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Research
(Published
online: 19-11-2014)
14.
Some biochemical
constituents in follicular fluid of indigenous cows of Assam
- S. S. Deka, D. J. Kalita, S. Sarma and D. J. Dutta
Veterinary World, 7(11): 976-979
doi:
10.14202/vetworld.2014.976-979
S. S.
Deka:
Department of Veterinary Biochemistry, College of Veterinary
Science, Assam Agricultural University, Guwahati, Khanapara,
Assam, India;
surakshasubedi@gmail.com
D. J.
Kalita:
Department of Veterinary Biochemistry, College of Veterinary
Science, Assam Agricultural University, Guwahati, Khanapara,
Assam, India;
djkalita@rediiffmail.com
S.
Sarma:
Department of Veterinary Biochemistry, College of Veterinary
Science, Assam Agricultural University, Guwahati, Khanapara,
Assam, India;
sarmasatya49@yahoo.in
D. J.
Dutta: Department of Veterinary Biochemistry, College of
Veterinary Science, Assam Agricultural University, Guwahati,
Khanapara, Assam, India;
duttadj@hotmail.com
Received:
28-07-2014, Revised: 02-10-2014, Accepted: 09-10-2014, Published
online: 19-11-2014
Corresponding author:
D. J. Kalita, e-mail: djkalita@rediiffmail.com
Abstract
Aim:
Estimation of some biochemical constituents in follicular
fluid of non-descript cows of Assam.
Materials and Methods: Twenty five pairs of ovaries were
collected from local slaughter house and the follicular fluid was
aspirated from small (2-4 mm), medium (4-6 mm) and large (6-9 mm)
follicles. Aspirated fluid samples were centrifuged at 4000 rpm
for 20 min in a refrigerated centrifuge to remove granulosa cells
and other cell debris. Supernatant was used for estimation of
glucose, total protein, cholesterol, acid phosphatase and alkaline
phosphatase. Data generated in the study were analyzed statiscally
by SPSS (version 16.0). SPSS South Asia Pvt. Limited,
Kacharakanahalli, Bangalore, 560043).
Results: A non significant difference was recorded in case of
total protein and cholesterol of follicular fluid of small, medium
and large sized follicles of cow. However, the glucose level
significantly (p<0.01) increased with the increase of follicular
size where as the activity of acid phosphatase and alkaline
phosphatase significantly (p<0.01) decreased with increase of
size.
Conclusion: Certain biochemical constituents and enzyme
activities of follicular fluid changes with the growth of
follicles in non-descript cows of Assam. The glucose concentration
increased with the growth of the follicles while acid phosphatase
and alkaline phosphatase levels had an inverse relation with the
size of the ovarian follicles.
Keywords: biochemical parameters, follicles, follicular fluid,
oocytes maturation, phosphatase.
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