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Research
(Published
online: 05-10-2014)
2.
Isolation and polymerase chain
reaction-based identification of Riemerella anatipestifer
from ducks in Kerala, India -
Manju Soman, Sreeja R.
Nair, M. Mini, Binu K. Mani and Siju Joseph
Veterinary World, 7(10): 765-769
doi:
10.14202/vetworld.2014.765-769
Manju
Soman:
Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences, Mannuthy, Thrissur, Kerala, India;
manjuso1993@gmail.com
Sreeja
R. Nair:
Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences, Mannuthy, Thrissur, Kerala, India;
drsreejarnair@gmail.com
M.
Mini:
Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences, Mannuthy, Thrissur, Kerala, India;
drmmini@yahoo.co.in
Binu K.
Mani:
Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences, Mannuthy, Thrissur, Kerala, India;
binukmani@yahoo.com
Siju
Joseph: Department of Veterinary Microbiology, College of
Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India;
siju96@gmail.com
Received:
29-06-2014, Revised: 27-08-2014, Accepted: 01-09-2014, Published
online: 05-10-2014
Corresponding author:
Manju Soman, e-mail: manjuso1993@gmail.com
Abstract
Aim:
The aim was to isolate and characterize Riemerella
anatipestifer organisms from disease outbreaks in ducks in
Kerala.
Materials and Methods: Ducklings, suspected of Riemerella
infection, were sacrificed and subjected to post-mortem
examination. Heart blood smears and impression smears from liver
and spleen were examined for the presence of pathogenic organisms.
Heart blood, lung, liver, and spleen collected aseptically from
the birds were subjected to isolation trials in brain heart
infusion agar and 10% bovine blood agar. The isolates were
characterized based on morphology, cultural characteristics and
biochemical tests, and their identity were confirmed by polymerase
chain reaction (PCR) and the PCR amplified DNA was sequenced. The
antibiotic sensitivity testing of the isolates were carried out
using six antibiotics viz ciprofloxacin, chloramphenicol,
enrofloxacin, amoxycillin, cotrimoxazole, and gentamicin.
Results: Colonies suggestive of Riemerella organisms
could be isolated on blood agar. Biochemical characterization and
PCR confirmed the identity of isolates as R. anatipestifer.
The nucleotide sequence of the PCR product showed 99% homology to
the R. anatipestifer sequences in the NCBI. The antibiogram
revealed that the organisms were sensitive to ciprofloxacin,
enrofloxacin, and gentamicin.
Conclusion: The present study suggests that the PCR assay can
facilitate fast and proper identification of R. anatipestifer
infection in ducks. The assay can also differentiate between
R. anatipestifer and Pasteurella multocida and can
replace the traditional methods of differentiation which are
cumbersome and time-consuming.
Keywords: antibiogram, ducks, isolation, polymerase chain
reaction, Riemerella anatipestifer.
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