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Research
(Published
online: 14-07-2014)
8. Sub-clinical mastitis prevalent in dairy cows in Chittagong
district of Bangladesh: detection by different screening tests
- Mukti Barua, Mohammad Abdul Matin Prodhan, Kamrul Islam,
Sharmin Chowdhury, Md. Hasanuzzaman, Mohammed Ashif Imtiaz and
Goutam Buddha Das
Veterinary World, 7(7): 483-488
doi:
10.14202/vetworld.2014.483-488
Mukti Barua:
Department of Animal Science and Nutrition, Chittagong Veterinary
and Animal Sciences University, Khulshi, Chittagong-4202,
Bangladesh; mukti.barua@yahoo.com
Mohammad Abdul Matin Prodhan:
Department of Medicine and Surgery, Faculty of Veterinary
Medicine, Chittagong Veterinary and Animal Sciences University,
Khulshi, Chittagong-4202, Bangladesh; cvasu.prodhan@rocketmail.com
Kamrul Islam:
Department of Microbiology, Faculty of Veterinary Medicine,
Chittagong Veterinary and Animal Sciences University, Khulshi,
Chittagong-4202, Bangladesh; kamruldvm13@gmail.com
Sharmin Chowdhury:
Department of Pathology and Parasitology, Faculty of Veterinary
Medicine, Chittagong Veterinary and Animal Sciences University,
Khulshi, Chittagong-4202, Bangladesh; sharminchowdhury@ygmail.com
Md. Hasanuzzaman:
Department of Animal Science and Nutrition, Chittagong Veterinary
and Animal Sciences University, Khulshi, Chittagong-4202,
Bangladesh;
anikzaman@yahoo.com
Mohammed Ashif Imtiaz:
Department of Physiology, Biochemistry and Pharmacology; Faculty
of Veterinary Medicine, Chittagong Veterinary and Animal Sciences
University, Khulshi, Chittagong-4202, Bangladesh; ashif.shawn@gmail.com
Goutam Buddha Das:
Department of Animal Science and Nutrition, Chittagong Veterinary
and Animal Sciences University, Khulshi, Chittagong-4202,
Bangladesh; GBD: gbmadhu07@yahoo.com
Received: 09-04-2014, Revised: 03-05-2014, Accepted: 05-06-2014,
Published online: 14-07-2014
Corresponding author: Mukti Barua, email: mukti.barua@yahoo.com,
Cell: +88-01846335679
Abstract
Aim: Mastitis is recognized
as one of the most costly health disorder affecting dairy cows. An
epidemiological study was carried out at some selected farms in
Chittagong district of Bangladesh to determine the prevalence and
risk factors of sub-clinical mastitis (SCM) in dairy cows.
Materials and Methods: For conducting the study, some dairy
farms of Chittagong were selected from urban and periurban areas
by stratified random sampling. A total of 444 quarter samples of
111 (56 from commercial dairy farms and 55 from backyards)
lactating dairy cows were considered. Sub-clinical mastitis (SCM)
was determined using three different indirect screening tests:
California Mastitis Test (CMT), White Slide Test (WST) and Surf
Field Mastitis Test (SFMT). Sensitivity and specificity were also
determined to measure the accuracy of those tests.
Results: The prevalence of SCM by CMT, WST and SFMT were
32.43% (n=144), 33.56% (n=149) and 31.53% (n=140), respectively.
Distribution of SCM in relation to different variables at quarter
level and animal level was also recorded. The prevalence of SCM
was significantly (P<0.05) higher in aged, high yielding cows in
addition with history of periparturient diseases, without dry cow
therapy both at quarter and animal level. A significantly (p<0.01)
higher prevalence (48.98%) of SCM was observed in higher parity
number (>4) than others at quarter level. No significant
difference (P>0.05) was found in relation to breed. Using CMT as a
gold standard, sensitivity and specificity of WST and SFMT were
also calculated at 95% confidence interval. The sensitivity,
specificity, positive likelihood ratio, negative likelihood ratio,
positive predictive value, negative predictive value and disease
prevalence by WST and SFMT were comparable.
Conclusion: This study recommends that regular screening of
sub-clinical mastitis will reduce the prevalence of sub-clinical
mastitis. The most effective way to control sub-clinical mastitis
is to take preventive measures such as regular cleaning of the
floor, keeping the udder clean, milkman's cleanliness, dry cow
therapy specially in high yielding dairy cows.
Keywords: CMT, sensitivity, SFMT, specificity, sub-clinical
mastitis, WST.
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