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Research (Published online: 13-11-2014)

8. Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in Chhattisgarh - Govina Dewangan, S. D. Hirpurkar, Devesh Kumar Giri and Deepak Kumar Kashyap

Veterinary World, 7(11): 943-947



   doi: 10.14202/vetworld.2014.943-947



Govina Dewangan: Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Durg, Chhattisgarh, India;

S. D. Hirpurkar: Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Durg, Chhattisgarh, India;

Devesh Kumar Giri: Department of Veterinary Pathology, Uttar Pradesh, Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evan Go Anusandhan Sansthan, Mathura, Uttar Pradesh, India;

Deepak Kumar Kashyap: Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Arawali Veterinary College, Sikar, Rajasthan, India;


Received: 26-07-2014, Revised: 05-10-2014, Accepted: 10-10-2014, Published online: 13-11-2014


Corresponding author: Govina Dewangan, e-mail:

Aim: The objective of the research was to assess the innate immunity status of non-descript (ND) cattle in comparison to Sahiwal cattle in Chattisgarh by the determination of the phagocytosis activity (PA) and opsonocytophagic index (OI).

Materials and Methods: In-vitro phagocytic activity of neutrophil was studied in ND and Sahiwal (S) cattle after dividing them in groups viz. ND-I, ND-II, S-I and S-II. Neutrophils were separated from the blood and activated with Staphylococcus aureus. PA and OI were observed under high power of a microscope at 1000  magnification.

Results: PA (percent) in groups ND-I, ND-II, S-I and S-II was 73.001.03, 82.251.51, 80.751.36 and 82.371.27, respectively which vary significantly (p<0.01), with respect to age. The observations regarding the OI (bacteria per neutrophil) in groups ND-I, ND-II, S-I and S-II was 7.170.04, 7.760.09, 7.020.04 and 7.790.06, respectively. Age difference was evident, i.e., OI increases as age increases.

Conclusion: Innate immunity plays a vital role in host defense against diseases. Neutrophil kills the invading pathogen by phagocytic action. PA and OI is an important tool for the assessing innate immune status. After coincubation of neutrophil with bacterial cells, PA was significantly lower (p<0.01) in ND-I, calves group then other groups. Further, the result of OI clearly indicates that OI has increased as age increases in both breeds. The study will, therefore, generate first hand information on ND cattle along with their relative statistical differences, if any, when compared with Sahiwal.

Keywords: innate immunity, non-descript cattle, opsonocytophagic index, phagocytosis activity.

1. Chhattisgarh Government Livestock Census. (2007) Districtwise details of livestock census. Available from:
2. Tomar, S.S. (2010). Textbook of Animal Breeding. Kalyani Publishers, New Delhi, India.
3. Nqeno, N. (2008) Reproductive performance of cows in sweet and sour field types under communal production systems in Eastern Cape Province of South Africa. Master of Science in Animal Science Thesis Submitted to University of Fort Hare.
4. Nivsarkar, A.E., Vij, P.K. and Tantia, M.S. (2000) Animal Genetic Resources of India Cattle and Buffalo. Vinayak Press, New Delhi, India.
5. Chande, R.S.S. (2011) Characterization of local cows of northern hilly region of Chhattisgarh. M. V. Sc. Thesis Submitted to Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.).
6. Janeway, J. and Medzhitov, R. (2002) Innate immune recognition, Annu. Rev. Immunol., 20: 197-116.
7. Kantari, C., Pederzoli, M. and Witko S.V. (2008) The role of neutrophils and monocytes in innate immunity. Contrib. Microbiol., 15: 118-146.
8. Mohapatra, A., Swain, D.K., Pathan, M.M.K., Kaur, M. Panigrahy, S.R., Kapila, S., Kapila, R., Prasad, S., Mohanty, A.K. and Dang, A.K. (2012) Scanning electron microscopy and phagocytic activity of blood and milk neutrophils isolated from early lactating buffaloes. Indian J. Dairy Sci., 65(6): 479-483.
9. Mehrzad, J., Duchateau, L. and Burvenich, C. (2004) Viability of milk neutrophils and severity of bovine coliform mastitis. J. Dairy Sci., 87: 4150-4162.
10. Samtani, J.R., Wagle, S.U., Irani, S.F. and Kshirsagar, N.A. (1991) Micromethod for PMN function studies in neonates. J. Postgrad. Med., 37: 160.
11. Ordonez, V.V., Salas, N.P., Gorodezky, C. and Oaxaca, J.S. (2008) In-vitro differential neutrophil phagocytosis activity on Staphylococcus aureus when obtained from blood and milk of dairy cows in early lactation period. Rev. Latinoam. Microbiol., 50: 66-71.
12. Malik, B.S. (1986) A Laboratory Manual of Bacteriology, Mycology & Immunology. CBS Publishers and Distributors, Delhi.
13. Snedecor, G.W. and Cochran, W.G. (1994) Statistical Methods. 8th ed. Iowa State University Press Ames, Iowa, USA.
14. Vishnoi, P.C., Dang, A.K. and Kapila, S. (2007) In vitro phagocytic activity of neutrophils isolated from blood, milk of Murrah buffaloes with clinical mastitis. Buffalo J., 1: 51-59.
15. Guidry, A.J., Pappe, M.J. and Miller, R.H. (1974) In vitro procedure for measuring phagocytosis of blood neutrophils. Am. J. Vet. Res., 35: 705-709.
16. Dosogne, H., Vangroenweghe, F., Barrio, B., Rainard, P. and Burvenich, C. (2001) Decreased number and bactericidal activity against staphylococcus aureus of the resident cells in milk of dairy cows during early lactation. J. Dairy Res., 68: 539-549.
17. Windsor, M. (2012) A simple no-wash method for the flow cytometric enumeration of leukocyte subsets in whole blood. Excerpt MACS More., 14: 26-29.
18. Choi, E.Y., Santoso, S. and Chavakis, T. (2009) Mechanisms of neutrophil transendothelial migration, Front Biosci., 14: 1596-1605.
19. Kassinen, S.S. (1986) Optimal conditions for the opsonophagocytosis test with streptococcus pneumonia serotypes 3, 6A, 7F and 19F and human granulocytes. Acta Pathol. Microbiol. Immunol. Scand., 94(3): 105-111.
20. Rainard, P. and Riollet, C. (2003), Mobilization of neutrophils and defense of bovine mammary gland. Reprod. Nutr. Dev., 43: 439-457.