Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916

ISSN (Print): 0972-8988

 

Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ


Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License

(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.


Research (Published online: 30-09-2014)

22. Sensitivity, specificity, predictive value and accuracy of ultrasonography in pregnancy rate prediction in Sahelian goats after progesterone impregnated sponge synchronization - Justin Kouamo, Adama Sow, Miguiri Kalandi and Germain Jerome Sawadogo

Veterinary World, 7(9): 744-748

 

 

   doi: 10.14202/vetworld.2014.744-748

 

 

Justin Kouamo: Department of Surgery and Medical Pathology, School of Veterinary Medicine and Sciences, The University of Ngaoundere, Ngaoundere, Cameroon; justinkouamo@yahoo.fr

Adama Sow: Laboratory of Endocrinology and Radio-immunology, Service of Biological and Medical Physics and Chemistry, EISMV, Dakar, Senegal; wosamada@yahoo.fr

Miguiri Kalandi: Laboratory of Endocrinology and Radio-immunology, Service of Biological and Medical Physics and Chemistry, EISMV, Dakar, Senegal; migson77@yahoo.fr

Germain Jerome Sawadogo: Laboratory of Endocrinology and Radio-immunology, Service of Biological and Medical Physics and Chemistry, EISMV, Dakar, Senegal; sawadogo@refer.sn

 

Received: 02-05-2014, Revised: 01-08-2014, Accepted: 12-08-2014, Published online: 30-09-2014

 

Corresponding author: Justin Kouamo, e-mail: justinkouamo@yahoo.fr



Aim: This study was aimed to evaluate the sensitivity, specificity, predictive value and accuracy of ultrasonography in pregnancy rate (PR) prediction in Sahelian goats after progesterone impregnated sponge synchronization within the framework of caprine artificial insemination (AI) program in Fatick (Senegal).

Materials and Methods: Of 193 candidate goats in AI program, 167 were selected (day 50) in six villages. Estrus was synchronized by progesterone impregnated sponges installed for 11 days. Two days before the time of sponge removal (day 4), each goat was treated with 500 IU of equine chorionic gonadotropin and 50 μg of dcloprostenol. All goats were inseminated (day 0) with alpine goat semen from France at 45±3 h after sponge removal (day 2). Real-time B-mode ultrasonography was performed at day 50, day 13, day 0, day 40 and day 60 post-AI.

Results: Selection rate, estrus response rate, AI rate, PR at days 40 and days 60 were 86.53%; 71.85%; 83.34%; 51% and 68% (p<0.05) respectively. Value of sensitivity, specificity, positive and negative predictive value, accuracy, total conformity, conformity of correct positive, conformity of correct negative and discordance of pregnancy diagnosis by trans-abdominal ultrasonography (TU) were 98.03%; 63.26%; 73.52%; 3.12%; 81%; 81%; 50%; 31% and 19%, respectively.

Conclusion: These results indicate that the TU can be performed in goats under traditional condition and emphasized the importance of re-examination of goats with negative or doubtful TU diagnoses performed at day 40 post-AI.

Keywords: accuracy, artificial insemination, estrus, pregnancy, Sahelian goats, ultrasonography.



1. Purohit, G. (2010) Methods of pregnancy diagnosis in domestic animals: The current status. Web. Med. Cent. Repro., 1(12): WMC001305.
 
2. Torloni, M.R., Vedmedovska, N., Merialdi, M., Betrán, A.P., Allen, T., González, R. and Platt, L.D. (2009) Safety of ultrasonography in pregnancy: WHO systematic review of the literature and meta-analysis. Ultrasound Obstet. Gynecol., 33(5): 599-608.
http://dx.doi.org/10.1002/uog.6328
PMid:19291813
 
3. Moraes, E.P.B.X., Santos, M.H.B., Arruda, I.J., Bezerra, F.Q.G., Filho, A.C.R., Neves, J.P., Lima, P.F. and Oliveira, M.A.L. (2007) Hydrometra and mucometra in goats diagnosed by ultrasound and treated with PGF2α. Med. Vet., 1: 33-39.
 
4. Suguna, K., Mehrotra, S. and Agarwal, S.K. (2008) Early pregnancy diagnosis and embryonic and fetal development using real time B mode ultrasound in goat. Small Rum. Res., 80: 80-86.
http://dx.doi.org/10.1016/j.smallrumres.2008.10.002
 
5. Padilla-Rivas, G.R., Sohnrey, B. and Holtz, W. (2004) Early pregnancy detection by real-time ultrasonography in Boer goats. Small Rum. Res., 58(1): 87-92.
http://dx.doi.org/10.1016/j.smallrumres.2004.09.004
 
6. Hesselink, J.W. and Taverne, M.A.M. (1994) Ultrasonography of the uterus of the goat. Vet. Q., 16: 41-45.
http://dx.doi.org/10.1080/01652176.1994.9694415
PMid:8009818
 
7. Mialot, J.P., Levy, I. and Emery, P. (1991) Echographie et gestion des troupeaux caprins. Rec. Méd. Vét., 167: 399-406.
 
8. Djakba, A. (2007) Evaluation of reproduction parameters of the inseminated Sahelian goats and the growth of the kids in the area of Fatick in Senegal. Vet. Thesis Dakar. p. 87.
 
9. González, F., Cabrera, F., Batista, M., Rodriguez, N., Sulon, J. and Beckers, J.S. (2004) A comparison of diagnosis of pregnancy in the goat via transrectal ultrasound scanning, progesterone, and pregnancy-associated glycoprotein assays. Theriogenology, 62(6): 1108-1115.
http://dx.doi.org/10.1016/j.theriogenology.2003.12.009
PMid:15289050
 
10. Hanzen, C.H., Pieterse, M., Szenci, O. and Drost, M. (2000) Relative accuracy of the identification of ovarian structures in the cow by ultrasonography and palpation per rectum. Vet. J., 159: 161-170.
http://dx.doi.org/10.1053/tvjl.1999.0398
PMid:10712804
 
11. Snedecor, G.W. and Cochran, W.G. (1989) In: Statistical Methods. 8th ed. Iowa State University Press, Ames, Iowa, USA. p125-128.
 
12. Mpatswenumugabo, J.P. (2009) Monitoring and evaluation of the caprine artificial insemination quality in the rural area of Fatick in Senegal. Vet. Thesis Dakar. p. 122.
 
13. Kor, N.M., Khanghah, M.K. and Ali, A. (2012) Efficiency of short time protocols based on combined FGA, PGF2α, GnRH and eCG treatments on oestrus synchronization and reproductive performance of kermani ewes during the breeding season. Int. J. Biol. Med. Res., 3(3): 1966-1970.
 
14. Omontese, B.O., Rekwot, P.I., Ate, I.U., Rwuaan, J.S. and Makun, H.J. (2013) Comparative estrus induction in indigenous Sahel goats using two progestagens (CIDR and FGA) and eCG. Livest. Res. Rural Dev., 25. Available from: http://www.lrrd.org/lrrd25/4/omon25066.htm (25-04-2014).
 
15. Kouamo, J., Safari, T., Miguiri, K., Sandeu, M.M. and Sawadogo, G.J. (2014) Study of nutritional parameters in Sahelian goats inseminated at induced estrus (FGA sponge and eCG). Livest. Res. Rural Dev., 26. Available from: http://www.lrrd.org/lrrd26/3/koua26038.html (25-04-2014).
 
16. Romano, J.E. (2002) Does in proestrus-estrus hasten estrus onset in does estrous synchronized during the breeding season. Appl. Anim. Behav. Sci., 77: 329-344.
http://dx.doi.org/10.1016/S0168-1591(02)00064-3
 
17. Karen, A., Szabados, K., Reiczigel, J., Beckers, J.F. and Szenci, O. (2004) Accuracy of transrectal ultrasonography for determination of pregnancy in sheep: Effect of fasting and handling of the animals. Theriogenology, 61: 1291-1298.
http://dx.doi.org/10.1016/j.theriogenology.2003.07.018
PMid:15036963
 
18. Kouamo, J. and Sawadogo, G.J. (2012) Synchronization rate and factors affecting pregnancy rate after synchronization of estrus cycle and insemination of Gobra zebu (Bos indicus) in traditional area in Senegal. Livest. Res. Rural Dev., 24. Available from: http://www.lrrd.org/lrrd24/11/koua24200.htm (25-04-2014).
 
19. Omontese, B.O., Rekwot, P.I., Makun, H.J., Obidi, J.A., Rwuaan, J.S. and Chiezey, N.P. (2010) Evaluation of EAZI-BREEDTM CIDR® and FGA-30® intravaginal sponges as synchronizing agents in prepartum red Sokoto Does. J. Vet. Res., 3(3): 64-69.
 
20. Omontese, B.O., Rekwot, P.I., Makun, H.J., Ate, I.U. and Rwuaan, J.S. (2012) Induction of estrus in Sahel goats using fluorogestone acetate (FGA) sponges and equine chorionic gonadotrophin (eCG). Sokoto J. Vet. Sci., 10(2): 21-25.
 
21. Mbaidingatoloum, F.M. (2003) A protocol of artificial insemination of the Sahelian goats in field conditions: preliminary results. Post graduate diploma in animal productions, Dakar (EISMV), p30.
 
22. Rwakazina, O. (2005) Evaluation of the productivity in field and station of the Boer goat in Rwanda. Vet. Thesis Dakar. p. 92.
 
23. Bretzlaff, K.N. and Romano, J.E. (2001) Advanced reproductive techniques in goats. Vet. Clin. North Am. Food Anim. Pract., 17: 421-434.
PMid:11515409
 
24. Davey, C.G. (1986) An evaluation of pregnancy testing in sheep using a real-time ultrasound scanner. Aust. Vet. J., 63: 347-348.
http://dx.doi.org/10.1111/j.1751-0813.1986.tb02889.x
PMid:3541886
 
25. Taverne, M.A.M., Szenci, O., Szetag, J. and Piors, A. (1985) Pregnancy diagnosis in cows with linear-array real time ultrasound scanning. A preliminary note. Vet. Q., 7: 264-270.
http://dx.doi.org/10.1080/01652176.1985.9693998
PMid:3907117
 
26. Medan, M., Watanabe, G., Absy, G., Sasaki, K., Sharawy, S. and Taya, K. (2004) Early pregnancy diagnosis by means of ultrasonography as a method of improving reproductive efficiency in goats. J. Reprod. Dev., 50(4): 391-7.
http://dx.doi.org/10.1262/jrd.50.391
PMid:15329470
 
27. Jardon, C., De Montigny, G., Andre, D., Corteel, J.M., Baril, G., Cognie, Y., Botero-Herrera, O. and Humblot, P. (1984) Methods of pregnancy diagnosis in sheep and goats. In IXe Research days in sheep and goats. INRA-ITOVIC, Paris. p452-473.
 
28. Fowler, D.G. and Wilkins, J.F. (1984) Diagnosis of pregnancy and number of the fetuses in sheep by real-time ultrasonic imaging. 1. Effect of number of fetuses, stage of gestation, operator and breed of ewe on accuracy of diagnosis. Livest. Prod. Sci., 11: 437-450.
http://dx.doi.org/10.1016/0301-6226(84)90055-1
 
29. Roberts, S.J. editor. (1971) Infertility in ewes and does. Veterinary Obstetrics and Genital Diseases. Edwards Brothers Inc., Ann Arbor, Ml. p579.
 
30. Sharky, S., Callan, R.J., Mortimer, R. and Klimberling, C. (2001) Reproductive techniques in sheep. Vet. Clin. North Am. Food Anim. Pract., 17: 235-255.