Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916

ISSN (Print): 0972-8988


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online: 25-05-2014)

16. Molecular diagnosis and adaptation of highly virulent infectious bursal disease virus on chicken embryo fibroblast cell - Yogender Singh, Kalpana Yadav, Vinod Kumar Singh and Rakesh Kumar
Veterinary World, 7(5): 351-355



   doi: 10.14202/vetworld.2014.351-355


Yogender Singh:
Animal Biotechnology Centre, Nanaji Deshmukh Pashu-Chikitsa Vigyan Vishwavidyalaya, Jabalpur, Madhya Pradesh - 482004, India

Kalpana Yadav: Animal Biotechnology Centre, Nanaji Deshmukh Pashu-Chikitsa Vigyan Vishwavidyalaya, Jabalpur, Madhya Pradesh - 482004, India

Vinod Kumar Singh: Division of Virology, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, India

Rakesh Kumar:  Division of Dairy Cattle and Breeding, National Dairy Research Institute, Karnal, Haryana-132001, India

Received: 13-03-2014, Revised: 13-04-2014, Accepted: 19-04-2014, Published online: 25-05-2014

Corresponding author:
Yogender Singh, email:


1. Rashid, M.H., Chunyi, X., Islam, M.T., Islam, M.R., Zheng, and Yongchang, C. (2013) Comparative epidemiological study of infectious bursal disease of commercial broiler birds in Bangladesh and China. Pak. Vet. J., 33(2): 160-164.
2. Luque, D., Saugar, I., Rejas, M.T., Carrascosa, J.L., Rodriguez, J.F. and Caston, J.R. (2009) Infectious bursal disease virus: ribonucleoprotein complexes of a double- stranded RNA virus. J. Mol. Biol.,386: 891-901.
3. Delgui, L., Ona, A., Gutierrez, S., Luque, D., Navarro, A., Caston, J.R. and Rodriguez, J.F. (2009) The capsid protein of infectious bursal disease virus contains a functional alpha 4 beta 1 integrin ligand motif. Virology, 386: 360-372.
4. Kumar, S., Ahi, Y.S., Salunkhe, S.S., Koul, M., Tiwari, A.K., Gupta, P.K. and Rai, A. (2009) Effective protection by high efficiency bicistronic DNA vaccine against infectious bursal disease virus expressing VP2 protein and chicken IL-2. Vaccine, 27: 864-869.
5. Heine, H.G., Haritou, M., Failla, P., Fahey, K. and Azad, A. (1991) Sequence analysis and expression of the host protective immunogen VP2 of a variant strain of infectious bursal disease virus which can circumbent vaccination with standard type 1 strains. J. Gen. Virol., 72: 1835-1843.
6. Olivier, E., Cyril, L.N., Michel, A., Xavier, A., Kristen, M.O., Olivier, G., Didier, T., Hermann, M., Mohammed, R. I. and Nicolas, E. (2013) Both genome segments contribute to the pathogenicity of very virulent infectious bursal disease virus. J. Virol., 87(5): 2767-2780.
PMid:23269788 PMCid:PMC3571395
7. Khan, M.A., Hussain, I., Siddique, M., Rahman, S.U. and Arshad, M. (2007) Adaptation of a local wild infectious bursal disease virus chicken embryo fibroblast cell culture. Int. J. Agri. Biol., 9: 925-927.
8. Barlic, M.D., Zorman, R.O. and Grom, J. (2002) Detection of infectious bursal disease virus in different lymphoid organs by single-step reverse transcription polymerase chain reaction and micro-plate hybridization assay. J. Vet. Diagn. Invest., 14: 243-246.
9. Sambrook, J. and Russel, D.W. (2001) Molecular Cloning: A Laboratory Manual. 3rd Edn., Cold Spring Harbor Laboratory Press, New York. p460-473.
10. Cunningham, C. H. (1973). A laboratory guide in virology. Burgess Publishing Co. Minnesota .7th Edn. p155-169.
11. Reed, L. J. and Muench, H. (1938) A simple method for estimating fifty percent end points. Ame. J. Hyg., 27: 493- 497.
12. Rathore, S., Kumar, A., Thakur, P., Kumari, N., Meena, M. and Nimje, P. (2013) Pathological Study of infectious bursal disease in broilers of Haryana. J. Immunol. Immunopathol., 15(1): 62-65.
13. Islam, K. M. D. (1999) Adaptation of reovirus on Vero cell line. B.Sc. Thesis, Biotechnology Discipline, Khulna University, Khulna, Bangladesh.
14. Engstrom, B., Eriksson, H., Fossum, O. and Jansson, D. (2003) Fjaderfasjukdomar, Sveriges Veterinärmedicinska Anstalt.
15. Sharma, J.M. (2003) Immunity and Immunosuppressive Diseases of Poultry, Convention notes American Association of Veterinary Medicine/World Veterinary Poultry Asso- ciation.
16. Tao, Q., Wang, X., Bao, H., Wu, J., Shi, L., Li, Y., Qiao, C., Yakovlevich, S.A., Mikhaylovna, P.N. and Chen, H. (2009) Detection and differentiation of four poultry diseases using asymmetric reverse transcription polymerase chain reaction in combination with oligonucleotide microarrays. J. Vet. Diagn. Invest., 21: 623-632.
17. Amara, A., Akeila, M. and Khalil, S. (2011) Detection and isolation of infectious bursal disease virus from the field. Alexandria Journal of Veterinary Sciences (AJVS), 34 (1): 185-197.
18. Barathidasan, R., Singh, S. D., Kumar, M. A., Desingu, P. A., Palanivelu, M., Singh, M. and Dhama, K. (2013) Recurrent outbreaks of infectious bursal disease (IBD) in a layer farm caused by very virulent IBD virus (vvIBDV) in India: pathology and molecular analysis. South Asian Journal of Experimental Biology (SAJEB), 3(4): 200-206.
19. Yu, F., Qi, X., Yuwen, Y., Wang, Y., Gao, H., Gao, Y., Qin, L. and Wang, X. (2010) Molecular characteristics of segment B of seven very virulent infectious bursal disease viruses isolated in China. Virus Genes 41: 246-249.
20. Hossain, S.K.A., Uddin, S.N., Rahman, M.S., Wadud, A. and Khan, M.H. (2006) Propagation of infectious bursal disease virus (IBDV) in chicken embryo fibroblast cells. J. Biol. Sci., 6: 146-149.
21. Simoni, I.C., Custodio, R.M. and Fernandes, M.J.B. (1999) Susceptibility of cell lines to avian viruses. Rev. Microbial., 30: 9-13.
22. Mohammed, M. H., Hair-Bejo, M., Omar, A. R. and Aini, I. (2012) Replication of very virulent infectious bursal disease virus in the chicken mesenchymal stem cells. J. Adv. Med. Res., 2(1): 1-7.
23. Kibenge, F. S. B., Dhillon, A.S. and Russell, R.G. (1988). Growth of serotype-I and II and variant strains of infectious bursal disease virus in Vero cells. Avian Dis.,32: 298-303.