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Research
(Published
online: 16-11-2014)
12.
The use of Na+ and K+ ion concentrations as
potential diagnostic indicators of subclinical mastitis in dairy
cows -
Abdul Wahid Haron, Faez Firdaus Jesse
Abdullah, Abdulnasir Tijjani, Yusuf Abba, Lawan Adamu, Konto
Mohammed, Aisyah Munira Mohammed Amir, Mohammad Abubakar Sadiq and
Mohd Azmi Mohd Lila
Veterinary World, 7(11): 966-969
doi:
10.14202/vetworld.2014.966-969
Abdul Wahid Haron:
Department of Veterinary Clinical Studies, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Research Centre
for Ruminant Disease, Faculty of Veterinary Medicine, Universiti
Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; wahidharon@gmail.com
Faez Firdaus Jesse Abdullah:
Department of Veterinary Clinical Studies, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Research
Centre for Ruminant Disease, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;
jesseariasamy@gmail.co
Abdulnasir Tijjani:
Department of
Veterinary Pathology and Microbiology, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia; nasirvet69@gmail.com
Yusuf Abba:
Department of Veterinary Pathology and Microbiology, Universiti
Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; yabbavet@gmail.com
Lawan Adamu:
Department of Veterinary Clinical Studies, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia; drlawan3758@yahoo.com
Konto Mohammed:
Department of Veterinary Clinical Studies, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia; kontomohammed@yahoo.com
Aisyah
Munira Mohammed Amir:
Department of Veterinary Clinical Studies, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia; aisyahmunira@gmail.com
Mohammad Abubakar Sadiq:
Department of Veterinary Clinical Studies, Universiti Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia; masadiqvet@gmail.com
Mohd
Azmi Mohd Lila: Department of Veterinary Pathology and
Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang,
Selangor,
Malaysia;
azmi@upm.edu.my
Received: 08-07-2014, Revised: 12-10-2014, Accepted: 21-10-2014,
Published online: 16-11-2014
Corresponding author:
Abdul Wahid Haron, e-mail: wahidharon@gmail.com
Abstract
Aim:
This study was conducted to evaluate the concentrations of
sodium (Na+) and potassium (K+) ions in milk of lactating dairy
cows with and without subclinical mastitis as putative indicators
for detecting subclinical mastitis in dairy cows.
Materials and Methods: Thirty seven lactating dairy cows were
screened for the evidence of subclinical mastitis using California
mastitis test (CMT). The lactating dairy cows were categorized as
CMT-Positive (CMT-P; n=20) and CMT-Negative (CMT-N; n=17) based on
whether they were positive or negative for CMT using a standard
kit. The CMT-P lactating dairy cows were further sub divided into
subclinical 1+ (S1+; n=6), subclinical 2+ (S2+; n=9), and
subclinical 3+(S3+; n=5). Direct microscopy somatic cell count (SCC)
was used to determine the SCC using Wright’s stain. The samples
were filtered and diluted at 1:100 dilutions before being measured
for the concentrations of Na+ and K+ using atomic absorption
spectrophotometer.
Results: There was a significant increase (p<0.05) in SCCs and
Na+ concentration in the milk of CMT-P dairy cows, with a mean
Log10 SCC score of 5.35±0.06 cells/ml and mean Na+ concentration
of 232±19.1 mg/dL. However, there was a significant reduction
(p<0.05) in the concentration of K+ (123±7.6 mg/dL) in the milk
samples of the CMT-P cows. There were significant differences
(p<0.05) in SCC, Na+ and K+ concentrations between milk samples
from the CMT-N dairy cows and CMT-P subgroups; S1+, S2+, and S3+
respectively. Potassium (K+) concentration had a significant
strong negative correlation with sodium (Na+) concentration
(r=−0.688; p<0.01) and weak positive correlation with SCC
(r=−0.436; p<0.01). The sensitivity of using Na+ and K+
concentrations as detection indices for sub-clinical mastitis is
40% and 90%, respectively, while the specificity of each was 100%.
Conclusion: This study thus shows that evaluation of Na+ and
K+ concentrations from milk samples of dairy cows with sub
clinical mastitis can be of immense diagnostic benefit and will
compliments detection using CMT alone.
Keywords: potassium ion, sodium ion, somatic cell count,
subclinical mastitis.
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