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Research
(Published
online: 29-05-2014)
19. Isolation, characterization, antibiogram and pathology of
Pasteurella multocida isolated from pigs - Mamta Tigga,
R. C. Ghosh, Praveen Malik, B. K. Choudhary, Pratap Tigga and
Dinesh Kumar Nagar
Veterinary World, 7(5): 363-368
doi:
10.14202/vetworld.2014.363-368
Mamta Tigga:
National Research Centre on Equines, Sirsa Road, Hisar, Haryana,
India
R. C. Ghosh:
Department of Veterinary Pathology, College of Veterinary Science
& Animal Husbandry, Chhattisgarh Kamdhenu Veterinary University,
Anjora, Durg, Chhattisgarh, India
Praveen Malik:
National Research Centre on Equines, Sirsa Road, Hisar, Haryana,
India
B. K. Choudhary:
ICAR Research Complex for Eastern Region, Patna, Bihar, India
Pratap Tigga:
Department of Veterinary Pathology, College of Veterinary Science
& Animal Husbandry, Chhattisgarh Kamdhenu Veterinary University,
Anjora, Durg, Chhattisgarh, India
Dinesh Kumar Nagar:
National Research Centre on Equines, Sirsa Road, Hisar, Haryana,
India
Received: 26-03-2014, Revised: 22-04-2014, Accepted: 28-04-2014,
Published online: 29-05-2014
Corresponding author:
Mamta Tigga, email: chiyamum@gmail.com
Abstract
Aim: Isolation,
characterization and antibiogram of Pasteurella multocida from
diseased pigs of district Durg of Chhattisgarh, and to study
pathological changes caused by swine pasteurellosis.
Materials and Methods: An outbreak of swine pasteurellosis
was suspected in pigs of Ruwabandha (Bhilai), Anjora, Somni,
Tedesara, Tirgajhola villages of Durg district in Chhattisgarh,
India during August and September of 2011. Nasal Swabs and blood
samples from ailing pigs and heart blood and impression smears
from morbid pigs were processed for detection and isolation of P.
multocida by bacteriological methods. Detailed necropsy was
conducted and gross and histopathological lesions were recorded.
The test Isolates were subjected to antimicrobial sensitivity
profile by disc-diffusion method.
Results: The blood smears from heart blood and tissue
impression smears revealed teaming of bipolar organisms indicating
the presence of Pasteurella spp. The isolates obtained were
subjected to Gram's staining for checking the purity and bipolar
morphology and characterized biochemically. Gross lesions included
severe acute pneumonia and haemorrhages in lungs, petechial
haemorrhages on serous membranes and other visceral organs. On
histopathological examination, lungs showed typical fibrinous
bronchopneumonia, multifocal suppuration. All the isolates of P.
multocida were 100% sensitive to Amoxicillin, Gentamicin,
Enrofloxacin and showed100% resistance to Ceftizoxim and
Cloxacillin.
Conclusion: Gross and microscopic lesions in dead animals
are of great diagnostic value and are of characteristic of P.
multocida infection. Cultural, morphological and biochemical
characters are useful to rule out the causative agent as P.
multocida. Antibiotic sensitivity pattern of the isolates should
routinely be carried out for knowing the antibiotic resistance
trends in an endemic area.
Keywords: Chhattisgarh, Pasteurella multocida, pathology,
pneumonic pasteurellosis, swine pasteurellosis.
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