Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916

ISSN (Print): 0972-8988


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License

( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online: 31-10-2014)

27. Biochemical characterization of preovulatory and cystic ovarian follicular fluid of sow - R. Nath, N. Mahanta, M. Islam and S. S. Deka

Veterinary World, 7(10): 895-898



   doi: 10.14202/vetworld.2014.895-898



R. Nath: Department of Veterinary Biochemistry, College of Veterinary Science, Guwahati, Assam, India;

N. Mahanta: Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science, Guwahati, Assam, India;

M. Islam: Department of Veterinary Pathology, College of Veterinary Science, Guwahati, Assam, India;

S. S. Deka: Department of Veterinary Biochemistry, College of Veterinary Science, Guwahati, Assam, India;


Received: 01-07-2014, Revised: 29-09-2014, Accepted: 04-10-2014, Published online: 31-10-2014


Corresponding author: Rita Nath, e-mail:

Aim: The present study was designed to biochemically characterize ovarian cyst follicular fluid of sows and to relate possible changes in relation to preovulatory ovarian follicular fluid of sows.

Materials and Methods: Ovaries were collected from adult and non-pregnant slaughtered sow (Hampshire X local cross). A total of 16 pairs of ovaries were investigated, and they were classified according to their pathological and physiological status into two categories i.e. preovulatory and cystic follicle (diameter >25 mm with turbid appearance). Fluid was aspirated from the follicles and stored at −20C prior to assaying. Follicular fluid samples were analyzed for glucose, total protein, cholesterol, triglycerides, blood urea nitrogen (BUN), urea, creatinine, uric acid. The activities of the enzymes aspartate transaminase, alanine transaminase (ALT), alkaline phosphatase (ALP), creatine kinase (CK) and gamma-glutamyl transpeptidase (GGT) were also estimated by spectrophotometric methods. The data were statistically analyzed using the SPSS software.

Results: As follicles became cystic, the concentrations of glucose, cholesterol, triglyceride, total protein and albumin showed a significant (p<0.05) increased while BUN decreased significantly (p<0.05). The enzyme activities of ALT, ALP and CK increased significantly (p<0.05), but the activity of GGT significantly (p<0.05) decreased.

Conclusion: Results obtained showed that there was a significant change in most of the cystic follicular fluid metabolites.

Keywords: biochemical characterization, follicular fluid, sows.

1. Tal, R.B., Pen, S. and Roth, Z. (2009) Ovarian cyst in high yielding dairy cow. Theriogenology, 2: 690-698.
2. Gerard, N., Loiseau, S., Duchamp, G. and Seguin, G. F. (2002) Analysis of the variations of follicular fluid composition during follicular growth and maturation in the mare using proton nuclear magnetic resonance (1H NMR). Reproduction, 124: 241-248.
3. Maniwa, J., Izumi, S., Isobe, N. and Terada, T. (2005) Studies on substantially increased proteins in follicular fluid of bovine ovarian follicular cysts using 2-D PAGE and MALDITOF MS. Reprod. Biol. Endocrin., 3: 23.
PMid:15941490 PMCid:PMC1177988
4. Gode, F., Gulekli, B. Dogan, E., Korhan, P., Dogan, S., Bige, O., Cimrin, D. and Atabey, N. (2011) Influence of follicular fluid GDF9 and BMP15 on embryo quality. Fertil. Steril., 95: 2274-2278.
5. Nath, R., Das, S., Sarma, S. and Devi, M. (2014) Comparison of blood profiles between healthy and Brucella affected cattle. Vet. World, 7(9): 668-670.
6. Khan, F.A., Das, G.K., Pande, M., Mir, R.A. and Shankar, U. (2011) Changes in biochemical composition of follicular fluid during reproductive acyclicity in water buffalo (Bubalus bubalis). Anim. Reprod. Sci., 127: 38-42.
7. Garm, O. (1949) A study on bovine nymphomania with special reference to etiology and pathogenesis. Acta Endocrinol., 3: 1-141.
8. Khan, F.A. and Das, G.K. (2012) Follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in water buffalo (Bubalus bubalis). Trop. Anim. Health Prod., 44: 125-131.
9. Rizzo, A., Cosala, S. and Sciorsci, R.L. (2010) Bovarian ovarian follicular cyst in vitro effecys of lecirilin, a GnRH analogue. Theriogenology, 74: 1559-1569.
10. Zeitoun, M.M. (2012) Effect of physiological status of buffalo ovaries on progesterone and biochemical constituents in follicular fluids. Proceedings of the 26th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Egypt.
11. Cynthia, J. and Johnson, M.S. (2006) Cystic ovarian disease in cattle on dairies in central and Western Ohio: Ultrasonic, hormonal, histologic, and metabolic assessments. Thesis Submitted to School of the Ohio State University.
12. Hassanin, K.D. (2007) Biochemical and hormonal changes in the follicular fluid in case of cystic ovarian diseases in cows. Res. Appl. Act., 54: 98-95.
13. Ahmed, W.H., Shalaby, S.I.A. and Zaabal, M.M. (1997) Some biochemical constituents of preovulatory and cystic ovarian follicular fluids in buffalo cows with emphasis on protein polymorphism. Buffalo Bull., 16(1): 5-10.
14. Carona, F.F.N., Olssan, J.H. and Hillensjo, T. (1983) Lipids, lipoprotein and steroids in serum and in fluid from stimulated and non Stimulated human ovarian follicule. Endocrinology, 111: 558-562.
15. Kalmath, G.P. and Ravindra, J.P. (2007) Mineral profiles of ovarian antral follicular fluid in buffaloes during follicular development. Indian. J. Anim. Res., 41(2): 87-93.
16. Abd Ellah, M.R., Hussein, H.A. and Derar, D.R. (2010) Ovarian follicular fluid constituents in relation to stage of estrus cycle and size of the follicle in buffalo. Vet. World, 3(6): 263-267.
17. Albomohsen, H., Mamouei, M., Tabatabaei, S. and Fayazi, J. (2011) Metabolite composition variations of follicular fluid and blood serum in Iranian dromedary camels during the peak breeding season. J. Anim. Vet. Adv., 10(3):327-331.