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Research
(Published
online: 30-11-2014)
2 2.
Bone marrow derived cell-seeded
extracellular matrix: A novel biomaterial in the field of wound
management - V. Remya, Naveen Kumar, A. K. Sharma,
Dayamon D. Mathew, Mamta Negi, S. K. Maiti, Sameer Shrivastava, Sonal and N. P. Kurade
Veterinary World, 7(11): 1019-1025
doi:
10.14202/vetworld.2014.1019-1025
V.
Remya:
Division of Surgery, Indian Veterinary Research Institute,
Izatnagar, Uttar Pradesh, India;
remyamukund04vet@gmail.com
Naveen
Kumar:
Division of Surgery, Indian Veterinary Research Institute,
Izatnagar, Uttar Pradesh, India;
naveen.ivri1961@gmail.com
A. K.
Sharma:
Division of Surgery, Indian Veterinary Research Institute,
Izatnagar, Uttar Pradesh, India;
ashok.sharma1010@gmail.com
Dayamon
D. Mathew:
Division of Surgery, Indian Veterinary Research Institute,
Izatnagar, Uttar Pradesh, India;
dayamon@gmail.com
Mamta
Negi:
Division of Surgery, Indian Veterinary Research Institute,
Izatnagar, Uttar Pradesh, India;
mamtaanegi@gmailcom
S. K.
Maiti:
Division of Surgery, Indian Veterinary Research Institute,
Izatnagar, Uttar Pradesh, India;
maiti_62@rediffmail.com
Sameer
Shrivastava:
Division of Veterinary Biotechnology, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India;
sameer_vet@rediffmail.com
Sonal:
Division of Veterinary Biotechnology, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India;
sonalvet@gmail.com
N. P.
Kurade: Division of Pathology, Indian Veterinary Research
Institute, Izatnagar, Uttar Pradesh, India;
npkurade@yahoo.co.in
Received:
04-06-2014, Revised: 19-10-2014, Accepted: 24-10-2014, Published
online: 30-11-2014
Corresponding author:
V. Remya: e-mail: remyamukund04vet@gmail.com
Abstract
Aim:
Extensive or irreversible damage to the skin often requires
additional skin substitutes for reconstruction. Biomaterials have
become critical components in the development of effective new
medical therapies for wound care.
Materials and Methods: In the present study, a cell matrix
construct (bone marrow-derived cells (BMdc) seeded extracellular
matrix [ECM]) was used as a biological substitute for the repair
of full-thickness skin wound. ECM was developed by decellularizing
fish swim bladder (FSB). Goat bone marrow-derived cells (G-BMdc)
were seeded over this decellularized matrix. Efficacy of this cell
matrix construct in wound repair was tested by implanting it over
20 mm2 × 20 mm2 size fullthickness skin wound
created over the dorsum of rat. The study was conducted in 16
clinically healthy adult rats of either sex. The animals were
randomly divided into 2 equal groups of 8 animals each. In Group
I, animal’s wounds were repaired with a cellular FSB matrix. In
Group II, wounds were repaired with G-BMdc seeded a cellular FSB
matrix. Immune response and efficacy of healing were analyzed.
Results: Quality of healing and immuno tolerance to the
biological substitute was significantly better in Group II than
Group I.
Conclusion: Seeding with BMdc increases the wound healing
potency and modulates the immune response to a significantly
negligible level. The BMdc seeded acellular FSB matrix was found
to be a novel biomaterial for wound management.
Keywords: biomaterial, decellular, extra cellular matrix,
wound.
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