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Research
(Published
online: 27-07-2014)
17. Evaluation of anthelmintic activity of Nigerian
ethnoveterinary plants Cassia occidentalis and Guiera senegalensis
- Mohammed Musa
Suleiman, Mohammed Mamman, Adamu Sidiama, Eserohene Jane Ighoja,
Mohammad Tauheed and Ahmad Muhammad Talba
Veterinary World, 7(7): 536-541
doi:
10.14202/vetworld.2014.536-541
Mohammed Musa Suleiman: Department of Pharmacology and
Toxicology, Faculty of Veterinary Medicine, Ahmadu Bello
University, Zaria, Nigeria; mohsulai@yahoo.com
Mohammed Mamman: Department of Pharmacology and Toxicology,
Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria,
Nigeria; mammanm@gmail.com
Adamu Sidiama: Department of Pharmacology and Toxicology,
Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria,
Nigeria; asidiama@yahoo.com
Eserohene Jane Ighoja: Department of Pharmacology and
Toxicology, Faculty of Veterinary Medicine, Ahmadu Bello
University, Zaria, Nigeria;
asidiama@yahoo.com
Mohammad Tauheed: Department of Pharmacology and Toxicology,
Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria,
Nigeria; mtauheed40@yahoo.com
Ahmad Muhammad Talba: Veterinary Teaching Hospital, Faculty of
Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria;
ahmadmuhammadtalba@yahoo.com
Received: 11-04-2014, Revised: 13-06-2014, Accepted: 20-06-2014,
Published online: 27-07-2014
Corresponding author: Mohammed Musa Suleiman, email:
mohsulai@yahoo.com
Abstract
Aim: This study was designed
to evaluate the anthelmintic activity of the stem-barks of Cassia
occidentalis and Guiera senegalensis which are used traditionally
to treat gastrointestinal helminth infections in goat.
Materials and Methods: Phytochemical analysis using
standard techniques was used to detect secondary metabolites
contained in the plants. In vitro anthelmintic activity of the
crude methanol extracts of the plants was determined using egg
hatch inhibition test (EHIT) and larval development inhibition
assay (LDIA).
Results: The extracts of C. occidentalis and G.
senegalensis inhibited hatching of eggs and larval development of
Haemonchus contortus in a concentration-dependent manner. At
concentrations of 0.1, 1, 10 and 100 mg/ml, the extract of C.
occidentalis produced significant (p < 0.05) inhibition of egg
hatching of H. contortus eggs when compared with the untreated
(distilled water) control group. C. occidentalis inhibited, by up
to 86%, the larval development of H. contortus. Similarly, the
extract of G. senegalensis at concentrations of 10 and 100 mg/ml
produced significant (p < 0.05) inhibition of egg hatching of H.
contortus eggs and inhibited larval development by up to 85% of
the parasite. C. occidentalis (EC50 = 4.23 mg/ml) was found to be
more efficacious than G. senegalensis (EC50 = 88.24 mg/ml) against
H. contortus eggs. However, G. senegalensis (EC50 = 0.0012 mg/ml)
was more effective than C. occidentalis (EC50 = 0.11 mg/ml)
against the larvae of H. contortus.
Conclusion: The crude methanol extracts of C. occidentalis
and G. senegalensis possess in vitro anthelmintic activity against
H. contortus that requires detailed in vivo pharmacological and
toxicological trials to justify their use in clinical veterinary
practice.
Keywords: anthelmintic, Cassia occidentalis, Guiera
senegalensis, Haemonchus contortus, medicinal plants.
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