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Research (Published online: 20-09-2014)

16. Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen - S. S. Ramteke, A. M. Shende,

J. S. Rajoriya, N. C. Barik, Megha Pande, S. K. Bhure, G. K. Das, S. K. Ghosh and J. K. Prasad

Veterinary World, 7(9): 707-711

 

 

   doi: 10.14202/vetworld.2014.707-711

 

 

S. S. Ramteke: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; drsnehalramteke@rediffmail.com

A. M. Shende: Protein Research Laboratory, Animal Biochemistry Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; asashishivri216@gmail.com

J. S. Rajoriya: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; jituivri@gmail.com

N. C. Barik: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; iamdrncbarik@gmail.com

Megha Pande: Animal Physiology Lab, Central Institute for Research on Cattle, Meerut Cantt, Uttar Pradesh, India; megha985@gmail.com

S. K. Bhure: Protein Research Laboratory, Animal Biochemistry Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; sdbhure@rediffmail.com

G. K. Das: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; gkdasivri@gmail.com

S. K. Ghosh: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; subrata_kghosh@yahoo.co.in

J. K. Prasad: Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India; jkprasad2001@yahoo.co.in

 

Received: 12-06-2014, Revised: 31-07-2014, Accepted: 06-08-2014, Published online: 20-09-2014

 

Corresponding author: S. S. Ramteke, e-mail: drsnehalramteke@rediffmail.com



Aim: The experiment was conducted to study the total seminal plasma protein (TSPP) and heparin-binding proteins (HBPs) in relation to initial semen quality of buffalo bull.

Materials and Methods: Semen from two Murrah buffalo bulls (bull no. 605 and 790) with mass motility of 3+ were used for the study and categorized into three groups (Group I- Mass motility 3+, Group II- Mass motility 4+ and Group III- Mass motility 5+). Seminal plasma from semen was separated by centrifugation. HBPs was isolated and purified from heparin-agarose affinity column by modified elution buffer. TSPP and isolated HBPs concentration was estimated by Lowry’s method. The purified HBPs were resolved on Sodium dodecyl sulfate polyacrylamide gel electrophoresis to check the protein profile of two bulls.

Results: The mean values of TSPP concentrations in bull no. 605 and 790 in Group I, II and III were 30.64±0.12, 31.66±0.09, 32.53±0.19 and 28.51±0.09, 29.49±0.15, 30.45±0.17 mg/mL, respectively. The mean values of HBPs concentrations in bull no. 605 and 790 in Group I, II and III were 3.11±0.07, 3.32±0.06, 3.46±0.08 and 2.51±0.08, 2.91±0.05, 3.10±0.03 mg/mL, respectively. Both the values of TSPP and HBPs were significantly higher (p<0.01) in bull no. 605 when compared to 790 in all the three groups. 31 kDa HBP was more intensely present in bull no. 605, thus may indicate its superiority over bull no. 790 in relation to fertility potential.

Conclusion: TSPP and HBPs shows variation in concentration with respect to initial semen quality. Furthermore, presence of fertility related 31 kDa HBPs in one of the bull may be an indication of high fertility of a bull. In future, in-vivo and in-vitro correlative study on larger basis is needed for the establishment of fertility-related HBPs in semen which might establish criteria for selection of buffalo bull with high fertility potential.

Keywords: buffalo bull, heparin-binding proteins, semen, seminal plasma.



1. Killian, G.J., Chapman, D.A. and Rogowski, L.A. (1993) Fertility associated proteins in Holstein bull seminal plasma. Biol. Reprod., 49(6): 1202-1207.
http://dx.doi.org/10.1095/biolreprod49.6.1202
PMid:8286602
 
2. Calvete, J.J., Raida, M., Gentzel, M., Urbanke, C., Sanz, L. and Topfer-Petersen, E. (1997) Isolation and characterization of heparin - And phosphorylcholine-binding proteins of boar and stallion seminal plasma: primary structure of porcine pB1. FEBS Lett., 407(2): 201-206.
http://dx.doi.org/10.1016/S0014-5793(97)00344-X
 
3. Villemure, M., Lazure, C. and Manjunath, P. (2003) Isolation and characterization of gelatin-binding proteins from goat seminal plasma. Reprod. Biol. Endocrinol., 1: 39.
http://dx.doi.org/10.1186/1477-7827-1-39
PMid:12737634 PMCid:PMC155548
 
4. Jobim, M.I.M., Oberst E.R., Salbego C.G., Wald, V.B., Horn A.P., and Mattos R.C. (2005) BSP A1/A2-like proteins in ram seminal plasma. Theriogenology, 63(7): 2053-2062.
http://dx.doi.org/10.1016/j.theriogenology.2004.10.002
PMid:15823360
 
5. Martınez-Heredia, J., de Mateo, S., Vidal-Taboada, J.M., Ballesca, J.L. and Oliva, R. (2008) Identification of proteomic differences in asthenozoospermic sperm samples. Hum. Reprod., 23(4): 783-791.
http://dx.doi.org/10.1093/humrep/den024
PMid:18281682
 
6. Manjunath, P., Bergeron, A., Lefebvre, J., and Ton, J. (2008) Seminal plasma proteins: Function and interaction with protective agents during semen preservation. Biochem. Biophy. Acta., 1784: 891-899.
 
7. Manjunath, P., Lefebvre, J., Jois P.S., Fan, J. and Wright, M.W. (2009) New nomenclature for mammalian BSP genes. Biol. Reprod., 80(3): 394-397.
http://dx.doi.org/10.1095/biolreprod.108.074088
PMid:18923155 PMCid:PMC2844493
 
8. Miller, D.J., First, N.L. and Ax, R.L. (1987) Isolation and characterization of seminal fluid proteins that bind heparin. Adv. Exp. Med. Biol., 219: 597-601.
http://dx.doi.org/10.1007/978-1-4684-5395-9_27
PMid:3434444
 
9. Nass, S.J., Miller, D. J., Winer, M.A. and Ax, R.L. (1990) Male accessory sex glands produce heparin-binding proteins that bind to cauda epididymal spermatozoa and are testosterone dependent. Mol. Reprod. Dev., 25(3): 237-246.
http://dx.doi.org/10.1002/mrd.1080250305
PMid:2331373
 
10. Calvete, J.J., Sanz, L., Reinert, M., Dostalova, Z. and Topfer-Petersen, E. (1995) Heparin binding proteins on bull, boar, stallion, and human spermatozoa. In: Jamieson, B.G.M., Ausio, J. and Justine J.L. editors. Advances in Spermatozoal Phylogeny and Taxonomy. Vol. 166. Memoires du Museum National D'Histoire Naturelle, Paris. p515-524.
 
11. Manjunath, P., Chandonnet, E., Leblond, E. and Desnoyers, S.L. (1993) Major proteins of bovine seminal plasma bind to spermatozoa. Biol. Reprod., 49: 27-37.
 
12. Bellin, M.E., Hawkins, H.E., Oyarzo, J.N., Vanderboom, R.J. and Ax, R.L. (1995) Monoclonal antibody detection of heparin-binding proteins on sperm corresponds to increased fertility of bulls. J. Anim. Sci., 74(1): 173-182.
 
13. Arangasamy, A., Singh, L.P., Ahmed, N., Ansari, M.R. and Ram, G.C. (2005) Isolation and characterization of heparin and gelatin binding buffalo seminal plasma proteins and their effect on cauda epididymal spermatozoa. Anim. Reprod. Sci., 90(3-4): 243-254.
http://dx.doi.org/10.1016/j.anireprosci.2004.12.014
PMid:16260100
 
14. Harshan, H.M. (2007) Modulation of structural and functional properties of buffalo cauda epididymal spermatozoa by sperm binding seminal plasma protein (PDC-109). Ph. D. Thesis Submitted to Deemed University IVRI, Izatnagar, U.P., India.
 
15. Singh, M. (2012) Isolation and purification of PDC-109 protein from buffalo seminal plasma and its relation with freez ability. M.V.Sc. Thesis Submitted to Deemed University IVRI, Izatnagar, U.P., India.
 
16. Arangasamy, A. (2003) Isolation of buffalo seminal plasma proteins and their effect on in vitro capacitation, acrosome reaction and fertilizing potential of spermatozoa. Ph. D. Thesis Submitted to Deemed University IVRI, Izatnagar, U.P., India.
 
17. Kumar, A. (2005) Effect of seminal plasma heparin binding and non-heparin binding proteins on the freezability and in vitro fertility of buffalo cauda spermatozoa. M.V.Sc. Thesis Submitted to Deemed University IVRI, Izatnagar, U.P., India.
 
18. Lowry, O.H., Rosenberg, N.J. Farr, A.L. and Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem., 193(1): 265-275.
PMid:14907713
 
19. Laemmli, V.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature., 227(5259): 660-685.
http://dx.doi.org/10.1038/227680a0
 
20. Kulkarni, B.A., Rupal, R.K. and Hegde, U.C. (1998) Comparative SDS-polyacrylamide gel electrophoresis of seminal plasma proteins and blood plasma proteins of the Indian buffalo and cattle bulls. Indian. J. Anim. Sci., 68: 66-67.
 
21. Rahman, M.S., Islam, M.S., Rahman, M.T., Parvez, N.H. and Rhaman, M.M. (2010) Morphometric analysis of vesicular glands of indigenous bull. Int. J. Sustain. Crop. Prod., 5: 11-14.
 
22. Nauc, V. and Manjunath, P. (2000) Radio immunoassays for bull seminal plasma proteins (BSP-A1/A2, BSP-A3, and BSP-30- kilo Daltons) and their quantification in seminal plasma and sperm. Biol. Reprod., 63(4): 1058-1066.
http://dx.doi.org/10.1095/biolreprod63.4.1058
PMid:10993827
 
23. Singh, M., Ghosh, S.K., Prasad, J.K., Kumar A., Tripathi, R.P., Bhure, S.K. and Srivastava, N. (2014) Seminal PDC-109 protein vis-à-vis cholesterol content and freezability of buffalo spermatozoa. Anim. Reprod. Sci., 144(1-2): 22-29.
http://dx.doi.org/10.1016/j.anireprosci.2013.10.016
PMid:24289867
 
24. Singh, M., Ghosh, S.K., Prasad, J.K., Kumar A., Ramteke, S.S., Srivastava, N. and Rajoriya J.S. (2014) Isolation, characterization and quantitation of heparin binding proteins and PDC-109 protein from buffalo bull semen. Indian. Vet. J., 91(2): 27-29.
 
25. Bellin, M.E., Oyarzo, J.N., Hawkins, H.E., Zhang, H.M., Smith, R.G., Forest, D.W., Sprott, L.R. and Ax, R.L. (1998) Fertility-associated antigen on bull sperm indicates fertility potential. J. Androl. Sci., 76(8): 2032-2039.
 
26. Shinde, S., Mahmood, S., Singh, G. and Verma, M.R. (2014) Association between the seminal vesicle weight and certain steroids in buffaloes (Bubalus bubalis). Vet. World., 7(1): 21-25.
http://dx.doi.org/10.14202/vetworld.2014.21-25