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Research
(Published
online: 22-03-2014)
11. Prevalence of theileriosis in
cross-bred cattle: its detection through blood smear examination
and polymerase chain reaction in Dehradun district, Uttarakhand,
India - Shweta Kohli, U. K. Atheya and A. Thapliyal
Veterinary World, 7(3): 168-171
doi:
10.14202/vetworld.2014.168-171
Abstract
Aim: Theileriosis is a major
threat to dairy and related industries. Theileria is a protozoan
parasite that causes theileriosis, a fatal disease. The aim of the
present study was to assess the prevalence of theileriosis in
crossbred cattle belonging to Dehradun district, Uttarakhand,
India.
Materials and Methods: We screened 301 blood samples each
month from apparently normal/healthy crossbred cattle from various
locations of Dehradun district. Samples were tested using Giemsa's
staining technique and specific PCR test.
Result: Microscopic examination of blood smears revealed
27.2% (82) overall prevalence of theileriosis. The highest
prevalence was found in rainy season with a prevalence rate 45.4%.
By Theileria genus specific PCR test, 98 samples (32.5 %) were
found to be positive for theileriosis.
Conclusion: Our study suggests that theileriosis is
spreading to even the temperate region in Uttarakhand and
immediate screening norms/policies are needed to reduce the extent
of spread. There is a need for further investigation using
molecular technique.
Keywords: blood smear, PCR, seasonal prevalence, Theileria,
Uttarakhand.
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