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Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online: 29-05-2014)

18. Genotyping of the Holstein-Friesian crossbred cattle for CD18 gene using PCR-RFLP - A. S. Khade, A. Y. Doiphode, U. D. Umrikar, M. P. Sawane and V. D. Pawar
Veterinary World, 7(5): 360-362



   doi: 10.14202/vetworld.2014.360-362


A. S. Khade: 
Department of Animal Genetics and Breeding, Bombay Veterinary College, Parel, Mumbai-400012, Maharashtra, India.

A. Y. Doiphode: Department of Animal Genetics and Breeding, Bombay Veterinary College, Parel, Mumbai-400012, Maharashtra, India.
U. D. Umrikar: Department of Animal Genetics and Breeding, Bombay Veterinary College, Parel, Mumbai-400012, Maharashtra, India.
M. P. Sawane: Department of Animal Genetics and Breeding, Bombay Veterinary College, Parel, Mumbai-400012, Maharashtra, India.
V. D. Pawar: Department of Animal Genetics and Breeding, Bombay Veterinary College, Parel, Mumbai-400012, Maharashtra, India.

Received: 04-03-2014, Revised: 22-04-2014, Accepted: 25-04-2014, Published online: 29-05-2014

Corresponding author: 
A.S. Khade, email:; Tel: +91-8080449956


1. Nagahata, H. (2004) Bovine leukocyte adhesion deficiency (BLAD): A review. J. Vet. Med. Sci., 66(12): 1475-82.
2. Shuster, D.E., Kehrli, M.E., Ackerman, M.R. and Gilbert, R.O. (1992) Identification and prevalence of a genetic defect that causes leukocyte adhesion deficiency in Holstein cattle. Proc Natl Acad Sci., 89: 9225-9229.
PMid:1384046 PMCid:PMC50098
3. Pareek, S. and Kaminski, S. (1996) Bovine leukocyte adhesion deficiency (BLAD) and its worldwide prevalence. J. App. Gen., 37(3): 299-300.
4. Lubieniecki, K., Grzybowski, G., Lukaszewicz, M. and Lubieniecka, J. (1999) Association between the presence of allele BL in the genome of dairy cows and their productivity. Anim. Sci. Pap. Rep., 17: 189194.
5. Padeeri, M., Vijaykumar, K., Grupe, S., Narayan, M.P., Schwerin, M. and Kumar, H.M. (1999) Incidence of hereditary citrullinemia and bovine leucocyte adhesion deficiency syndrome in Indian dairy cattle (Bos aurus, Bos indicus) and buffalo (Bubalus bubalis) population. Arch Tierz., 42: 347352.
6. Patel, M., Patel, R.K., Singh, K.M., Rank, K.D.N., Thakur, M.C. and Khan, A. (2011) Detection of Genetic Polymorphism in CD18 Gene in Cattle by PCR-RFLP. Wayamba J. Anim. Sci., 110-111.
7. Mahdi, M., Sharma, A., Dubey, P.P., Singh, A., Tantia, M.S., Mishra, B., Kumar, V. and Joshi, B.K. (2010) PCR based identification of Bovine Leukocyte Adhesion Deficiency Syndrome (BLAD) carriers in Karan Fries bulls. Indian. J. Anim. Sci., 80(5): 433-35.
8. Agerholm, J.S., Houe, H., Jorgensen, C.B. and Basse, A. (1993) Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian cattle. II. Pathoanatomical description of affected calves. Acta.Vet. Scan., 34: 237243.
9. Czarnik, U. and Kaminski, S. (1997) Detection of intronic sequence in the bovine ITGB2 gene. J. Anim. Gen., 28(4): 32021.
10. Yathish, H.M., Sharma, A., Kumar, V., Jain, A., Chakraborty, D., Singh, A., Tantia, M. S. and Joshi, B.K. (2010) Genetic Polymorphism of CD18 gene in Karan Fries Young bull Calves using PCR-RFLP Analysis. Current Trends Biotechnol. Pharm., 4(4): 900-907.
11. Biochard, D., Coquereau, J.A. and Amigues, Y. (1995) Effect of Bovine leukocyte adhesion deficiency (BLAD) genetic defect in Holstein cattle under farm condition, 46th Ann. Mett. Eur. Assoc. Anim. Prod. Prague, 14: 197-34.
12. Poli, M.A., Dewey, R. L., Semorile, M.E., Lozano, C.G., Romanowski, V. and Grau, O. (1996) PCR Screening for carriers of Bovine Leukocyte Adhesion Deficiency (BLAD) and Uridine Monophosphate Synthase (DUMPS) in Argentine Holstein Cattle. J. Vet. Med. Series A, 43: 163168.
13. Nasreen, F., Malik, N.A., Riaz, M.N. and Qureshi, J.A. (2009) Detection and screening of bovine leukocyte adhesion deficiency in Pakistan using molecular methods. Hereditas, 146: 74-78.
14. Ribeiro, L.A., E.B. Erica, M.L. Martinez and L.L. Coutinho (2000) PCR screening and allele frequency estimation of Bovine Leukocyte Adhesion Deficiency in Holstein and Gir cattle in Brazil.Genet. Mol. Biol., 23(4).
15. Norouzy, A., Nassiry, M.R., Eftekharishahrody, F., Javadmanesh, A., Mohammad Abadi M.R. and Sulimova, G.E. (2005) Identification of Bovine Leukocyte Adhesion Deficiency (BLAD) carriers in Holstein and Brown Swiss AI Bulls in Iran, Russ. J. Genet., 41(12): 1697-1701.
16. Meydan, H.A., Mehmet, Y. and Agerholm, J.S. (2010) Screening for Bovine Leukocyte Adhesion Deficiency, Deficiency of Uridine Monophosphate Synthase, Complex Vertebral Malformation, Bovine Citrullinaemia, and Factor XI deficiency in Holstein cows reared in Turkey. Acta Vet. Scand., 52: 56.
PMid:20929557 PMCid:PMC2959049
17. Jianbin, L., Zhong, J., Wang, H., Zhang, Y. and Hou, M. (2011) Identification of BLAD and citrullinemia carriers in Chinese Holstein cattle. Anim. Sci. Pap. and Rep., 29(1): 37- 42.
18. Adamov, N., Mitrov, D., Esmerov, I. and Dovc, P. (2014) Detection of recessive mutations (BLAD AND CVM) in Holstein Friesian cattle population in Republic of Macedonia. Mac. Vet. Rev., 37 (1): 61-68.
19. Şahin, E., Taki K., Askin G. and Sonar, M. (2013) Identification of bovine leukocyte adhesion deficiency (BLAD) and bovine citrullinaemia (BC) alleles in Holstein cows reared in Antalya region. J. App. Ani. Res., 41(1). p56- 60.
20. Paiva, D.S., Fonseca, I., Pinto, I.S.B., Ianella, P., Campos, T.A., Caetano, A.R., Paiva, S.R., Silva, M.V.G.B. and Martins, M.F. (2013) Incidence of bovine leukocyte adhesion deficiency, complex vertebral malformation, and deficiency of uridine-5-monophosphate synthase carriers in Brazilian Girolando cattle. Genet. Mol. Res. 12 (3): 3186- 3192.
21. Patel, R.K., Krishna, M.S., Kalpesh, J.S., Jenabhai, B.C. and Krothapalli, R.S.S.R. (2007) Low incidence of Bovine Leukocyte Adhesion Deficiency (BLAD) carriers in Indian cattle and buffalo breeds. J. Appl. Gen., 48: 153-155.