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Research (Published online: 11-06-2014)

6. Evaluation of immunochromatographic assay for serodiagnosis of bovine brucellosis in Gezira State, Sudan - A. M. El-Eragi, Manal H. Salih, Mihad F. E. M. Alawad and K. B. Mohammed

Veterinary World, 7(6): 395-397



   doi: 10.14202/vetworld.2014.395-397



A. M. El-Eragi: Department of Pathology and Diagnosis, Veterinary Research Institute. P. O. Box 8067 (ALAmart), Khartoum, Sudan;

Manal H. Salih: Department of Pathology and Diagnosis, Veterinary Research Institute. P. O. Box 8067 (ALAmart), Khartoum, Sudan;

Mihad F. E. M. Alawad: Department of Brucella, Veterinary Research Institute. P. O. Box 8067 (ALAmart), Khartoum, Sudan;

K. B. Mohammed: Department of Pathology and Diagnosis, Veterinary Research Institute. P. O. Box 8067 (ALAmart), Khartoum, Sudan;

Received: 15-02-2014, Revised: 02-05-2014, Accepted: 08-05-2014, Published online: 11-06-2014


Corresponding author: K. B. Mohammed, email:

1. Baek, B.K., Lim, C.W., Rahman, M.S.C., Kim, H., Oluoch, A. and Kakoma, I. (2003) Brucella abortus infection in indigenous Korean dogs. Can. J. Vet. Res., 67: 312-314.
PMid:14620870 PMCid:PMC280718
2. Kakoma, I., Oluoch, A.O., Baek, B.K., Rahman, M.S and Matsuda, K. (2003). More attention warranted on Brucella abortus in animals. J. Am. Vet. Med. Assoc., 222: 284.
3. WHO (1986) Joint FAO/WHO Expert Committee on Brucellosis. World Health Organ Tech Rep Ser., 740:1-132.
4. Franco, M.P., Mulder, M., Gilman, R.H. and Smits, H.L. (2007) Human brucellosis. Lancet Infect. Dis.,7: 775-786.
5. Godfroid, J., Cloeckaert, A., Liautard, J.P., Kohler, S., Fretin, D., Walravens, K, Garin Bastuji, B. and Letesson, J.J. (2005) From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a reaemerging zoonosis. Vet. Res., 36: 313-326.
6. Pappas, G., Papadimitriou, P., Akritidis, N., Christou, L. and Tsianos, E.V. (2006) The new global map of human brucellosis. Lancet Infect. Dis., 6: 91-99.
7. Smits, H.L. and Kadri, S.M. (2005) Brucellosis in India: a deceptive infectious disease. Indian J. Med. Res.,122: 375- 384.
8. Fernando, P.P., Klaus, N., Luis, E.S. and Wei, L.Y. (2010) Diagnosis of brucellosis. Open Vet. Sci. J., 4: 46-60.
9. Office International des Epizooties (OIE) (2009) OIE Terrestrial Manual, Chapter 2.4.3., Bovine brucellosis. BOVINE _BRUCELL. Pdf. Accessed on 07-06-2017.
10. Jabbar, A.A., AL-Sa'aidi, M.A. and AL-Rodh, A.A.N. (2012) Clinical, Serological, Hormonal, Bacteriological and Molecular Detection of Brucellosois in Aborted Cows and Buffals. Int. Conference on Applied Life Science, Turkey.
11. Nielsen, K., Gall, D.P., Smith, S., Balsevicius and Garrido, F. (2004) Comparison of serological tests for the detection of ovine and caprine antibody to Brucella melitensis. Rev. Sci. Tech., 23: 979-987.
12. Lou, S.C., Patel, C., Ching, S. and Gordon, J.(1993) One- step competitive immunochromatographic assay for semiquantitative determination of lipoprotein (a) in plasma. Clin. Chem., 39: 619-624.
13. Birnbaum, S., Uden, C., Magnusson and Nilsson, S. (1992) Latex-based thin-layer immunoffmity chromatography for quntitation of protein analytes. Anal. Biochem., 206: 168-171.
14. Abdoel, T.H. and Smith, H.L. (2007). Rapid latex agglutination test for serodiagnosis of human brucellosis. Diagn. Microbiol. Infect. Dis., 57(2):123-128.
15. Diaz-Aparicio, E., Aragon, V., Marin, C., Alonso, B., Font, M., Moreno, E., Perez-Ortiz, S., Blasco, J.M., Diaz, R. and Moriyon, I. (1993) Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica 0:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep and goats. J. Clin. Microbiol., 31: 3136-3141.
PMid:8308104 PMCid:PMC266364
16. Hilbink, F., Fenwick, S.G., Thompson, E.J., Ittelberger, R.K., Penrose, M., and Ross, G.P. (1995) Non-specific seroreactions against Brucella abortus in ruminants in New Zealand and the presence of Yersinia enterocolitica 0:9, NZ. Vet. J., 3(43): 175-178.
17. Weynants, V., Tibor, A., Denoel, P.A., Saegerman, C. and Godfroid, J. (1996) Infection of cattle with Yersinia enterocolitica 0:9 cause of false positive serological reaction in bovine brucellosis diagnostic test. Vet. Microbiol., 48: 101-112.
18. Bronsvoort, B.M., Ele, C., Koterwas, B., Land, F., Handel, I.G. and Tucker, J. (2009) Comparison of a Flow Assay for Brucellosis Antibodies with the reference'' cELISA'' test in West African Bos indicus. Plos One., 4(4): 5221.
PMid:19381332 PMCid:PMC2667634
19. Radulescu, R.A., Petriceanu, G., Ragalie, A. and Gutu, E. (2007) Comparative evaluation of serological assays for brucellosis diagnosis. Rev. Rom. Med. Vet., 17: 83-92.
20. Theresia, A., Isabel, T.D., Regina, C. and Henk, L.S. (2008) Simple and rapid field tests for brucellosis in livestock. Vet. Microbiol., 130: 312-319.
21. Blasco, J.M., Garin-Bastuji, B., Marin, C.M., Gerbier, G., Fanlo, J., Jimenez de Bagues, M.P. and Cau, C. (1994) Efficacy of different Rose Bengal and complement fixation antigens for the diagnosis of Brucella Melitensis infection in sheep and goats. Vet. Rec., 134: 415-420.
22. Horie, M., Ogaawa, H., Yamada, K., Hara, H., Bui, V.N., Sanaa, S., Awad., Yoshikawa, R., Tusukamato, K., Yamaguchi, S., Nakamura, K. and Imai, K. (2009) Alatex agglutination test using a recombinant nucleoprotein for detection of antibodies against avian influenza virus. J. Virol. Methods, 161(2): 259-264.
23. Junxing Yang, Qunyi Hua, Huanchun Chen, Jianqiang Lv, Bing Chen and Zhouxi Ruan, (2010) Arapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen. J. Vet Diagn Invest., 22(2): 242-4.
24. Tma Senthilkumar, M. Subathra, M. Phil, P. Ramadass and V. Ramaswamy, (2009) Rapid serodiagnosis of leptospirosis by latex agglutination test and flow - through assay. Indian J. Med. Microbiol., 26(1): 45-49.