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Research
(Published
online: 04-06-2014)
1. Detection and molecular
characterization of Shiga toxin producing Escherichia coli (STEC)
autoagglutinating adhesion gene (saa) from piglets in Mizoram
- J. L. Kataria, T. K. Dutta, P. Roychoudhury and J. G. Tiwari
Veterinary World, 7(6): 373-376
doi:
10.14202/vetworld.2014.373-376
J. L. Kataria: College of Veterinary Sciences and
Animal Husbandry, Central Agricultural University, Aizawl, Mizoram-
796014, India;
jluvjkat@gmail.com
T. K. Dutta: College of Veterinary Sciences and Animal
Husbandry, Central Agricultural University, Aizawl, Mizoram-
796014, India; tapandutta@rediffmail.com
P. Roychoudhury: College of Veterinary Sciences and Animal
Husbandry, Central Agricultural University, Aizawl, Mizoram-
796014, India;
parimal74@rediffmail.com
J. G. Tiwari: College of Veterinary Sciences and Animal
Husbandry, Central Agricultural University, Aizawl, Mizoram-
796014, India;
tapandutta72@yahoo.com
Received: 02-04-2014, Revised: 01-05-2014, Accepted: 06-05-2014,
Published online: 04-06-2014
Corresponding author: T. K. Dutta, email: tapandutta@rediffmail.com
Abstract
Aim: The study was carried
out to detect and characterize the Shiga toxin producing
Escherichia coli (STEC) auto aggluti-nating adhesion gene (saa)
from Piglets in Mizoram.
Materials and Methods: A total 100 fecal samples from 0–3
month old piglets with (60) or without (40) diarrhoea were
collected from different parts of Mizoram and screened for the
presence of E. coli. All the locus of enterocyte (LEE) negative
STEC strains was tested for the presence saa gene by polymerase
chain reaction (PCR).
Results: In this study, a total of 254 E. coli isolated and
identified. Altogether, 51 E. coli were found to be positive for
at least one virulence gene tested, of which 30 and 21 were
classified as STEC and EPEC, respectively. A total of 4 E. coli
isolates were found to be positive for saa gene, of which one was
from diarrhoeic and three were from healthy piglets.
Conclusions: STEC and EPEC are prevalent in pig population
of Mizoram. Presence of saa gene positive E. coli in pigs in this
region may pose a threat to public health.
Keywords: locus for enterocyte effacement, STEC
autoagglutinating adhesion, Shigatoxigenic Escherichia coli , STEC.
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