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Research
(Published
online: 15-10-2014)
13.
Prevalence of Rotavirus in shellfish
from Southern Kerala -
Vysakh Mohan, Shriya
Rawat, K. M. Lokesh, H. V. Mohan, D. Avinash Reddy, Ashok Kumar and
K. N. Bhilegaonkar
Veterinary World, 7(10): 821-824
doi:
10.14202/vetworld.2014.821-824
Vysakh
Mohan:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India;
vysakhmohanvet@gmail.com
Shriya
Rawat:
Department of Veterinary Public Health, Sardar Vallabhai Patel
College of Veterinary and Animal Sciences, Meerut, Uttar Pradesh,
India;
shriyarawat@gmail.com
K. M.
Lokesh:
Division of Veterinary Public Health, Guru Angad Dev Veterinary
and Animal Sciences University, Ludhiana, Punjab, India;
lokeshvph@gmail.com
H. V.
Mohan:
Department of Veterinary Public Health, Veterinary College, Bidar,
Karnataka, India;
mohanhv@gmail.com
D.
Avinash Reddy:
Division of Veterinary Public Health, Indian Veterinary Research
Institute, Bareilly, Uttar Pradesh, India;
avi.incredible@gmail.com
Ashok
Kumar:
Division of Veterinary Public Health, Indian Veterinary
Research Institute, Bareilly, Uttar Pradesh, India;
ashokakt@rediffmail.com
K. N.
Bhilegaonkar:
Division of Veterinary Public Health, Indian Veterinary
Research Institute, Bareilly, Uttar Pradesh, India;
kiranvph@rediffmail.com
Received:
21-06-2014, Revised: 26-08-2014, Accepted: 02-09-2014, Published
online: 14-10-2014
Corresponding author:
Vysakh Mohan, e-mail: vysakhmohanvet@gmail.com
Abstract
Aim:
To study the prevalence of Rotavirus
in shellfish from Southern Kerala.
Materials and Methods: The shellfish samples after processing
was concentrated using proteinase K. RNA was isolated from the
concentrated samples using phenol chloroform method. Rota viral
RNA was detected using reverse transcriptionpolymerase chain
reaction.
Results: A low prevalence of 2.5% (5/200) was obtained during
the study. Rotavirus was detected in 2 out of 60 brown
mussels (3.33%), 2 out of 70 yellow clams (2.86%) and 1 out of 70
black clams (1.43%).
Conclusion: Low prevalence of Rotavirus
was obtained in our study. A more extensive study need to be
conducted to estimate the prevalence of enteric virus in
shellfish.
Keywords: concentration, proteinase K,
reverse transcription-polymerase chain reaction, Rotavirus,
shellfish.
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