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Research (Published online: 08-11-2014)

5. Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region - Jobin Thomas, Mithilesh Singh, T. K. Goswami, Suman Verma and Surendra Kumar Badasara

Veterinary World, 7(11): 929-932



   doi: 10.14202/vetworld.2014.929-932



Jobin Thomas: Department of Immunology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;

Mithilesh Singh: Department of Immunology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;

T. K. Goswami: Department of Immunology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;

Suman Verma: Department of Immunology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;

Surendra Kumar Badasara: Department of Immunology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;


Received: 18-07-2014, Revised: 19-09-2014, Accepted: 24-09-2014, Published online: 08-11-2014


Corresponding author: Mithilesh Singh, e-mail:

Aim: The aim of this study was to screen the suspected samples by polymerase chain reaction (PCR) and epidemiological analysis of positive cases of canine parvovirus type2.

Materials and Methods: Fecal samples were collected from dogs suspected for canine parvovirus type 2 (CPV-2) and viral DNA was extracted. Primers were designed, and PCR was done with all extracted DNA samples. Age, sex and breed wise distribution of positive cases were analyzed.

Results: Out of a total 44 collected fecal samples, 23 were found to be positive for CPV-2 by developed PCR. The disease was found to be more common in Labrador male pups of 3-6 months of age. The percentage of positive cases in vaccinated dogs was found to be around 17.4%.

Conclusion: Almost half (52.3%) of total collected samples were found to be positive by PCR. However, number of field samples are needed to further validate this test and additionally sequence analysis needs to be done to ensure the prevalent field strain of CPV-2.

Keywords: Canine parvovirus type 2, fecal sample, polymerase chain reaction.

1. Taguchi, M., Namikawa, K., Maruo, T., Lynch, J. and Sahara, H. (2010) Antibodies to parvovirus, distemper virus and adenovirus conferred to household dogs using commercial combination vaccines containing leptospira bacterin. Vet. Rec., 167: 931-934.
2. Naidua, H., Subramaniana, B.M., Chinchkara, S.R. Sriramana, R., Ranac, S.K. and Srinivasana, V.A. (2012) Typing of canine parvovirus isolates using mini-sequencing based single nucleotide polymorphism analysis. J. Virol. Methods, 181: 197-201.
3. Cavalli, A., Martella, V., Costantina, D., Michele, C., Anna, L.B., Pasquale, D.P., Nicola, D., Gabriella, E. and Buonavoglia, C. (2008) Evaluation of the antigenic relationships among canine parvovirus type 2 variants. Clin. Vaccine Immunol., 15(3): 534-539.
PMid:18160619 PMCid:PMC2268271
4. Nandi, S., Chidri, S., Kumar, M. and Chauhan, R.S. (2010) Occurrence of canine parvovirus type 2c in the dogs with haemorrhagic enteritis in India. Res. Vet. Sci., 88: 169-171.
5. Decaro, N., Desario, C., Campolo, C., Cavalli, A., Ricci, D., Martella, V., Tempesta, M. and Buonavoglia, C. (2004) Evaluation of the lactogenic immunity to canine parvovirus in pups. New, Microbiol, 27: 375-379.
6. Elia, G., Desario, C., Pezzoni, G., Camero, M., Brocchi, E., Decaro, N., Martella, V. and Buonavoglia, C. (2012) Recombinant ELISA using baculovirus-expressed VP2 for detection of antibodies against canine parvovirus. J. Virol. Methods, 184: 98-102.
7. Parthiban, M., Kurunchi, C., Divya, K., Kumanan, K. and Bargavi, D.S. (2012) A rapid and highly reliable field-based LAMP assay of canine parvovirus. Acta. Virol., 56: 71-74.
8. Kumar, M., Chidri, S. and Nandi, S. (2010) Molecular cloning and restriction endonuclease analysis of canine parvovirus DNA amplified by polymerase chain reaction. Glob. Vet., 4(2): 125-129.
9. Houston, D.M., Ribble, C.S. and Head, L.L. (1996) Risk factors associated with parvovirus enteritis in dogs. J. Am. Vet. Med. Assoc., 208: 542-548.
10. Khan, M.A., Rabbni, K., Muhammad, K., Murtaza, N and Nazir, J. (2006) Isolation and characterization of canine parvo virus. Int. J. Agric. Biol., 156: 898-900.
11. Mittal, M., Chakravarti, S., Mohapatra, J.K., Chug, P.K., Dubey, R., Upamanuyu, V., Narwal, P.S., Kumar, A., Churamani, C.P. and Kanwar, N.S. (2014) Molecular typing of canine parvovirus strains circulating from 2008 to 2012 in an organized kennel in India reveals the possibility of vaccination failure. Infect, Genet. Evol., 23: 1-6.
12. Schultz, R. (2006) Duration of immunity for canine and feline vaccines: A review. Vet. Microbiol., 117(1): 75-79.
13. Schultz, R.L. (2008) Current canine parvovirus type 2 (CPV-2) vaccines provide excellent immunity to all genotypes of CPV-2 (e.g. CPV-2a, 2b, and 2c). Vet. Ther., 9(2): 94-101.
14. Decaro, N., Desario, C., Billi, M., Mari, V., Elia, G., Cavalli, A., Martella, V. and Buonavoglia, C. (2011) Western European epidemiological survey for parvovirus and coronavirus infections in dogs. Vet. J., 187: 195-199.
15. Nandi, S., Ambazhagan, R. and Kumar, M. (2010) Molecular characterization and nucleotide sequence analysis of canine parvovirus strains in vaccine in India. Vet. Ital., 46(1): 69-81.