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Research (Published online: 27-11-2014)

17. Evaluation of antibacterial effect of some Sinai medicinal plant extracts on bacteria isolated from bovine mastitis - Gamil S. G. Zeedan, Abeer M. Abdalhamed, Eman Abdeen, Mahmoud E. Ottai and Sobhy Abdel-Shafy

Veterinary World, 7(11): 991-998



   doi: 10.14202/vetworld.2014.991-998



Gamil S. G. Zeedan: Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Center, Dokki,

Giza, Egypt;

Abeer M. Abdalhamed: Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Center, Dokki,

Giza, Egypt;

Eman Abdeen: Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, University of Sadat City, Monifia, Egypt;

Mahmoud E. Ottai: Department of Genetics and Cytology, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Giza, Egypt;

Sobhy Abdel-Shafy: Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt;


Received: 21-07-2014, Revised: 15-10-2014, Accepted: 22-10-2014, Published online: 27-11-2014


Corresponding author: Gamil S. G. Zeedan, e-mail:

Aim: Bovine mastitis is the most economically important disease affecting dairy cattle worldwide from an economic, diagnostic and public-health point of view. The present study aimed to isolate and identify of bacteria causes mastitis in dairy cows and to evaluate the antibacterial activities of some selected medicinal plants extracts comparing antibiotics used in the treatment of mastitis in Egypt.

Materials and Methods: A total of 203 milk samples of dairy cows were collected during the period from February to June 2013 at different Governorates in Egypt. The use clinical inspection and California mastitis test examination were provided efficient diagnostic tool for detection of clinical, subclinical mastitis and apparently normal health cattle. The collected milk samples were cultured on Nutrient, Blood agar, Mannitol salt, Edward’s and MacConkey agar plates supporting the growth of various types of bacteria for their biochemical studies and isolation. The antimicrobial activity of plants extracts (Jasonia montana and Artemisia herb alba)with different solvent (ethanol, petroleum ether, chloroform and acetone)were studied in vitro against isolated bacteria from mastitis by paper desk diffusion and minimum inhibitory concentration method (MIC).

Results: The prevalence of clinical, subclinical mastitis and normal healthy animals were 34.50%, 24.7% and 40.8% respectively. The major pathogens isolated from collected milk samples were Escherichia coli (22.16%), Staphylococcus aureus (20.19%), Streptococcus spp. (13.3%), Streptococcus agalactiae (12.8%), Streptococcus dysgalactia (0.5%), Pasteurella spp. (2.45%), Klebsiella spp. (1.47%)and Pseudomonas spp. (0.45%). The highest antibacterial activity of J. montana plant extracted with acetone solvent against S. agalactiae, E. coli, S. aureus, Klebsiella spp and coagulase-negative Staphylococci with zone of inhibition values ± standard deviation (SD), ranging from 4.33±0.57 to 25.6±0.60 mm. The MIC values for the extracts ranged from 0.01 to 1.56 mg/ml. when comparing antibacterial activity of A. herb alba plant extracted with acetone solvent on the same bacteria with zone of inhibition values ± SD, ranging from 00±00 to 5.6±0.60 mm. Both extracts from J. montana and A. herb alba plant extracts with petroleum ether, methanol and chloroform solvent were less antibacterial activities than acetone solvent extract.

Conclusion: The present study spot highlight on isolation and identification of mastitis pathogens that are fundamental aspects of milk quality, udder health control programs and public health and food safety issues associated with food borne pathogens. J. montana and A. herb alba plants have antibacterial effects more than antibiotics used in the treatment of mastitis. Finally, the medicinal plant extracts can be used to discover bioactive natural product in the form of antibacterial that may be serve the development of new pharmaceutical products. But still need further research necessary to identify active compounds and research to mechanism and drug interaction.

Keywords: antimicrobial agent, Artemisia herb alba, California mastitis test, Jasonia montana, minimum inhibitory concentration.

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