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Research
(Published
online: 30-11-2014)
21.
The effectiveness of novel bacteriocin
derived from Escherichia coli colonized in the fermented
pineapple Ananas comosus (L.) Merr. against pathogenic
bacteria isolated from aquaculture sites -
S. W. Lee, W. Wendy,
L. Montira and A. U. Siti Hazirah
Veterinary World, 7(11): 1014-1018
doi:
10.14202/vetworld.2014.1014-1018
S. W.
Lee:
Faculty of Agro Based Industry, Universiti Malaysia Kelantan
Campus Jeli, 17600, Jeli, Kelantan, Malaysia;
leeseongwei@yahoo.com
W.
Wendy:
School of Fisheries and Aquaculture Sciences, Universiti Malaysia
Terengganu, Kuala Terengganu, 21030, Terengganu,
Malaysia;
wendy@umt.edu.my
L.
Montira:
Department of Science, Biology Division, Faculty of Science and
Technology, Prince of Songkla University, Pattani Campus, Pattani,
94000, Thailand;
montiral@yahoo.com
A. U.
Siti Hazirah:
Faculty of Agro Based Industry, Universiti Malaysia Kelantan
Campus Jeli, 17600, Jeli, Kelantan, Malaysia;
hazirah@yahoo.com
Received:
07-07-2014, Revised: 21-09-2014, Accepted: 24-09-2014, Published
online: 30-11-2014
Corresponding author:
S. W. Lee, e-mail: leeseongwei@yahoo.com
Abstract
Aim:
The aim was to evaluate antimicrobial property of bacteriocin
isolated from Escherichia coli against pathogenic bacteria
from aquaculture sites.
Materials and Methods: E. coli was isolated from
fermented pineapple Ananas comosus using eosin methylene
blue agar. The antimicrobial activity of the isolated E. coli
was screened using hole-plate diffusion method. The bacterial
strain that showed the widest inhibition zone was selected and
grown in tryptic soy broth, followed by partial purification of
bacteriocin by using ammonium sulphate. Bacteriocin derived from
the E. coli was subjected to the antimicrobial test against
55 bacteria strains namely Aeromonas hydrophila (n=10),
Citrobacter freundii (n=5), Edwardsiella tarda (n=10),
Flavobacterium spp. (n=10), Pseudomonas spp. (n=10),
Vibrio parahaemolyticus (n=5) and Vibrio alginolyticus
(n=5) by using twofold broth microdilution method to determine
minimum inhibitory concentration (MIC) values of the bacteriocin
against the tested bacteria.
Results: The results of the present study showed that the MIC
values of the partially purified bacteriocin against present
pathogenic bacteria isolates ranged from 7.81 to 31.25 ppm whereas
the MIC values of kanamycin (positive control) ranged from 15.63
to 125 ppm.
Conclusion: The results of the present study showed the
bacteriocin derived from E. coli can control all the
present bacterial isolates indicating the huge potential of the
bacteriocin as a new antimicrobial agent for aquaculture uses.
Keywords: Escherichia coli, partially
purified bacteriocin, pathogenic bacteria.
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