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Research
(Published
online: 08-11-2014)
5.
Polymerase chain reaction based
epidemiological investigation of canine parvoviral disease in dogs
at Bareilly region - Jobin Thomas, Mithilesh Singh, T.
K. Goswami, Suman Verma and Surendra Kumar Badasara
Veterinary World, 7(11): 929-932
doi:
10.14202/vetworld.2014.929-932
Jobin
Thomas:
Department of Immunology, Indian Veterinary Research Institute,
Bareilly, Uttar Pradesh, India;
jobss2k6@gmail.com
Mithilesh Singh:
Department of Immunology, Indian Veterinary Research Institute,
Bareilly, Uttar Pradesh, India; rmithileshsingh@yahoo.com
T. K.
Goswami:
Department of Immunology, Indian Veterinary Research Institute,
Bareilly, Uttar Pradesh, India;
goswami.tapas@gmail.com
Suman
Verma:
Department of Immunology, Indian Veterinary Research Institute,
Bareilly, Uttar Pradesh, India;
suman0751@gmail.com
Surendra Kumar Badasara:
Department of Immunology, Indian Veterinary Research Institute,
Bareilly, Uttar Pradesh, India;
badsara.vet@gmail.com
Received:
18-07-2014, Revised: 19-09-2014, Accepted: 24-09-2014, Published
online: 08-11-2014
Corresponding author:
Mithilesh Singh, e-mail: drmithileshsingh@yahoo.com
Abstract
Aim:
The aim of this study was to screen the suspected samples by
polymerase chain reaction (PCR) and epidemiological analysis of
positive cases of canine parvovirus type2.
Materials and Methods: Fecal samples were collected from dogs
suspected for canine parvovirus type 2 (CPV-2) and viral DNA was
extracted. Primers were designed, and PCR was done with all
extracted DNA samples. Age, sex and breed wise distribution of
positive cases were analyzed.
Results: Out of a total 44 collected fecal samples, 23 were
found to be positive for CPV-2 by developed PCR. The disease was
found to be more common in Labrador male pups of 3-6 months of
age. The percentage of positive cases in vaccinated dogs was found
to be around 17.4%.
Conclusion: Almost half (52.3%) of total collected samples
were found to be positive by PCR. However, number of field samples
are needed to further validate this test and additionally sequence
analysis needs to be done to ensure the prevalent field strain of
CPV-2.
Keywords: Canine parvovirus type 2, fecal sample, polymerase
chain reaction.
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