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Research
(Published
online: 31-10-2014)
27. Biochemical characterization of
preovulatory and cystic ovarian follicular fluid of sow -
R. Nath, N. Mahanta, M. Islam and S. S. Deka
Veterinary World, 7(10): 895-898
doi:
10.14202/vetworld.2014.895-898
R. Nath:
Department of
Veterinary Biochemistry, College of Veterinary Science, Guwahati,
Assam, India;
ritasankr@rediffmail.com
N.
Mahanta:
Department of Animal Reproduction, Gynaecology and Obstetrics,
College of Veterinary Science, Guwahati, Assam, India;
nipendramahanta@gmail.com
M.
Islam: Department
of Veterinary Pathology, College of Veterinary Science, Guwahati,
Assam, India;
muzaharulislam@gmail.com
S. S.
Deka: Department
of Veterinary Biochemistry, College of Veterinary Science,
Guwahati, Assam, India;
surakshasubedi@gmail.com
Received:
01-07-2014, Revised:
29-09-2014, Accepted:
04-10-2014, Published online:
31-10-2014
Corresponding author:
Rita Nath, e-mail: ritasankr@rediffmail.com
Abstract
Aim:
The present study was designed to biochemically characterize
ovarian cyst follicular fluid of sows and to relate possible
changes in relation to preovulatory ovarian follicular fluid of
sows.
Materials and Methods: Ovaries were collected from adult and
non-pregnant slaughtered sow (Hampshire X local cross). A total of
16 pairs of ovaries were investigated, and they were classified
according to their pathological and physiological status into two
categories i.e. preovulatory and cystic follicle (diameter >25 mm
with turbid appearance). Fluid was aspirated from the follicles
and stored at −20°C prior to assaying. Follicular fluid samples
were analyzed for glucose, total protein, cholesterol,
triglycerides, blood urea nitrogen (BUN), urea, creatinine, uric
acid. The activities of the enzymes aspartate transaminase,
alanine transaminase (ALT), alkaline phosphatase (ALP), creatine
kinase (CK) and gamma-glutamyl transpeptidase (GGT) were also
estimated by spectrophotometric methods. The data were
statistically analyzed using the SPSS software.
Results: As follicles became cystic, the concentrations of
glucose, cholesterol, triglyceride, total protein and albumin
showed a significant (p<0.05) increased while BUN decreased
significantly (p<0.05). The enzyme activities of ALT, ALP and CK
increased significantly (p<0.05), but the activity of GGT
significantly (p<0.05) decreased.
Conclusion: Results obtained showed that there was a
significant change in most of the cystic follicular fluid
metabolites.
Keywords: biochemical characterization,
follicular fluid, sows.
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