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Research
(Published
online: 20-09-2014)
16.
Comparative study of heparin-binding proteins profile of Murrah
buffalo (Bubalus bubalis) semen -
S. S. Ramteke, A. M.
Shende,
J. S. Rajoriya, N. C.
Barik, Megha Pande, S. K. Bhure, G. K. Das, S. K. Ghosh and J. K.
Prasad
Veterinary World, 7(9): 707-711
doi:
10.14202/vetworld.2014.707-711
S.
S. Ramteke: Germ Plasm Centre, Division of Animal
Reproduction, Indian Veterinary Research Institute, Izatnagar,
Bareilly, Uttar Pradesh, India;
drsnehalramteke@rediffmail.com
A.
M. Shende: Protein Research Laboratory, Animal Biochemistry
Division, Indian Veterinary Research Institute, Izatnagar,
Bareilly, Uttar Pradesh, India;
asashishivri216@gmail.com
J.
S. Rajoriya: Germ Plasm Centre, Division of Animal
Reproduction, Indian Veterinary Research Institute, Izatnagar,
Bareilly, Uttar Pradesh, India;
jituivri@gmail.com
N. C. Barik: Germ Plasm Centre, Division of Animal
Reproduction, Indian Veterinary Research Institute, Izatnagar,
Bareilly, Uttar Pradesh, India;
iamdrncbarik@gmail.com
Megha Pande: Animal Physiology Lab, Central Institute for
Research on Cattle, Meerut Cantt, Uttar Pradesh, India;
megha985@gmail.com
S. K. Bhure: Protein Research Laboratory, Animal
Biochemistry Division, Indian Veterinary Research Institute,
Izatnagar, Bareilly, Uttar Pradesh, India;
sdbhure@rediffmail.com
G. K. Das: Germ Plasm Centre, Division of Animal
Reproduction, Indian Veterinary Research Institute, Izatnagar,
Bareilly, Uttar Pradesh, India;
gkdasivri@gmail.com
S.
K. Ghosh: Germ Plasm Centre, Division of Animal Reproduction,
Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar
Pradesh, India;
subrata_kghosh@yahoo.co.in
J.
K. Prasad: Germ Plasm Centre, Division of Animal Reproduction,
Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar
Pradesh, India;
jkprasad2001@yahoo.co.in
Received:
12-06-2014,
Revised:
31-07-2014,
Accepted:
06-08-2014,
Published online:
20-09-2014
Corresponding author:
S. S. Ramteke, e-mail:
drsnehalramteke@rediffmail.com
Abstract
Aim:
The experiment was conducted to study the total seminal plasma
protein (TSPP) and heparin-binding proteins (HBPs) in relation to
initial semen quality of buffalo bull.
Materials and Methods: Semen from two Murrah buffalo bulls
(bull no. 605 and 790) with mass motility of
≥3+ were used for
the study and categorized into three groups (Group I- Mass
motility 3+, Group II- Mass motility 4+ and Group III- Mass
motility 5+). Seminal plasma from semen was separated by
centrifugation. HBPs was isolated and purified from heparin-agarose
affinity column by modified elution buffer. TSPP and isolated HBPs
concentration was estimated by Lowry’s method. The purified HBPs
were resolved on Sodium dodecyl sulfate polyacrylamide gel
electrophoresis to check the protein profile of two bulls.
Results: The mean values of TSPP
concentrations in bull no. 605 and 790 in Group I, II and III were
30.64±0.12, 31.66±0.09, 32.53±0.19 and 28.51±0.09, 29.49±0.15,
30.45±0.17 mg/mL, respectively. The mean values of HBPs
concentrations in bull no. 605 and 790 in Group I, II and III were
3.11±0.07, 3.32±0.06, 3.46±0.08 and 2.51±0.08, 2.91±0.05,
3.10±0.03 mg/mL, respectively. Both the values of TSPP and HBPs
were significantly higher (p<0.01) in bull no. 605 when compared
to 790 in all the three groups. 31 kDa HBP was more intensely
present in bull no. 605, thus may indicate its superiority over
bull no. 790 in relation to fertility potential.
Conclusion: TSPP and HBPs shows variation in
concentration with respect to initial semen quality. Furthermore,
presence of fertility related 31 kDa HBPs in one of the bull may
be an indication of high fertility of a bull. In future,
in-vivo and in-vitro correlative study on larger basis
is needed for the establishment of fertility-related HBPs in semen
which might establish criteria for selection of buffalo bull with
high fertility potential.
Keywords: buffalo bull, heparin-binding
proteins, semen, seminal plasma.
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