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Research
(Published
online: 20-09-2014)
15.
Cholesterol loaded cyclodextrin increases freezability of buffalo
bull (Bubalus bubalis) spermatozoa by increasing
cholesterol to phospholipid ratio -
J. S. Rajoriya, J. K.
Prasad, S. K. Ghosh, Snehal S. Ramteke, N. C. Barik, G. K. Das and
Megha Pande
Veterinary World, 7(9): 702-706
doi:
10.14202/vetworld.2014.702-706
J. S.
Rajoriya: Germ Plasm Centre, Division of Animal Reproduction,
Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar
Pradesh, India;
jituivri@gmail.com
J. K.
Prasad: Germ Plasm Centre, Division of Animal Reproduction,
Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar
Pradesh, India;
jkprasad2001@yahoo.com
S. K.
Ghosh: Germ Plasm Centre, Division of Animal Reproduction,
Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar
Pradesh, India;
subrata_kghosh@yahoo.co.in
Snehal
S. Ramteke: Germ Plasm Centre, Division of Animal
Reproduction, Indian Veterinary Research Institute, Izatnagar,
Bareilly,
Uttar
Pradesh, India;
drsnehalramteke@rediffmail.com
N. C.
Barik: Germ Plasm Centre, Division of Animal Reproduction,
Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar
Pradesh, India;
iamdrncbarik@gmail.com
G. K.
Das: Germ Plasm Centre, Division of Animal Reproduction,
Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar
Pradesh, India;
gkdasivri@gmail.com
Megha
Pande: Animal Physiology Lab, Central Institute for Research
on Cattle, Meerut Cantt, Uttar Pradesh, India;
megha985@gmail.com
Received: 24-05-2014,
Revised:
02-08-2014,
Accepted:
06-08-2014,
Published online:
20-09-2014
Corresponding author:
J. S. Rajoriya, e-mail: jituivri@gmail.com
Abstract
Aim:
The study was conducted to investigate the effect of
cholesterol loaded cyclodextrin (CLC) on freezability of buffalo
spermatozoa.
Materials and Methods: Murrah buffalo bull semen samples with
progressive motility of 70% and greater were used. After the
evaluation of motility and livability, four equal fractions of
semen samples were made. Group I was kept as control and diluted
with Tris, whereas Group II, III and IV were treated with CLC
solution at the rate of 2.0, 3.0 and 4.0 mg/ml respectively to
obtain 120 × 106 sperm/ml as final spermatozoa
concentration. The aliquots of all the groups were incubated for
action of CLC, followed by dilution and freezing. Evaluation at
pre-freeze and post-thaw stage of progressive motility, viability
and level of cholesterol and phospholipid was done.
Results: The mean cholesterol content (μg/100 × 10 6
spermatozoa) of Group I, II, III and IV at
pre-freeze stage was 21.55±0.63, 49.56±1.38, 55.67±0.45 and
47.79±1.01 and at post-thaw stage were 13.18±0.45, 34.27±0.71,
36.21±0.48 and 33.68±0.56, respectively. At pre-freeze stage,
cholesterol content was significantly (p<0.01) higher in Group III
in comparison to other groups. The mean cholesterol and
phospholipids content of fresh sperm was 24.14±0.58 and 51.13±0.66
μg/100 × 106 sperm cells, respectively, and
C/P ratio of spermatozoa at fresh stage was 0.47±0.067.
Conclusion: CLC treatment maintains the C/P
ratio and plays an important role in maintaining membrane
architecture of spermatozoa. Hence, addition of CLC may be helpful
in increasing freezability of buffalo spermatozoa by increasing
the C/P ratio of spermatozoa.
Keywords: buffalo, cholesterol loaded
cyclodextrin, cholesterol:phospholipid (C/P) ratio.
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