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Research
(Published
online: 30-09-2014)
23. Reflection of serum
immunoglobulin isotypes in the egg yolk of laying hens immunized
with enterotoxigenic
Escherichia coli -
Nagendra Nath Barman, Anjan Jyoti Nath and
Niranjan Kalita
Veterinary World, 7(9): 749-753
doi:
10.14202/vetworld.2014.749-753
Nagendra Nath Barman:
Department of
Microbiology, College of Veterinary Science, Khanapara, Guwahati,
Assam, India;
nnbarman@gmail.com
Anjan
Jyoti Nath:
Pasteur Institute of India, Coonoor, Nilgiris, Tamil Nadu, India;
drnath76@gmail.com
Niranjan Kalita: Department of Poultry Science, College of
Veterinary Science, Assam Agricultural University, Khanapara,
Guwahati, Assam, India;
niranjankalita@gmail.com
Received:
27-05-2014, Revised: 10-08-2014, Accepted: 19-08-2014, Published
online: 30-09-2014
Corresponding author:
Nagendra Nath Barman, e-mail: nnbarman@gmail.com
Abstract
Aim:
The aim was to study the seroconversion and development of egg
yolk immunoglobulins in adult laying White Leghorn hens immunized
against an isolate of enterotoxigenic Escherichia coli (ETEC)
bearing K91 and K88ac antigens, obtained from diarrheic piglet.
Materials and Methods: Adult laying White Leghorn hens were
immunized with inactivated enterotoxic E. coli strain
isolated originally from a case of piglet diarrhea following
recommended schedule. The development of whole antibodies and
isotype-specific antibodies in serum and egg yolk were measured
using indirect enzyme-linked immunosorbent assay (ELISA). Piglets
suffering from diarrhea with fecal samples positive for ETEC were
fed with egg yolk and compared with diarrheic control group.
Results: The serum and egg yolk ELISA antibody titer against
E. coli strain used in the present study was as high as
2666.66±307.92 and 933.33±203.67 respectively on 50
day-post-vaccination (DPV). The immunoglobulin Y (IgY) was the
predominant isotype in serum and egg yolk, which reached the peak
titer of 2200±519.61 in serum on 40 DPV and 800±244.94 in egg yolk
on 50 DPV. IgM titer in serum and egg yolk was found to be meager,
and no IgA could be detected. Diarrheic piglets fed with the egg
yolk suspension from immunized hens showed a promising result in
controlling diarrhea.
Conclusion: Egg yolk antibodies are considered a suitable
immunotherapeutic alternative to conventional antibiotic therapy.
High titer of egg yolk antibodies raised in the immunized hen
against an isolate of ETEC holds the potential to be used for
passive protection of diarrheic piglets during their most
susceptible period of infection.
Keywords: egg yolk antibodies, enterotoxigenic Escherichia
coli, immunization.
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