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Research (Published online: 15-10-2014)

13. Prevalence of Rotavirus in shellfish from Southern Kerala - Vysakh Mohan, Shriya Rawat, K. M. Lokesh, H. V. Mohan, D. Avinash Reddy, Ashok Kumar and K. N. Bhilegaonkar

Veterinary World, 7(10): 821-824



   doi: 10.14202/vetworld.2014.821-824



Vysakh Mohan: Division of Veterinary Public Health, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;

Shriya Rawat: Department of Veterinary Public Health, Sardar Vallabhai Patel College of Veterinary and Animal Sciences, Meerut, Uttar Pradesh, India;

K. M. Lokesh: Division of Veterinary Public Health, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India;

H. V. Mohan: Department of Veterinary Public Health, Veterinary College, Bidar, Karnataka, India;

D. Avinash Reddy: Division of Veterinary Public Health, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;

Ashok Kumar: Division of Veterinary Public Health, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;

K. N. Bhilegaonkar: Division of Veterinary Public Health, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India;


Received: 21-06-2014, Revised: 26-08-2014, Accepted: 02-09-2014, Published online: 14-10-2014


Corresponding author: Vysakh Mohan, e-mail:

Aim: To study the prevalence of Rotavirus in shellfish from Southern Kerala.

Materials and Methods: The shellfish samples after processing was concentrated using proteinase K. RNA was isolated from the concentrated samples using phenol chloroform method. Rota viral RNA was detected using reverse transcriptionpolymerase chain reaction.

Results: A low prevalence of 2.5% (5/200) was obtained during the study. Rotavirus was detected in 2 out of 60 brown mussels (3.33%), 2 out of 70 yellow clams (2.86%) and 1 out of 70 black clams (1.43%).

Conclusion: Low prevalence of Rotavirus was obtained in our study. A more extensive study need to be conducted to estimate the prevalence of enteric virus in shellfish.

Keywords: concentration, proteinase K, reverse transcription-polymerase chain reaction, Rotavirus, shellfish.

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