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Research
(Published
online: 27-11-2014)
18.
Seroepidemiological
pattern of leptospirosis in bovine of South Gujarat, India
- J. M. Patel, P. D.Vihol, M. C. Prasad, I. H. Kalyani, J. K.
Raval, K. M. Patel, Sushma R. A. Thirumalesh and V. Balamurugan
Veterinary World, 7(11): 999-1003
doi:
10.14202/vetworld.2014.999-1003
J. M.
Patel: Department
of Veterinary Pathology, Vanbandhu College of Veterinary Sciences
& Animal Husbandry, Navasari Agricultural University, Navsari,
Gujarat, India;
dr.jams@rediffmail.com
P. D.
Vihol: Department
of Veterinary Pathology, Vanbandhu College of Veterinary Sciences
& Animal Husbandry, Navasari Agricultural University, Navsari,
Gujarat, India;
drpriti@nau.in
M. C.
Prasad: Department
of Veterinary Pathology, Vanbandhu College of Veterinary Sciences
& Animal Husbandry, Navasari Agricultural University, Navsari,
Gujarat, India;
drmcprasad1938@gmail.com
I. H.
Kalyani:
Department of Veterinary Microbiology, Vanbandhu College of
Veterinary Sciences & Animal Husbandry, Navasari Agricultural
University, Navsari, Gujarat, India;
ihkvet@gmail.com
J. K.
Raval: Department
of Veterinary Pathology, Vanbandhu College of Veterinary Sciences
& Animal Husbandry, Navasari Agricultural University, Navsari,
Gujarat, India;
ravaljk2@gmail.com
K. M.
Patel: Veterinary
Dispensary, Patan District Panchayat, Department of Animal
Husbandry, Varahi, Patan, Gujarat, India;
kmplpt@yahoo.com
Sushma
R. A. Thirumalesh:
Project Directorate on
Animal Disease and Survellance, Indian Council of Agricultural
Research, Hebbal, Bengaluru, Karnataka, India;
srath@gmail.com
and V.
Balamurugan:
Project Directorate on Animal Disease and Survellance, Indian
Council of Agricultural Research, Hebbal, Bengaluru,
Karnataka, India;
balavirol@gmail.com
Received:
02-08-2014, Revised: 25-10-2014, Accepted: 03-11-2014, Published
online: 27-11-2014
Corresponding author: J. M. Patel,
e-mail: dr.jams@rediffmail.com
Abstract
Aim:
Seroepidemiological study of leptospirosis in cattle of
various South Gujarat district (Navsari, Tapi, Surat, Valsad).
Materials and Methods: Whole blood samples were collected
randomly from different age groups, and breeds of cattle of either
sex reared in different districts (Navsari, Surat, Tapi, Valsad)
of South Gujarat. To obtain serum, whole blood was kept in
slanting position in 9.0 ml plain vacutainers until serum
extracted out of the whole blood. Then these 9.0 ml plain
vacutainers were centrifuged at 7000 rpm for 10 min. The straw
colored serum was then collected in 1.5 ml sterile cryo vials and
aliquoted and stored at −20°C for
microscopic agglutination test.
Results: In the present study, overall 12.81% (51/398)
seroprevalence were recorded with highest seroprevalence (47.06%,
24/51) from Valsad followed by Navsari (9.14%, 18/197), Surat
(6.90%, 2/29) and Tapi (5.79%, 7/121) among cattle. The
seroprevalence rate of breed and sex wise did not differ
significantly (p≤0.05). Maximum
incidence of seropositivity was found above 4 years (16.32%,
39/239) of age group followed by animals between 1 and 4 years
(9.68%, 12/124). Thus, the age was significantly influencing the
seropositivity (p≤0.05). In cattle
out of 398 sera screened, 51 were positive with one or more
serovars. The highest seropositivity was recorded against serovar
Pomona (28.89%).
Conclusions: Overall 12.81% seroprevalence of leptospirosis in
apparently healthy and clinically ailing bovine of South Gujarat
indicating potential zoonotic risk to farmers, labor, and animal
owners.
Keywords: cattle, leptospirosis, seroepidemiology, zoonosis.
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