. and Kyule, M. (2010) Serological survey of bovine brucellosis in barka and arado breeds (Bos indicus) of Western Tigray, Ethiopia. Prev. Vet. Med. 94(½): 28-35.
28. Ibrahim, N., Belihu, K., Lobago, F. and Bekana, M. (2010) Sero-prevalence of bovine brucellosis and its risk factors in Jimma zone of Oromia Region, South-western Ethiopia. Trop. Anim. Health Prod. 42: 35–40.
29. Makita, K., Fevre, E.M., Waiswa, C., Eisler, M.C., Thrusfield, M. and Welburn, S.C. (2011) Herd prevalence of bovine brucellosis and analysis of risk factors in cattle in urban and peri-urban areas of the Kampala economic zone, Uganda. BMC Vet. Res. 7: 60-63.
PMid:22004574 PMCid:PMC3212899
30. Medeiros, M.A.B., Nascif, Junior I.A. and Mathias, L.A. (2011) Prevalence of bovine brucellosis among milk suppliers of a dairy industry in Itirapua, Sao Paulo, Brazil. Ars Vet. 27(3): 152-160.
31. Calistri, P., Iannetti, S., Atzeni, M., Bella, C., Schembri, and Giovannini, A. (2013) Risk factors for the persistence of bovine brucellosis in Sicily from 2008 to 2010. Prev. Vet. Med. 110(3–4): 329–334.
32. Lindahl, E., Sattorov, N., Boqvist, S., Sattori, I. and Magnusson, U. (2014) Seropositivity and risk factors for Brucella in dairy cows in urban and peri-urban small-scale farming in Tajikistan. Trop. Anim. Health Prod. 46:563–569 .
PMid:24414248 PMCid:PMC3936117
33. Kebede, T., Ejeta, G. and Ameni, G. (2008) Seroprevalence of bovine brucellosis in smallholder farms in central Ethiopia (Wuchale-Jida district). Rev. Med. Vet. 159(1): 3-9.
34. Tolosa, T., Bezabih, D. and Regassa, F. (2010) Study on seroprevalence of bovine brucellosis and abortion and associated risk factor. Bull. Anim. Health Prod. Africa. 58(3): 239-244.
35. Chand, P. and Chhabra, R. (2013) Herd and individual animal prevalence of bovine brucellosis with associated risk factors on dairy farms in Haryana and Punjab in India. Trop. Anim. Health Prod. 45(6): 1313-1319.
36. Chatterjee, A., De, B.N., Bidyanta, J., Chakraborty, M., Mondal, P. and Sen, G.P. (1985) Sero-epidemiological studies on bovine brucellosis in organized herds in West Bengal. Indian J Anim. Sci. 55(4): 249-252.
37. Sarumathi, C., Reddy, T.V. and Sreedevi, B. (2003) Serological survey of bovine brucellosis in Andhra Pradesh. Indian J. Dairy Sci. 56: 408-410.
38. Bakhtullah, F.P., Muhammad, S., Basit, A., Khan M. A., Gul, S., Wazir, I. and Raqeebullah, K.K. (2014) Sero–Prevalence of Brucellosis in Cattle in Southern Area of Khyber Pakhtunkhwa, Pakistan. Res. J. Vet. Pract. 2(4): 63–66.
39. Swai, E.S., Mshanga, D., Sanka, N.P. and Marandu, N.H. (2003) Prevalence of bovine brucellosis in smallholder dairying farming area, Moshi, Tanzania. Trop. Anim. Health Prod. 35: 120-21.
40. Swai, E.S., Mshanga, D., Sanka, N.P. and Marandu, N.H. (2005) Bull. Anim. Health Prod. Africa. 53(2): 97-105.
41. Tesfaye, M.Y., Degefu, M.H., Tadele, T. and Woyesa, M. (2012) Bovine brucellosis: serological survey in Guto-Gida district, East Wollega zone, Ethiopia. Global Veterinaria. 8(2): 139-143.
42. Karimuribo, E., Ngowi, D., Swai, H.A.E.S. and Kambarage, D.M. (2007) Prevalence of brucellosis in crossbred and indigenous cattle in Tanzania. Livestock Research for Rural Development. 19(10): Article #148. Accessed on 02-05-2014.
43. Tebug, S.F. (2013) Factors associated with milk producer's awareness and practices in relation to zoonoses in northern Malawi. Vet World., 6(5): 249-253.
44. Azevedo, S.S., Ferreira, J.S., Neto, J.S., Ferreira, F., Dias, R.A., Amaku, M. and Vasconcellos, S.A. (2011) Association between brucellosis and occurrence of abortions in bovine from the Espirito Santo State, Southeast region of Brazil. Brazilian J. Vet. Res. Anim. Sci. 48(3): 215-219.
45. Muma, J.B., Pandey, G.S., Munyeme, M., Mumba, C., Mkandawire, E. and Chimana, H.M. (2012) Brucellosis among smallholder cattle farmers in Zambia. Trop. Anim. Health Prod. 44: 915–920.
46. Gwida, M., Al Dahouk, S., Melzer, F., Rösler, U., Neubauer, H. and Tomaso, H. (2010) Brucellosis – Regionally Emerging Zoonotic Disease? Croat. Med. J. 51: 289-95.
PMid:20718081 PMCid:PMC2931433
47. Dias, J.A., Müller, E.E., Dias, R.A., Freitas, J.C., Amaku, M., Ferreira, F., Silva, M.C.P., Lôbo, J.R., Figueiredo, V.C.F., Gonçalves, V.S.P. and Ferreira Neto, J.S. (2009) Epidemiological situation of bovine brucellosis in the state of Paraná, Brazil. Arq. Bras. Med. Vet. Zootec. 61(1): 118-21.
48. Oliveira, R.M., Silva, M.L.C.R., Macêdo, M.M.S., Higino, S.S., Paulin, L.M., Alves, C.J., Carvalho, M.G. and Azevedo, S.S. (2013) Seroepidemiology of bovine leptospirosis and brucellosis in family farm rural properties in the State of Paraíba, northeastern Brazil. Arq. Inst. Biol. 80(3): 303- 311.
49. Evangelista, L.S.M. and Goncalves, L.M.F. (2009) Bovine brucellosis in the state of Roraima. PUBVET. 3: 2 Accessed 28-08-2013.
50. Sikder, S., AKMA Rahman, Faruque, M.R., Alim, M.A., Das, S., Gupta, A.D., Das, B.C., Uddin, M.I. and Prodhan, M. (2012) Bovine brucellosis: an epidemiological study at Chittagong, Bangladesh. Pak. Vet. J. 32(4): 499-502.
51. Adesokan, H.K., Alabi, P.I., Stack, J.A. and Cadmus, S.I.B. (2013) Knowledge and practices related to bovine brucellosis transmission amongst livestock workers in Yewa, south-western Nigeria. J. S. Afr. Vet. Assoc. 84(1): 121-125.
52. Rahman, M.S., Faruk, M.O., Her, M., Kim, J.Y., Kang, S.I. and Jung, S.C. (2011) Prevalence of brucellosis in ruminants in Bangladesh. Vet. Medicina. 56(8): 379–385.
53. Tesfaye, G., Tsegaye, W., Chanie, M. and Abinet, F. (2011) Seroprevalence and associated risk factors of bovine brucellosis in Addis Ababa dairy farms. Trop. Anim. Health Prod. 43(5): 1001-1005.
54. Rahman, M.S., Her, M., Kim, J.Y., Kang, S., Lee, K., Uddin, M.J., Chakrabartty, A. and Jung, S.C. (2012) Brucellosis among ruminants in some districts of Bangladesh using four conventional serological assays. Afr. J. Microbiol. Res. 6(22):4775-4781.
55. Yoon, N.H., Cheo, H.K., Hee, M.S.J., Kim, S.L., Byeong, Y.P., Choi Kyu, J.J. and Wee, S.H. (2012) Impact of bovine brucellosis eradication programs in the Republic of Korea. Korean J. Vet. Res. 52(1): 19-24.
56. Isloor, S., Renukaradhya, G.J. and Rajasekhar, M.A. (1998) Serological survey of bovine brucellosis in India. Rev. Sci. Tech. 17: 781-785.
57. Jeyaprakash, C., Ranjitsingh, A.J.A. and Amuthan, A. (1999) Isolation of Brucella spp. from indigenous and cross-bred cows and evaluation of their antibiogram. Indian J. Anim. Res. 33: 99-103.
58. Panchasara, H. J. (2007) Sero-epizootiology, experimental modulation of vaccinal immunity, and economic impact assessment of bovine brucellosis in North Gujarat. Ph.D. thesis submitted to Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India. p143.
59. Tedele, T., Mulualem, A. and Gebreyesus, M. (2010) Sero- epidemiological survey of bovine brucellosis and reproductive health problems in North Gondar zone milkshed areas, north western Ethiopia. Bull. Anim. Health Prod. Africa. 58(2): 133-140.
60. Matope, G., Bhebhe, E., Muma, J.B., Oloya, J., Madekurozwa, R.L., Lund, A. and Skjerve, E. (2011) Seroprevalence of brucellosis and its associated risk factors in cattle from smallholder dairy farms in Zimbabwe. Trop. Anim. Health Prod. 43(5): 975–982.