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Research
(Published
online: 06-09-2014)
3.
Relationships among frozen-thawed semen
fertility, physical parameters, certain routine sperm
characteristics and testosterone in breeding Murrah buffalo (Bubalus
bubalis) bulls -
A. K. Singh, P. S.
Brar and R. S. Cheema
Veterinary World, 7(9): 644-651
doi:
10.14202/vetworld.2014.644-651
A. K. Singh: Department of
Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary
and Animal Sciences University, Ludhiana - 141 004, Punjab, India;
assengar2001@yahoo.co.in
P. S. Brar: Department of Veterinary
Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana - 141 004, Punjab, India;
PSB: parkashbrar@gmail.com
R. S. Cheema; Department of Veterinary
Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana - 141 004, Punjab, India;
ranjna.cheema@gmail.com
Received:
23-04-2014, Revised: 07-07-2014, Accepted: 12-07-2014, Published
online: 06-09-2014
Corresponding author: A. K. Singh,
e-mail: assengar2001@yahoo.co.in
Abstract
Aim:
The present study was carried out to examine the relationships
among frozen-thawed semen fertility, physical parameters, seminal
quality, and testosterone concentration in Murrah buffalo bulls.
Materials and Methods: A total of 30 breeding Murrah buffalo
bulls (either progeny tested or under progeny testing program)
were randomly selected from two government bull farms in Punjab.
None of the bulls selected for this study had any preceding
physical abnormality. A field fertility trial was conducted to
determine the first service conception rate (FSCR). The number of
females inseminated per bull semen was 10. All the bulls were
inspected for structural soundness, measurement of scrotal
circumference, testicular biometry, and internal pelvic area
(IPA). Frozen-thawed semen was evaluated for total motility,
progressive motility, viability, concentration, abnormality, and
hypo-osmotic swelling test (HOST). Testosterone was estimated in
blood plasma, seminal plasma as well as frozen-thawed semen
extracts for establishing relationship.
Results: The FSCR was 48% in the bulls having a scrotal
circumference of ≥44
cm, although, there was no significant correlation between FSCR
and scrotal circumference. Similarly, no consistent relationship
existed between sperm concentration and scrotal circumference. A
positive correlation was observed between IPA and FSCR (r=0.294).
Of the six post-thaw seminal components (total motility,
progressive motility, viability, HOST (%), total abnormality and
concentration) only total motility had a high significant (p<0.01)
correlation with FSCR (r=0.694). Varied correlations existed
between other seminal parameters and fertility. Using a simple
regression analysis, the post-thaw motility, IPA, prepuce length
and testosterone (independent variables) combined to explain
approximately 62% of the variation in the FSCR (dependent
variable).
Conclusion: The present study indicated that despite low to
high correlations between seminal characteristics, physical
parameters, fertility, and testosterone; the observations support
the importance of these components and their function in
maintaining semen quality and subsequent fertility.
Keywords: buffalo
spermatozoa, fertility, pelvic area, scrotal circumference,
testosterone.
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