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Research
(Published
online: 09-10-2014)
7.
Hematology and serum biochemistry of captive
gharial (Gavialis gangeticus) in India -
Shahnaz Amin and Avadh
Bihari Shrivastav
Veterinary World, 7(10): 794-798
doi:
10.14202/vetworld.2014.794-798
Shahnaz
Amin:
Centre for Wildlife Forensics and Health, Nanaji Deshmukh
Veterinary Science University, South Civil Lines, Jabalpur, Madhya
Pradesh, India;
vetsamin@live.com
Avadh
Bihari Shrivastav: Centre for Wildlife Forensics and Health,
Nanaji Deshmukh Veterinary Science University, South Civil Lines,
Jabalpur, Madhya Pradesh, India;
drabshrivastav@gmail.com
Received:
31-05-2014, Revised: 02-09-2014, Accepted: 07-09-2014, Published
online: 09-10-2014
Corresponding author:
Shahnaz Amin, e-mail: vetsamin@live.com
Abstract
Aim:
To study the hematological and serum biochemical parameters of
the critically endangered gharial (Gavialis gangeticus).
Materials and Methods: Blood samples for hemato-biochemical
analyses were collected from the ventral median coccygeal vein of
six juvenile and six sub adult gharials of Dewari Gharial Rearing
Centre of National Chambal Sanctuary, Madhya Pradesh, India.
Hematological examination was performed manually. Differential
leukocyte count was performed on the blood smears stained with
Giemsa’s stain. The analysis of serum was conducted by eppendorf
ECOM-F 6124 semi auto biochemical analyzer using standard ERBA
biochemical reagent kits.
Results: Peripheral blood cells of gharial showed erythrocytes
with an oval outline and centrally located prominent round to oval
nucleus. Erythrocyte count in sub adult gharials was significantly
greater than juveniles. Whereas erythrocyte mean corpuscular
volume and erythrocyte size in juveniles was significantly larger
than sub adults. The average most abundant leukocyte type in
gharial was lymphocytes (53%), followed by heterophils (27%),
eosinophils (10%), monocytes (7%) and basophils (3%). Aspartate
aminotransferase, alkaline phosphatase, blood urea nitrogen,
triglycerides and albumin concentrations in sub adult gharials
were significantly higher than juveniles. No significant
differences were determined in other hemato-biochemical parameters
between juvenile and sub adult gharials under study.
Conclusion: A preliminary database on hematology and blood
biochemistry of gharial was established. The data will be useful
in routine health evaluations, especially in relation to
determining potential effects associated with factors such as
pollution and infectious diseases.
Keywords: crocodilians, gharial, Gavialis gangeticus,
hematobiochemistry, National Chambal Sanctuary.
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