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R esearch
(Published
online: 26-10-2014)
21.
Chromosome analysis of arsenic affected
cattle - S. Shekhar, A. K. Sahoo, N. Dalai, P.
Chaudhary, P. K. Praveen, R. Saikhom and R. Rai
Veterinary World, 7(10): 859-862
doi:
10.14202/vetworld.2014.859-862
S.
Shekhar:
Department of Animal Genetics and Breeding, West Bengal University
of Animal and Fishery Sciences, Kolkata, West Bengal, India;
sshekhar03@rediffmail.com
A. K.
Sahoo:
Department of Animal Genetics and Breeding, West Bengal University
of Animal and Fishery Sciences, Kolkata, West Bengal, India;
profaksahoo@gmail.com
N.
Dalai:
Department of Veterinary Physiology and Biochemistry, West Bengal
University of Animal and Fishery Sciences, Kolkata, West Bengal,
India;
niru.vets@gmail.com
P.
Chaudhary:
Department of Animal Genetics and Breeding, West Bengal University
of Animal and Fishery Sciences, Kolkata, West Bengal, India;
pankaj.choudhury@ymail.com
P. K.
Praveen:
Department of Veterinary Public Health and Epidemiology, West
Bengal University of Animal and Fishery Sciences, Kolkata, West
Bengal, India;
drpraveenvet2005@gmail.com
R.
Saikhom:
Department of Animal Genetics and Breeding, West Bengal University
of Animal and Fishery Sciences, Kolkata, West Bengal, India;
reshmasaikhom@gmail.com
R. Rai:
Department of Livestock Product and Technology, West Bengal
University of Animal and Fishery Sciences, Kolkata, West Bengal,
India;
drricharock85@gmail.com
Received:
11-07-2014, Revised: 16-09-2014, Accepted: 22-09-2014, Published
online: 23-10-2014
Corresponding author:
S. Shekhar, e-mail: sshekhar03@rediffmail.com
Abstract
Aim:
The aim was to study the chromosome analysis of arsenic
affected cattle.
Materials and Methods: 27 female cattle (21 arsenic affected
and 6 normal) were selected for cytogenetical study. The blood
samples were collected, incubated, and cultured using appropriate
media and specific methods. The samples were analyzed for
chromosome number and morphology, relative length of the
chromosome, arm ratio, and centromere index of X chromosome and
chromosomal abnormalities in arsenic affected cattle to that of
normal ones.
Results: The diploid number of metaphase chromosomes in
arsenic affected cattle as well as in normal cattle were all
2n=60, 58 being autosomes and 2 being sex chromosomes. From the
centromeric position, karyotyping studies revealed that all the 29
pair of autosomes was found to be acrocentric or telocentric, and
the sex chromosomes (XX) were submetacentric in both normal and
arsenic affected cattle. The relative length of all the autosome
pairs and sex chrosomosome pair was found to be higher in normal
than that of arsenic affected cattle. The mean arm ratio of
X-chromosome was higher in normal than that of arsenic affected
cattle, but it is reverse in case of centromere index value of
X-chromosome. There was no significant difference of arm ratio and
centromere index of X-chromosomes between arsenic affected and
normal cattle. No chromosomal abnormalities were found in arsenic
affected cattle.
Conclusion: The chromosome analysis of arsenic affected cattle
in West Bengal reported for the first time in this present study
which may serve as a guideline for future studies in other
species. These reference values will also help in comparison of
cytological studies of arsenic affected cattle to that of various
toxicants.
Keywords: arsenic, autosomes, karyotyping, metaphase
chromosome.
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