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Research (Published online: 22-05-2014)

14. Sequencing and phylogenetic analysis of partial CXCR2 gene of Murrah buffalo - S. A. Wani, M. L. Sangwan, M. A. Dar, A. Kumar, M. A. Rafee and D. Baro
Veterinary World, 7(5): 342-346

 

 

   doi: 10.14202/vetworld.2014.342-346

 


 S. A. Wani: Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar - 243122, Uttar Pradesh, India

M. L. Sangwan: Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Science, Hisar, Haryana, India

M. A. Dar: Department of Veterinary Physiology and Biochemistry, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbel, Bangalore, Karnataka, India

A. Kumar: Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Science, Hisar, Haryana, India

M. A. Rafee: Division of Veterinary Surgery and Radiology, Indian Veterinary Research Institute, Izatnagar - 243122, Uttar Pradesh, India

D. Baro: Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Science, Hisar, Haryana, India


Received: 22-02-2014, Revised: 07-04-2014, Accepted: 16-04-2014, Published online: 22-05-2014


Corresponding author: S. A. Wani, email: wanisajad759@gmail.com

Abstract

Aim: Present study was carried out to sequence and phylogenetic analysis of CXCR2 gene of Murrah buffalo.
Materials and Methods: For the present investigation, from a group of forty eight Murrah buffaloes (Bubalus bubalis), blood samples were collected randomly from eight animals, out of which four were healthy and four were mastitic.
Results: The amplification of Interleukin-8B (IL-8B) receptor gene target sequence was carried out using the primer pair in an optimized polymerase chain reaction. Partial sequencing of IL-8B receptor gene of Bubalus bubalis (Murrah) has been done successfully. The sequences of IL-8B receptor gene showed 99% homology to that of Bos indicus × Bos taurus, 98% to that of Bos taurus, 97% to that of Ovis aries, 93% to that of Sus scrofa, 92% to that of Equus caballus and 90% to that of Felis catus.
Conclusion: From the present study it can be concluded that the PCR amplification procedure for target region of IL-8B receptor gene yielding 459 bp products has been standardized, which yielded consistent and specific amplification. Amplification of partial IL-8B receptor gene (exon 2- 459 bp) using self designed primers specific for cattle ortholog sequence signifies that the locus is conserved in cattle and buffaloes. In phylogenetic tree, the target sequence of IL-8B receptor gene of Bubalus bubalis were found to be more closely related to Bos indicus × Bos Taurus and Bos taurus than to Ovis aries and Sus scrofa.
Keywords: CXCR2 gene, Murrah buffalo, phylogenetic analysis.

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