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Research
(Published
online: 02-09-2014)
1.
Detection of tetracycline resistance
determinant tetA gene and antimicrobial resistance pattern
in Escherichia coli isolates recovered from healthy layer
chickens -
A. Balasubramaniam, M.
Arthanari Eswaran, P. Suresh and K. Sukumar
Veterinary World, 7(9): 635-638
doi:
10.14202/vetworld.2014.635-638
A. Balasubramaniam: Department of
Veterinary Microbiology, Veterinary College and Research
Institute, Namakkal, Tamil Nadu, India;
balasubramaniam72@gmail.com
M. Arthanari Eswaran: Department of
Veterinary Microbiology, Veterinary College and Research
Institute, Namakkal, Tamil Nadu, India;
drarthu@gmail.com
P. Suresh: Department of Veterinary
Microbiology, Veterinary College and Research Institute, Namakkal,
Tamil Nadu, India; vet_suresh@yahoo.com
K. Sukumar: Department of Veterinary
Microbiology, Veterinary College and Research Institute, Namakkal,
Tamil Nadu, India; drksugu@gmail.com
Received: 06-05-2014, Revised: 02-07-2014,
Accepted: 12-07-2014, Published online: 02-09-2014
Corresponding author: A.
Balasubramaniam, email: balasubramaniam72@gmail.com
Abstract
Aim:
The aim was to study the occurrence of tetracycline resistance
determinant tetA gene, and antibacterial resistance pattern
in commensal Escherichia coli recovered from healthy
non-clinical layer chickens.
Materials and Methods: Twenty-four cloacal swabs were obtained
from 15 flocks in five different layer farms located at around
Namakkal, which is a place of high-intensity layer chicken rearing
in south India. Identification of E. coli was carried out
by performing cultural and biochemical tests. Antimicrobial
resistance test was carried out using disc diffusion method. The
polymerase chain reaction employing tetAC forward and
tetAC reverse primers were carried out to detect tetA
gene conferring resistance to tetracycline.
Result:
All the collected cloacal swabs yielded E. coli.
Twenty-one isolates (88%) were resistant to tetracycline
antibiotic in disc diffusion method. All the isolates showed
resistance to more than six antibiotics, which implied existence
of multidrug resistant microbiota in intestine of poultry. Only
seven (29%) isolates showed the presence of tetA gene
indicating the involvement of either other one or more efflux
gene(s), namely tetB, tetC and tetD or
ribosomal protection encoded by tetM, tetO, tetQ
and tetS genes than tetA gene.
Conclusion: Based on the presence of tetA gene among
tetracycline-resistant bacteria in healthy non-clinical
food-producing animals such as layer chickens, it can be
significant in human medicine as tetA gene could easily be
spread to other bacteria. This kind of phenomenon can be
extrapolated in transfer of resistance for other antibacterial
essential for treating bacterial infections in human. We conclude
that preventing the spread of antimicrobial resistance through
direct or indirect contact, consumed food/feed and through the
environment is empirical in reduction of failures while treating
bacterial infections.
Keywords: antibacterial
resistance, Escherichia coli, tetA gene, tetracycline.
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