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Research
(Published
online: 15-05-2014)
9. Effect of harvesting technique and
presence or absence of corpus luteum on in vitro development after
parthenogenetic activation of oocytes recovered from buffalo
ovaries
- Yelisetti Uma Mahesh, Murakonda Mutha Rao, Poda
Sudhakar and Krothapalli Raja Surya Sambasiva Rao
Veterinary World, 7(5): 315-320
doi:
10.14202/vetworld.2014.315-320
Yelisetti Uma Mahesh: Livestock Research Station, Lam
Farm, Sri Venkateswara Veterinary University, Guntur, Andhra
Pradesh - 522034, India; Department of Biotechnology, University
College, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur,
Andhra Pradesh-522510, India.
Murakonda Mutha Rao: Livestock Research Station, Lam Farm,
Sri Venkateswara Veterinary University, Guntur, Andhra Pradesh -
522034, India
Poda Sudhakar: Department of Biotechnology, University
College, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur,
Andhra Pradesh-522510, India.
Krothapalli Raja Surya Sambasiva Rao: Department of
Biotechnology, University College, Acharya Nagarjuna University,
Nagarjuna Nagar, Guntur, Andhra Pradesh-522510, India.
Received: 28-02-2014, Revised: 03-04-2014, Accepted: 09-04-2014,
Published online: 15-05-2014
Corresponding author: Yelisetti Uma Mahesh, email:
maheshyelisetti@gmail.com, Cell: +91-9849252352
Abstract
Aim: The present study was
aimed at assessing the influence of three oocyte harvesting
(aspiration, puncture and slicing) methods and presence or absence
of corpus luteum (CL) on recovery efficiency and subsequent in
vitro maturation and parthenogenetic development of immature
oocytes recovered from buffalo ovaries.
Materials and Methods: Buffalo ovaries were collected from
local slaughter house and oocytes were collected by using
aspiration, puncture and slicing methods. Collected oocytes were
matured in vitro in TCM-199 medium supplemented with 10% fetal
bovine serum (FBS), 0.22 mM sodium pyruvate, 10 µg/mL of
follicle-stimulating hormone, 6 IU/mL luteinizing hormone, 1 µg/mL
17-β estradiol, 100 IU/mL penicillin and 0.1 mg/mL streptomycin.
In vitro matured oocytes were activated by ethanol (7%) followed
by 6-DMAP (2mM) and cultured to assess the in vitro developmental
capacity.
Results: The average total number of oocytes recovered per
ovary was significantly (P<0.05) higher in slicing (7.88±0.54)
than the aspiration (2.50±0.11) and puncture (3.59±0.18) methods.
The number of culture grade oocytes per ovary was significantly
higher in slicing (5.40±0.29) than in puncture (2.45±0.12) and
aspiration (1.94±0.11) methods. However, the percentage of culture
grade oocytes was higher in the aspiration method (77.67%),
compared to the puncture (68.24%) or slicing (68.51%) methods. The
oocyte recovery was significantly lower (P<0.05) in CL containing
ovaries than that of ovaries without CL (3.01vs.5.17). However,
the presence of CL did not affect the oocytes ability to reach the
MII stage (73.07%vs.75.77%). The results showed that the rates of
Cumulus oocyte complexes (COCs) that reached the metaphase-II
(M-II) stage were similar in aspiration, puncture and slicing
techniques (77.29, 76.12 and 73.92% respectively). The oocytes
obtained were matured and parthenogenetically activated in vitro
using ethanol and 6-DMAP. The oocyte collection methods and
presence or absence of CL did not influence the subsequent
embryonic developmental competence.
Conclusion: It can be concluded that slicing method can be
used as an alternative to aspiration and puncture methods to
recover oocytes from buffalo ovaries; as slicing method resulted
higher oocyte yield and similar in vitro development rate when
compared with other methods.
Keywords: aspiration, buffalo, IVM, oocytes,
parthenogenetic activation, puncture, slicing.
References
1. Delgado, C., Rosegrant, M., Steinfield, H., Ehui, S. and
Courbois C. (1999) Livestock to 2020: The next food
revolution. Food, Agriculture and Environment Discussion.
International Food policy research institute, Washington D.C.,
USA. Food and agriculture organization, Rome, Italy and
International Livestock Research Institute, Addis Ababa,
Ethiopia. p28.
PMCid:PMC325596 |
|
|
|
2. Ingawale, M.V., Bakshi, S.A., Birade, H.S., Chinchkar, S.R.
and Gulavane, S.U. (2012) Studies on follicular development in
Murrah buffaloes with retention of the placenta. Buffalo
Bulletin, 31: 193-201. |
|
|
|
3. Jain, A., Jai, T., Yathish, H.M., Sachjdeva, G.K., Datta,
T.K., De, S., Goswami, S.L. and Patel, R.K. (2011) Headway in
reproductive biotechniques for genetic improvement of
buffaloes. Curr. Trends. Biotechnol. Pharm., 5: 1206-1232. |
|
|
4. Nandi, S., Raghu, H.M., Ravindranatha, B.M. and Chauhan,
M.S. (2002) Production of buffalo (Bubalus bubalis) embryos in
vitro: premises and promises. Reprod. Domest. Anim., 37:
65-74.
http://dx.doi.org/10.1046/j.1439-0531.2002.00340.x
PMid:11975742 |
|
|
5. Drost, M. (2007) Advanced reproductive technology in the
water buffalo. Theriogenology, 68: 450-453.
http://dx.doi.org/10.1016/j.theriogenology.2007.04.013
PMid:17481722 |
|
|
|
6. Agrawal, S.K. and Tommer, O.S. (1998) Reproductive
technologies in Buffalo. Indian Veterinary Research Institute,
Izatnagar, Bareilly, UP, India. p37. |
|
|
7. Krisher, R.L. (2004) The effect of oocyte quality on
development. J. Anim. Sci., 82: E14-23.
PMid:15471793 |
|
|
|
8. Mahesh, Y.U., Rao, M.M. and Rao, K.R.S.S. (2013) Effect of
harvesting technique on recovery rate and in vitro development
of caprine follicular oocytes for in vitro procedures. Indian
J. Anim. Sci., 83: 56-61. |
|
|
9. Zuelke, K.A. and Brackett, B.G. (1990) Luteinizing hormone
enhanced in vitro maturation of bovine oocytes with and
without protein supplementation. Biol. Reprod., 43: 784-787.
http://dx.doi.org/10.1095/biolreprod43.5.784
PMid:2291914 |
|
|
10. Das, G.K., Jain, G.C., Solanki, V.S. and Tripathi, V.N.
(1996) Efficacy of various collection methods for oocyte
retrieval in buffalo. Theriogenology, 46: 1403-1411.
http://dx.doi.org/10.1016/S0093-691X(96)00319-6 |
|
|
|
11. Wang, Z.G., Xu, Z.R. and Yu, S.D. (2007a) Effects of
oocyte collection techniques and maturation media on in vitro
maturation and subsequent embryo development in Boer goat.
Czech. J. Anim. Sci., 52: 21-25. |
|
|
|
12. Wang, Z.G., Yu, S.D. and Xu, S.R. (2007b) Effects of
collection methods on recovery efficiency, maturation rate and
subsequent embryonic developmental competence of oocytes in
Holstein cow. Asian. Australas. J. Anim. Sci., 20: 496-500. |
|
|
|
13. Jamil, H., Samad, A., Qureshi, Z.I., Rehman, N.U. and
Lodhi, L.A. (2008) Harvesting and evaluation of riverine
buffalo follicular oocytes. Turk. J. Vet. Anim. Sci., 32:
25-30. |
|
|
|
14. Mehmood, A., Anwar, M., Andrabi, S.M.H., Afzal, M. and
Naqvi, S.M.S. (2011) In vitro maturation and fertilization of
buffalo oocytes: the effect of recovery and maturation
methods. Turk. J. Vet. Anim. Sci., 35: 381-386. |
|
|
15. Izquierdo, D., Villamediana, P., Lopez Bejar, M. and
Paramio, M.T. (2002) Effect of in vitro and in vivo culture on
embryo development from prepubertal goat IVM- IVF oocytes.
Theriogenology, 57: 1431-1441.
http://dx.doi.org/10.1016/S0093-691X(02)00647-7 |
|
|
16. Otoi, T., Willingham, L., Shin, T., Kraemer, D.C. and
Westhusin, M. (2002) Effects of oocyte culture density on
meiotic competence of canine oocytes. Reproduction, 124:
775-781.
http://dx.doi.org/10.1530/rep.0.1240775
PMid:12530915 |
|
|
|
17. Rao, M.M. and Mahesh, Y.U. (2012) Efficacy of different
harvesting techniques on oocyte retrieval from buffalo
ovaries. Buffalo Bulletin, 31: 209-213. |
|
|
|
18. Hoque, S.A.M., Kabiraj, S.K., Khandoker, M.A.M.Y., Mondal,
A. and Tareq, K.M.A. (2011) Effect of collection techniques on
cumulus oocyte complexes (COCs) recovery, in vitro maturation
and fertilization of goat oocytes. Afr. J. Biotechnol., 10:
9177-9181. |
|
|
19. Wani, N.A., Wani, G.M., Khan, M.Z. and Sidiqi, M.A. (1999)
Effect of different factors on the recovery rate of oocytes
for in vitro maturation and in vitro fertilization procedures
in sheep. Small. Rumin. Res., 34: 71-76.
http://dx.doi.org/10.1016/S0921-4488(99)00046-2 |
|
|
20. Totey, S.M., Singh, G., Taneja, M., Pawshe, C.H. and
Talwer, G.P. (1992) In vitro maturation, fertilization and
development of follicular oocytes from buffalo (Bubalus
bubalis). J. Reprod. Fertil., 95: 597-607.
http://dx.doi.org/10.1530/jrf.0.0950597
PMid:1518014 |
|
|
|
21. Gupta, P.S.P., Nandi, S., Raghu, H.M., Ravindranatha, B.M.
and Sarma, P.V. (2003) Oocyte recovery rates in relation to
morphology and weight of the ovaries in buffaloes. Indian. J.
Anim. Res., 37: 147-148. |
|
|
|
22. Prabhakar, L., Raju, K.G.S., Rao, K. and Rao, K.S. (2012)
Retrieval of oocytes from ovaries of buffalo cows slaughtered
in abattoir. Theriogenology insight, 2: 187-195. |
|
|
|
23. Danell, B. (1987) Oestrous behavior, ovarian morphology
and cyclical variation in follicular system and endocrine
pattern in water buffalo heifers. Ph.D. Thesis, Swedish
University of Agricultural Sciences, Uppsala, Sweden. p124. |
|
|
|
24. Samad, H., Khan, I., Rehman, N. and Ahmad N. (1998) The
recovery, in vitro maturation and fertilization of Nili- Ravi
buffalo follicular oocytes. Asian. Aust. J. Anim. Sci., 11:
491- 497. |
|
|
|
25. Hussain, R., Nematullah. and Akhter, S. (2005) Effect of
season on average number of culturable oocytes recovered from
cattle and buffalo ovaries. Pakistan. J. Zool., 37: 281-283. |
|
|
|
26. El-Gaafary, M.N. and Abdel-Ghaffar, A.E. (1994) In vitro
oocytes maturation, fertilization and cleavage in Egyptian
buffaloes. Annals. Agric. Sci. Moshtohor. Egypt., 32:
1801-1810. |
|
|
27. Zeinoaldini, S., Jafari, Z., Sarmast, F., Torbati, E. and
Davachi, N.D. (2013) Different harvesting techniques used in
ovine in vitro embryo production. Scimetr, 1: e11638.
http://dx.doi.org/10.5812/scimetr.11638 |
|
|
|
28. Singh, S., Dhanda, O.P. and Malik, R.K. Effect of the
presence of corpus luteum on oocyte recovery and subsequent in
vitro maturation and fertilization in buffaloes. Asian.
Australas. J. Anim. Sci., 14: 1675-1677. |
|
|
29. Sahoo, L. and Singla, S.K. (2013) Effect of corpus luteum:
quality and recovery of buffalo (Bubalus bubalis) oocytes.
Turk. J. Vet. Anim. Sci., 37: 468-471.
http://dx.doi.org/10.3906/vet-1204-11 |
|
|
|
30. Hafez, E.S.E. (1993) Folliculogenesis, egg maturation and
ovulation. In: Reproduction in Farm Animals, vol. 1. Lea and
Febiger, Philadelphia. p114-143. |
|
|
31. Varisanga, M.D., Sumantri, C., Murakami, M., Fahrudin, M.
and Suzuki T. (1998) Morphological classification of the
ovaries in relation to the subsequent oocyte quality for IVF
produced bovine embryos. Theriogenology, 50: 1015-1023.
http://dx.doi.org/10.1016/S0093-691X(98)00204-0 |
|
|
32. Abdoon, A.S.S. and Kandil, O.M. (2001) Factors affecting
number of surface ovarian follicles and oocytes yield and
quality in Egyptian buffaloes. Reprod. Nutri. Dev., 41: 71-77.
http://dx.doi.org/10.1051/rnd:2001113
PMid:11368246 |
|
|
|
33. El-Naby, A.A.H.H., Mahmoud, H.K.G.M., Ahmed, Y.F.,
Abouel-Roos, M.E.A. and Abdel- Ghaffar, A.E. (2013) Effect of
season of the year and ovarian structures on oocytes recovery
rate, quality and meiotic competence in Egyptian buffaloes.
Global Veterinaria, 10: 408-412. |
|
|
34. Rao, B.S., Mahesh, Y.U., Lakshmikantan, U.R., Suman, K.,
Charan, K.V. and Shivaji, S. (2010) Developmental competence
of oocytes recovered from postmortem ovaries of the endangered
Indian Blackbuck (Antilope cervicapra). J. Reprod. Dev., 56:
623-629.
http://dx.doi.org/10.1262/jrd.10-059H |
|
|
35. Vajta, G., Machaty, Z., Barrandi, Z.S. and Varga, Z.S.
(1992) Embryos derived from the in vitro fertilization of
pregnant cows. Theriogenology, 37: 811-815.
http://dx.doi.org/10.1016/0093-691X(92)90043-Q |
|
|
|
36. Tasripoo, K. and Kamonpatana, M. (1997) Assessment of
factors contributing to in vitro maturation, fertilization and
cleavage rate of swamp buffalo oocytes. Buffalo J, 3: 325-
336. |
|
