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Research
(Published
online: 31-07-2014)
18. The comparative analysis of infection pattern and oocyst
output in Eimeria tenella, E. maxima and E. acervulina in young
broiler chicken
- Myung-Jo You
Veterinary World, 7(7): 542-547
doi:
10.14202/vetworld.2014.542-547
Myung-Jo You: Laboratory of Veterinary Parasitology, College
of Veterinary Medicine and Bio-safety Research Centre, Chonbuk
National University, Jeonju 561-756, Republic of Korea; tick@chonbuk.ac.kr,
Phone no. +82 63 270 3887, Fax. +82 63 270 3780.
Received: 25-04-2014, Revised: 21-06-2014, Accepted: 27-06-2014,
Published online: 31-07-2014
Corresponding author: Myung-Jo You, email: tick@chonbuk.ac.kr
Abstract
Aim: To assess the pattern of
infection pattern by artificially infected Eimeria strains of
Korean isolates and assessed the degree of sporulating oocysts at
different temperature.
Materials and Methods: Birds were orally inoculated with
oocysts of Eimeria tenella, Eimeria maxima and Eimeri acervulina.
Oocyst count, oocyst isolation and sporulation were evaluated from
the fourth to the tenth day post infection. Histopathological
studies also made in the caecum and intestinal to comparative
lesions.
Results: Mean oocyst counts of these species increased more
quickly on the day of 5th to 6th of post-infection. E. acervulina
reached its highest infection level on the 6th day while both E.
tenella and E. maxima had peak on the 7th day. The prepatent
period of oocyst output in the E. acervulina started 120 h and
ended on the 10th day after inoculation, whereas the E. maxima
started oocyst output at 144 h and E. tenella oocyst output at 168
h respectively. The best temperature for optimum sporulation was
found to be at 250C
at sporulation rate of 88.91%, while at 200C sporulated 88.03%,
and at 300C
sporulated 82.44%.
Conclusion: Current study represent the pattern of infection,
pathogenesis and optimum sporulation temperature and our results
suggested that 250C
is optimum for sporulated oocyst of Korean isolates of E. tenella,
E. maxima and E. acervulina.
Keywords: broiler chick, Eimeria, oocyst, pathogen.
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