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Research
(Published
online: 11-07-2014)
7. Microbial quality of culled chicken
layers in Penang, Malaysia - Ong Pek Geck, Frederick
Adzitey, Raja Arief Deli, Nurul Huda and Gulam Rusul Rahmat Ali
Veterinary World, 7(7): 478-482
doi:
10.14202/vetworld.2014.478-482
Ong Pek Geck:
Food Technology Programme, School of Industrial Technology,
Universiti Sains Malaysia, USM 11800, Penang, Malaysia; peggygeck@hotmail.com
Frederick Adzitey: Food Technology Programme, School
of Industrial Technology, Universiti Sains Malaysia, USM 11800,
Penang, Malaysia; Animal Science Department, Faculty of
Agriculture, University for Development Studies, Box TL 1882,
Tamale, Ghana; adzitey@yahoo.co.uk
Raja Arief Deli: Food Technology Programme, School of
Industrial Technology, Universiti Sains Malaysia, USM 11800,
Penang, Malaysia; deckwan@gmail.com
Nurul Huda: Food Technology Programme, School of Industrial
Technology, Universiti Sains Malaysia, USM 11800, Penang,
Malaysia; nrlhd@usm.my
Gulam Rusul Rahmat Ali: Food Technology Programme,
School of Industrial Technology, Universiti Sains Malaysia, USM
11800, Penang, Malaysia; gulam@usm.my
Received: 02-04-2014, Revised: 05-05-2014, Accepted: 05-06-2014,
Published online: 11-07-2014
Corresponding author: Gulam Rusul Rahmat Ali, email:
gulam@usm.my, Tel: +60122103046 Fax: +6046573678.
Abstract
Aim: To determine the
microbial quality of culled chicken layers in Penang, Malaysia.
Materials and Methods: Samples were obtained from three
layer farms (designated as Farm A, Farm B and Farm C). A total of
67 culled chicken layer samples consisting of egg wash water,
chicken carcass rinse, drinking water, cloaca swab, feed and
faeces were examined for enterobacteriaceae, total and faecal
coliforms, and Escherichia coli using the procedures in the
bacteriological analytical manual.
Results: The total plate count for bacteria ranged from 2.7
x 103 cfuml-1 to 1.8 x 107 cfug-1 (Farm A), <1.0 x 102 cfuml-1 to
1.7 x 106 cfug-1 (Farm B) and <1.0 x 102 cfuml-1 to 3.1 x 106
cfug-1 (Farm C). Enterobacteriaceae count ranged from <1.0 x 102
cfuml-1 to 3.5 x 106 cfug-1 (Farm A), <1.0 x 102 cfuml-1/cfug-1 to
1.2 x 106 cfug-1 (Farm B) and <1.0 x 102 cfuml-1/cfug-1 to 2.8 x
106 cfug-1 (Farm C). Total and faecal coliforms ranged from <3.0
to >1100.0 Most probable number (MPN)ml-1/ MPNg-1 for Farm A, B
and C. Similarly, E. coli count for all the three farms ranged
from <3.0 to >1100.0 MPNml-1/MPNg-1. E. coli counts were very low
for most of the samples examined except chicken carcass and faeces.
Conclusion: In general, Farm A had higher bacterial count,
followed by Farm C and Farm B. This work gives an indication that
pathogenic foodborne pathogens such as E. coli, Salmonella,
Citrobacter, Enterobacter, Klebsiella, Shigella and Yersinia spp.
may be present in culled chicken layers and consequently pose the
risk of causing food poisoning or outbreaks.
Keywords: coliforms, culled chicken layers, E. coli,
enterobacteriaceae, total plate count.
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