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Research (Published online: 20-09-2014)

15. Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio - J. S. Rajoriya, J. K. Prasad, S. K. Ghosh, Snehal S. Ramteke, N. C. Barik, G. K. Das and Megha Pande

Veterinary World, 7(9): 702-706



   doi: 10.14202/vetworld.2014.702-706



J. S. Rajoriya: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India;

J. K. Prasad: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India;

S. K. Ghosh: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India;

Snehal S. Ramteke: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India;

N. C. Barik: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India;

G. K. Das: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly,

Uttar Pradesh, India;

Megha Pande: Animal Physiology Lab, Central Institute for Research on Cattle, Meerut Cantt, Uttar Pradesh, India;


Received: 24-05-2014, Revised: 02-08-2014, Accepted: 06-08-2014, Published online: 20-09-2014


Corresponding author: J. S. Rajoriya, e-mail:

Aim: The study was conducted to investigate the effect of cholesterol loaded cyclodextrin (CLC) on freezability of buffalo spermatozoa.

Materials and Methods: Murrah buffalo bull semen samples with progressive motility of 70% and greater were used. After the evaluation of motility and livability, four equal fractions of semen samples were made. Group I was kept as control and diluted with Tris, whereas Group II, III and IV were treated with CLC solution at the rate of 2.0, 3.0 and 4.0 mg/ml respectively to obtain 120 106 sperm/ml as final spermatozoa concentration. The aliquots of all the groups were incubated for action of CLC, followed by dilution and freezing. Evaluation at pre-freeze and post-thaw stage of progressive motility, viability and level of cholesterol and phospholipid was done.

Results: The mean cholesterol content (μg/100 106 spermatozoa) of Group I, II, III and IV at pre-freeze stage was 21.550.63, 49.561.38, 55.670.45 and 47.791.01 and at post-thaw stage were 13.180.45, 34.270.71, 36.210.48 and 33.680.56, respectively. At pre-freeze stage, cholesterol content was significantly (p<0.01) higher in Group III in comparison to other groups. The mean cholesterol and phospholipids content of fresh sperm was 24.140.58 and 51.130.66 μg/100 106 sperm cells, respectively, and C/P ratio of spermatozoa at fresh stage was 0.470.067.

Conclusion: CLC treatment maintains the C/P ratio and plays an important role in maintaining membrane architecture of spermatozoa. Hence, addition of CLC may be helpful in increasing freezability of buffalo spermatozoa by increasing the C/P ratio of spermatozoa.

Keywords: buffalo, cholesterol loaded cyclodextrin, cholesterol:phospholipid (C/P) ratio.

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